Prostar 360 detector

Manufactured by Agilent Technologies

Sourced in United States

The ProStar 360 detector is a versatile and reliable instrument designed for use in various analytical applications. It functions as a high-performance detector, capable of accurately measuring and quantifying target analytes in complex sample matrices. The core function of the ProStar 360 is to provide precise and reproducible detection of compounds, enabling researchers and analysts to obtain reliable data for their specific research or testing needs.

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Lab products found in correlation

Spd 6a uv detector, by Shimadzu (3 mentions) Rp 18 column, by Merck Group (2 mentions) Kynurenine and tryptophan standards, by Merck Group (1 mentions) 3 nitro l tyrosine, by Merck Group (1 mentions)

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ProStar 360 (3 citations)

3 protocols using prostar 360 detector

Quantifying Tryptophan Metabolism Markers

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Aliquots from the frozen plasma samples were used for these investigations.
Time points zero, 30 min after the last inhalation and 1 week after the last
inhalation were taken. Neopterin concentrations were determined by
enzyme-linked immunosorbent assay with a detection limit of 2 nmol/l
(Brahms, Berlin, Germany).
Serum tryptophan and kynurenine concentrations were assessed by
reversed-phase HPLC on a ProStar Varian system (USA) according to the
protocol described earlier.^{27 (link)} In brief, serum specimens were deproteinized with trichloroacetic
acid and separated on reversed-phase C18 material using 15 mmol/l acetic
acid-sodium acetate buffer (pH 4.0) and a flow rate of 0.9 ml/min.
Tryptophan was monitored by means of its native fluorescence at 286 nm
excitation and 366 nm emission wavelengths (ProStar 360 detector, Varian,
USA). Kynurenine was detected by ultraviolet-absorption at 360 nm wavelength
(Shimadzu SPD-6A UV detector, Austria) in the same chromatographic run.
Finally, kynurenine to tryptophan (Kyn/Trp) ratio was calculated as an
indirect estimate of indoleamine 2,3- dioxygenase activity by dividing
kynurenine concentrations (μmol/l) by tryptophan concentrations
(mmol/l).

Arnitz R., Stein M., Bauer P., Lanthaler B., Jamnig H., Scholl-Bürgi S., Stempfl-Al-Jazrawi K., Ulmer H., Baumgartner B., Embacher S., Geisler S., Gostner J.M., Müllinger B., Kälz B, & Nagl M. (2018). Tolerability of inhaled N-chlorotaurine in humans: a double-blind randomized phase I clinical study. Therapeutic Advances in Respiratory Disease, 12, 1753466618778955.

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Serum Tryptophan and Kynurenine Analysis

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Serum Trp and Kyn concentrations were measured by reversed-phase high-pressure liquid chromatography on a ProStar Varian system, as described earlier [32 (link), 33 (link)]. In brief, chromatographic separation was performed using a RP-18 column (Merck, Germany) and 15 mmol/l acid-sodium acetate buffer (pH 4.0) as eluent (flow rate: 0.9 ml/min). Serum specimens were deproteinized with trichloroacetic acid (2 mol/l) before analysis. 3-Nitro-L-tyrosine was used as an internal standard. All chemicals were purchased from Sigma-Aldrich (Austria). Trp was monitored by its native fluorescence at 286 nm excitation and 366 nm emission wavelengths (ProStar 360 detector, Varian, USA); Kyn and 3-nitro-L-tyrosine were detected by UV absorbance at the 360 nm wavelength (Shimadzu SPD-6A UV detector, Austria) in the same chromatographic run. Finally, the Kyn to Trp ratio (Kyn/Trp; kynurenine (μmol/l) divided by Trp (mmol/L)) was calculated [6 (link)].

Sucher R., Hautz T., Mohr E., Mackowitz M., Mellitzer V., Steger C., Cardini B., Resch T., Margreiter C., Schneeberger S., Brandacher G., Fuchs D., Oberhuber R, & Gostner J.M. (2019). Sodium Sulfite Exacerbates Allograft Vasculopathy and Affects Tryptophan Breakdown in Murine Heterotopic Aortic Transplantation. Oxidative Medicine and Cellular Longevity, 2019, 8461048.

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HPLC Analysis of Tryptophan and Kynurenine

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Briefly, the HPLC analysis of tryptophan and kynurenine was performed on a ProStar Varian system (USA) using rp-18 columns (Merck, Germany) and acetate buffer as eluent (flow-rate: 0.9 mL/min) according to the protocol described earlier [26 (link), 27 (link)]. 3-Nitro-L-tyrosine (Sigma Aldrich, Austria) was used as an internal standard. Kynurenine and tryptophan standards were purchased from Sigma-Aldrich (Austria). Kynurenine and 3-Nitro-L-tyrosine were detected by UV-absorbance at 360 nm wavelength (Shimadzu SPD-6A UV detector, Austria), tryptophan was detected by its fluorescence with an excitation wavelength of 286 nm and an emission wavelength of 366 nm (ProStar 360 detector, Varian, USA). The Kyn/Trp was calculated, which is an estimate of IDO-1 activity [12 (link)] and expressed in μmol Kyn / mmol Trp. The sensitivity of the measurements was 0.5 μmol/L kynurenine and 0.1 μmol/L tryptophan.

Becker K., Schwaiger S., Waltenberger B., Fuchs D., Pezzei C.K., Schennach H., Stuppner H, & Gostner J.M. (2018). Immunomodulatory Effects of Diterpene Quinone Derivatives from the Roots of Horminum pyrenaicum in Human PBMC. Oxidative Medicine and Cellular Longevity, 2018, 2980295.

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