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Anti acetylated monoclonal antibody

Manufactured by Merck Group
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The anti-acetylated monoclonal antibody is a laboratory reagent used to detect and quantify acetylated proteins. It is a highly specific and sensitive tool for researchers studying post-translational modifications and their role in cellular processes.

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Lab products found in correlation

Ab6161, by Abcam (2 mentions) Clone 6 11b 1, by Merck Group (1 mentions)

Spelling variants of this product

Mouse monoclonal anti-acetylated tubulin antibody (15 citations) Monoclonal anti-acetylated tubulin antibody (4 citations) Mouse anti-acetylated α-tubulin monoclonal antibody (2 citations) Monoclonal anti-acetylated tubulin antibody (clone 6–11B-1, (2 citations)

2 protocols using anti acetylated monoclonal antibody

1

Microtubule Immunostaining Protocol

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For microtubule staining, cells were fixed in formaldehyde (5 min) followed by 10 min permeabilization with 0.01% TritonX-100, 1% BSA in PBS, and blocking in 1% BSA in PBS. Abcam antibody ab6161 was used for immunostaining of α-tubulin. Acetylated tubulins were stained with anti-acetylated monoclonal antibody from Sigma (clone 6-11B-1, cat # T7451). Immunostained cells were imaged by confocal microscopy, with constant parameters for excitation, emission, pinhole, exposure time when levels of immunostained material were compared.
Chauhan S., Ahmed Z., Bradfute S.B., Arko-Mensah J., Mandell M.A., Won Choi S., Kimura T., Blanchet F., Waller A., Mudd M.H., Jiang S., Sklar L., Timmins G.S., Maphis N., Bhaskar K., Piguet V, & Deretic V. (2015). Pharmaceutical screen identifies novel target processes for activation of autophagy with a broad translational potential. Nature Communications, 6, 8620.
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2

Immunostaining Microtubules in Cells

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For microtubule staining, cells were fixed in formaldehyde (5 min) followed by 10 min permeabilization with 0.01% TritonX-100, 1% BSA in phosphate-buffered saline (PBS) and blocking in 1% BSA in PBS. Abcam antibody ab6161 was used for immunostaining of α-tubulin. Acetylated tubulins were stained with anti-acetylated monoclonal antibody from Sigma (clone 6-11B-1, cat # T7451). Immunostained cells were imaged by confocal microscopy, with constant parameters for excitation, emission, pinhole and exposure time when levels of immunostained material were compared.
Chauhan S., Ahmed Z., Bradfute S.B., Arko-Mensah J., Mandell M.A., Won Choi S., Kimura T., Blanchet F., Waller A., Mudd M.H., Jiang S., Sklar L., Timmins G.S., Maphis N., Bhaskar K., Piguet V, & Deretic V. (2015). Pharmaceutical screen identifies novel target processes for activation of autophagy with a broad translational potential. Nature Communications, 6, 8620.
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