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Aflatoxins b1

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Aflatoxins B1 is a laboratory product used for analytical and research purposes. It is a naturally occurring mycotoxin produced by certain fungi that can contaminate various food and feed commodities. The core function of Aflatoxins B1 is to serve as a reference standard for the detection and quantification of aflatoxin levels in samples.

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Lab products found in correlation

Horseradish peroxidase hrp, by Merck Group (1 mentions) Magnesium sulphate, by Merck Group (1 mentions) Dimethylformamide dmf, by Merck Group (1 mentions) Primary secondary amine bulk sorbent, by Agilent Technologies (1 mentions) Sigma 4k10 centrifuge, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Tween 20, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Sulphuric acid, by Merck Group (1 mentions) Keyhole limpet hemocyanin (klh), by Merck Group (1 mentions) Sodium borohydride, by Merck Group (1 mentions) N heptane, by Merck Group (1 mentions)

Spelling variants of this product

Aflatoxin B1 (67 citations) Aflatoxin B1 (AFB1) (14 citations) Aflatoxins (7 citations) Aflatoxin B1 standard (3 citations) Aflatoxin B1 (AFB1) standard solution (2 citations) Aflatoxin B1-Bovine Serum Albumin (AFB1-BSA), (2 citations) Anti-Aflatoxin B1 (2 citations)

4 protocols using aflatoxins b1

1

Radiolabeled Aflatoxin B1 Protocol

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The standard labeled aflatoxin (AFB13H) was purchased from Moravek Biochemical (Brea, California, USA). Other standards of aflatoxins B1, B2, G1 and G2 were acquired from Sigma Aldrich (Sao Paulo, Brazil). Insta Gel solution was purchased from Pelkin Elmer Radiochemicals (Boston, MA, USA). Individual doses were prepared from a standard solution of AFB13H (9.25 × 106 Bq) labeled aflatoxin B1, containing 4.7 µg of aflatoxin B1 (16.6 Ci/mmol) diluted in 0.5 mL of 8% sulphoxide dimethyl (Moravek Biochemical, Brea, California, USA). Hereafter, AFB13H refers to Aflatoxin B1 radiolabeled with 3H. Bq (Becquerel) is the international system of units for radioactivity. One Bq is the activity of a quantity of radioactive material in which one nucleus disintegrates per second.
The other reagents and solutions used in this study were purchased from Merck S.A. (Sao Paulo, Brazil).
Sica P., Domingues M.A., Mota L.A., Pinto A.U., Baptista A.A., Horii J., Abdalla A.L, & Baptista A.S. (2024). How does active yeast supplementation reduce the deleterious effects of aflatoxins in Wistar rats? A radiolabeled assay and histopathological study. World Journal of Microbiology & Biotechnology, 40(6), 164.
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2

Aflatoxin B1 Spiked Peanut Analysis

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Salted roasted peanuts (Bistro-peanuts with USA origin, Aligro, Switzerland) were purchased from a local store, as well as peanut oil (Florin, Aligro, Switzerland) . Ginger, chili pepper, garlic, pepper, mint, and laurel powders, as well as unhusked sesame seeds were bought from another local store (Naturaplan, COOP, Switzerland), moringa leaf powder (Moringa oleifera) was purchased online (Morbalance, Switzerland). Aflatoxins B1 (powder form) was purchased from Sigma Aldrich (Buchs, Switzerland) and was dissolved into methanol at a concentration of 50 mg/L. Aliquots of 2 ml were then prepared and stored in the dark at -20 C until use to avoid any toxin degradation.
Gard C., Kolich Chong M.A., Sawadogo Y.A, & Moreau L. (2021). Plant addition impact on aflatoxin B1 levels, physico‐chemical, nutritional, and sensory properties of fried peanut biscuits. Journal of food safety., 41(3).
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3

Aflatoxin B1 Spiked Peanut Analysis

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Salted roasted peanuts (Bistro-peanuts with USA origin, Aligro, Switzerland) were purchased from a local store, as well as peanut oil (Florin, Aligro, Switzerland) . Ginger, chili pepper, garlic, pepper, mint, and laurel powders, as well as unhusked sesame seeds were bought from another local store (Naturaplan, COOP, Switzerland), moringa leaf powder (Moringa oleifera) was purchased online (Morbalance, Switzerland). Aflatoxins B1 (powder form) was purchased from Sigma Aldrich (Buchs, Switzerland) and was dissolved into methanol at a concentration of 50 mg/L. Aliquots of 2 ml were then prepared and stored in the dark at -20 C until use to avoid any toxin degradation.
Gard C., Chong M.A.K., Sawadogo Y.A., & Moreau L. (2021). Plant addition impact on aflatoxin B1 levels, physico‐chemical, nutritional, and sensory properties of fried peanut biscuits. Journal of Food Safety, 41(3).
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4

Quantifying Aflatoxins in Food Samples

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STG, aflatoxins (B1, B2, G1, G2 and M1), acetonitrile (ACN), methanol (MeOH), acetone, chloroform, n-heptane, sulphuric acid, disodium hydrogen phosphate, sodium dihydrogen phosphate, potassium dihydrogen phosphate, sodium carbonate, sodium bicarbonate, sodium chloride, magnesium sulphate, Tween 20, bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), horseradish peroxidase (HRP), sodium borohydride and dimethylformamide (DMF) were purchased from Sigma-Aldrich (Dorset, UK & Zwijndrecht, the Netherlands). Primary-secondary amine (PSA) bulk sorbent was purchased from Agilent (Amstelveen, the Netherlands). Substrate 3,3',5,5'-tetramethylbenzidine (TMB) for HRP enzyme was obtained from Neogen (Lansing, USA).
Samples were blended using IKA A11 Basic laboratory mill (IKA, Staufen, Germany) and centrifuged in Sigma 4K10 centrifuge (Sigma, Osterode am Harz, Germany). Microtiter plates were read on BioTek EL808 type ELISA plate reader (BioTek, Bad Friedrichshall, Germany).
Oplatowska-Stachowiak M., Reiring C., Sajic N., Haasnoot W., Brabet C., Campbell K., Elliott C.T, & Salden M. (2018). Development and in-house validation of a rapid and simple to use ELISA for the detection and measurement of the mycotoxin sterigmatocystin. Analytical and bioanalytical chemistry, 410(12).
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