Liver tissue (lobe 1) was obtained from 10-day-old mice and subjected to exhaustive solvent extraction for lipids. Global lipidomics analysis was performed on a Xevo G2-S quadrupole time-of-flight (Q-TOF) mass spectrometer (MS) interfaced with an Acquity ultra-high performance liquid chromatography (UPLC) system (Waters, Milford, MA) operated in electrospray ionization mode. An Acquity CSH C18 UPLC column was used to chromatographically separate components over 20 minute gradient elution. Compounds were ionized with electrospray and positive and negative ions acquired over the mass range 50-1200 Daltons with high resolution. Deconvolution, peak alignment, and preliminary normalization were conducted on raw metabolomics data with Progenesis QI (Waters). Each compound ion feature was annotated by elution time with m/z. Raw data were normalized by total compound ion intensity and with a global scalar derived from logarithm ratio of each sample to the reference. Accurate molecular mass (m/z) was used to search against HMDB and lipid MAPS database for putative identification.
Xevo g2 s quadrupole time of flight q tof mass spectrometer
Manufactured by Waters Corporation
The Xevo G2-S quadrupole time-of-flight (Q-TOF) mass spectrometer (MS) is a high-resolution mass spectrometry instrument designed for advanced analytical applications. It combines a quadrupole mass analyzer with a time-of-flight (TOF) mass analyzer, providing accurate mass measurement and high-resolution capabilities. The Xevo G2-S Q-TOF MS is capable of performing both full-scan and targeted analyses to enable comprehensive characterization of complex samples.
Automatically generated - may contain errors
2 protocols using xevo g2 s quadrupole time of flight q tof mass spectrometer
1
Global Lipidomics of Murine Liver
2
Global Lipidomics of Murine Liver
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