O 2 3 4 5 6 pentafluorobenzyl hydroxylamine hydrochloride

Precisa LS 220A SCS (Dietikon, Switzerland) analytical balance was used for weighing. GC-FID analysis was performed with Shimadzu GC-2010 Plus (Kyoto, Japon) equipped with Shimadzu AOC-5000 Plus (Kyoto, Japon) autosampler and Restek Rtx-Wax capillary column (30 m × 0.32 mm ID, 0.25 μm film thickness) (Pennsylvania, United States). For absorption measurements of the validation method, Shimadzu UV-1800 (Kyoto, Japon) spectrophotometer was used.
Formaldehyde standard, o-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloride as derivatization reagent, 1-bromo-4-fluorobenzene (BFB) as internal standard were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Sodium chloride, potassium chloride, calcium chloride dihydrate, acetylacetone, acetic acid (glacial) were provided from Merck KGaA (Darmstadt, Germany).

All chemicals were purchased and used without any purification: 1-penten-3-ol (Sigma Aldrich, 99.0 %), (Z)-2-hexen-1-ol (Sigma Aldrich, 95.0 %), (E)-2-hexen-1-al (Sigma Aldrich, 98.0 %), catalase from bovine liver (Sigma Aldrich, 10,000–40,000 units/mg protein), o-(2,3,4,5,6- pentafluorobenzyl) hydroxylamine hydrochloride (Sigma Aldrich, for GC derivatization, ≥ 99 % (AT)), hydrogen peroxide (H₂O₂, CHEMSOLUTE, 30.0 % wt. in H₂O), acetonitrile (Optima, LC-MS grade), fuming hydrochloric acid (EMSURE, 37.0 %), 2,2,6,6-cyclohexanone-d₄ (ISOTEC, 99 atom % D), 2,2,4-trimethyl pentane or isooctane (Merck, p.a. ≥ 99.5 % GC), formic acid LC-MS grade (Merck LiChropur, 98–100 % and Fisher Optima). Dichloromethane (Honeywell, ≥ 99.0 %), 1-penten-3-one (Sigma Aldrich, analytical standard with 0.1 % BHT as stabilizer), propionaldehyde (Merck, ≥ 98 %), butyraldehyde (Merck, ≥ 99 %), trans-2-hexenoic acid (Sigma Aldrich, 99 %). Aqueous solutions were freshly prepared using Milli-Q water (resistivity 18.2 MΏ cm, TOC < 3 ppb, Elix Millipore, Milli-Q gradient A10).

Precisa LS 220A SCS (Dietikon, Switzerland) analytical balance was used for weighing. GC-FID analysis was performed with Shimadzu GC-2010 Plus (Kyoto, Japon) equipped with Shimadzu AOC-5000 Plus (Kyoto, Japon) autosampler and Restek Rtx-Wax capillary column (30 m × 0.32 mm ID, 0.25 μm film thickness) (Pennsylvania, United States). For absorption measurements of the validation method, Shimadzu UV-1800 (Kyoto, Japon) spectrophotometer was used.
Formaldehyde standard, o-(2,3,4,5,6-Pentafluorobenzyl)hydroxylamine hydrochloride as derivatization reagent, 1-bromo-4-fluorobenzene (BFB) as internal standard were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Sodium chloride, potassium chloride, calcium chloride dihydrate, acetylacetone, acetic acid (glacial) were provided from Merck KGaA (Darmstadt, Germany).