Anti β tubulin tub 2.1

Anti-FLAG rabbit polyclonal antibodies and the anti-FLAG (M2) and anti-β-tubulin (TUB 2.1) mouse monoclonal antibodies were purchased from Sigma-Aldrich; the anti-FLAG mouse monoclonal antibody (1E6) from FUJIFILM Wako Pure Chemical Corporation; the anti-HA mouse monoclonal antibody (16B12) from Covance; the anti-ZO-1 rat monoclonal antibody (R40.76), anti-β-catenin (H-102), anti-HA (Y-11), and anti-Myc (A14) rabbit polyclonal antibodies from Santa Cruz Biotechnology; the anti-Myc (9E10) mouse monoclonal and anti-HA (3F10) rat monoclonal antibodies from Roche Applied Science; anti-MBP rabbit polyclonal antibodies from New England Biolabs; anti-Par3 rabbit polyclonal antibodies from EMD Millipore; the anti-occludin mouse monoclonal antibody (OC-3F10), anti-ZO-1, anti-claudin-1, and anti-claudin-2 rabbit polyclonal antibodies, and Alexa Fluor 647-conjugated anti-ZO-1 mouse monoclonal antibody (ZO-1A12) from Thermo Fisher Scientific; the anti-E-cadherin rabbit monoclonal antibody (24E10) and anti-phospho-myosin light chain 2 (MLC2) (Thr18/Ser19) rabbit polyclonal antibodies from Cell Signaling Technology; anti-TMEM25 rabbit polyclonal antibodies (A13805) from Boster; and the anti-GFP rat monoclonal (GF090R) or mouse monoclonal (GF200) antibodies from Nacalai Tesque.

Immunoblotting was performed as previously described [32 (link)]. The following antibodies were used in this study: anti-MCPyV-LT (CM2B4; Santa Cruz Biotechnology, Dallas, TX, USA), anti-MCPyV-TA (2T2 hybridoma supernatant; [34 (link)]), anti-AURKA (1G4; Cell Signaling, Danvers, MA, USA), anti-β-tubulin (TUB 2.1; Sigma-Aldrich, Taufkirchen, Germany) and anti-vinculin (V9131; Sigma Aldrich).