Immunohistochemical staining was performed either on 3-μm sections from formalin-fixed, paraffin-embedded tissue samples or on a tissue microarray with FTE and OC samples from patients operated at the European Institute of Oncology in Milan between 2014 and 2016. The staining was performed as described previously (Francavilla et al., 2017 (link)), after antigen retrieval with Tris-EDTA (pH 9.0), at 95°C for 50 min. The rabbit monoclonal anti-CD73 (clone D7F9A, Cell Signaling Technology, catalog no. 13160) was used as primary antibody. CD73 signal was analyzed and scored by a trained pathologist (G.B.).
Anti cd73 clone d7f9a
Manufactured by Cell Signaling Technology
Anti-CD73 (clone D7F9A) is a laboratory reagent used for the detection and analysis of CD73 (also known as ecto-5'-nucleotidase) expression. CD73 is a cell surface enzyme involved in the conversion of extracellular adenosine monophosphate (AMP) to adenosine. This reagent can be used in various applications such as flow cytometry, immunohistochemistry, and Western blotting to identify and study cells expressing CD73.
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Lab products found in correlation
2 protocols using anti cd73 clone d7f9a
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Immunohistochemical Staining of CD73
2
Immunohistochemical Analysis of Colon Cancer Markers
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The experiments on paraffin-embedded colonic tissues and TMAs were approved by umbrella spore IRB no. 070166. Tumour xenografts were fixed in neutralized formalin and embedded in paraffin. Slices were deparaffinized with serial histoclear and ethanol. Antigen retrieval was performed in citrate buffer (pH 6.0) with high pressure at 110 °C for 15 min and then quenched in 0.03% H202 with sodium azide for 5 min. The slides were incubated with primary antibodies at room temperature for 60 min and then incubated in Dako Envision + system horseradish peroxidase-labelled polymer at room temperature for 30 min. Signal was detected by incubating in a DAB+ substrate chromogen system at room temperature for 5 min. The primary antibodies used were: anti-DPEP1 (1:1,000; Sigma-Aldrich, HPA012783), anti-CD73 (clone D7F9A, 1:300; Cell Signaling Technology, 13160), anti-TGFBI (clone EPR12078(B), 1:300; Abcam, ab170874), anti-FASN (clone G-11, 1:500; Santa Cruz Biotechnology, sc-48357) and anti-AGO2 (clone EPR10411, 1:500; Abcam, ab57113).
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