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2 protocols using d fructose 6 p

1

Analytical Standards for Biochemical Analysis

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d-mannitol, d-sorbitol, d-arabitol, xylitol, ribitol, erythritol, glycerol, d-fructose-6-P, and d-fructose were obtained from Sigma-Aldrich (St. Louis, United States). Coomassie brilliant blue R-250, cofactors NADP, NAD, NADH, NADPH, and antibiotics were purchased from Sangon Biotech (Shanghai, China). New England Biolabs or Takara supplied all restriction enzymes. High-fidelity KOD-Fx, 2 × ready-to-use qTaq mixture for PCR were purchased from Toyobo (Japan). RNA extraction kit, reverse transcription kit, and qPCR kit were obtained from Vazyme Biotech Co., Ltd (Nanjing, China). All other chemicals were of analytical grade.
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2

Purification and Analysis of Enzyme Intermediates

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Lysogeny broth (LB) medium, isopropyl β-d-thiogalactopyranoside (IPTG), and NADPH were purchased from
Research Products International. The protease inhibitor cocktail,
lysozyme, DNase I, acetaldehyde dehydrogenase, pyrophosphatase, d-fructose-6-P, d-fructose-1-P, d-glucose-6-P, d-glucose-1-P, d-glucitol-6-P, CTP, UTP, ATP, GTP,
NADPH, NADH, acetaldehyde, kanamycin, imidazole, and HEPES were obtained
from Sigma-Aldrich. Vivaspin 20 spin filters and HisTrap and HiTrap
Q columns were obtained from Cytiva. The 10 kDa Nanosep spin filters
were purchased from Pall Corp. (Port Washington, NY). Deuterium oxide
was acquired from Cambridge Isotope Laboratories Inc.
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