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Hitrap nhs activated affinity column

Manufactured by GE Healthcare
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The HiTrap NHS-activated affinity column is a lab equipment product designed for protein purification. It is a prepacked column that allows for the covalent immobilization of ligands containing primary amino groups. The column is used in the process of affinity chromatography to separate and purify specific proteins from complex mixtures.

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Lab products found in correlation

Hitrap, by Cytiva (2 mentions) Bac to bac, by Thermo Fisher Scientific (1 mentions) Pi secsumostar vector, by LifeSensors (1 mentions)

Close spelling variants of this product

HiTrap columns (11 citations) HiTrap NHS column (6 citations) NHS column (5 citations) HiTrap NHS-Activated HP affinity column (5 citations) NHS-activated HiTrap (4 citations)

2 protocols using hitrap nhs activated affinity column

1

FAMK-1 Protein Purification and Antibody Generation

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FAMK-1 (residues 60–512) was expressed using a modified pI-secSUMOstar vector (LifeSensors) and purified from Hi5 insect cells (Bac-to-Bac, B85502; Invitrogen; Xiao et al., 2013 (link)). The purified protein was sent to Cocalico Biologicals for immunizing rabbits. FAMK-1–specific antibodies were affinity purified from serum with a HiTrap NHS-activated affinity column (17-0716-01; GE Healthcare) on which FAMK-1 was immobilized.
Gerson-Gurwitz A., Worby C.A., Lee K.Y., Khaliullin R., Bouffard J., Cheerambathur D., Oegema K., Cram E.J., Dixon J.E, & Desai A. (2019). Ancestral roles of the Fam20C family of secreted protein kinases revealed in C. elegans. The Journal of Cell Biology, 218(11), 3795-3811.
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2

Properdin Depletion by mAb 1340

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MAb 1340 was coupled to a HiTrap NHS-activated affinity column (GE Healthcare Life Science, Piscatawa, USA) according to the manufacturer's instructions. Properdin was depleted from human serum and plasma by recirculation 3 ml over the mAb 1340 column for 2 h. Through flow was tested in the reported sandwich ELISA and Western blot for properdin concentration. There was no properdin detectable.
Pauly D., Nagel B.M., Reinders J., Killian T., Wulf M., Ackermann S., Ehrenstein B., Zipfel P.F., Skerka C, & Weber B.H. (2014). A Novel Antibody against Human Properdin Inhibits the Alternative Complement System and Specifically Detects Properdin from Blood Samples. PLoS ONE, 9(5), e96371.
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