R26 rtta

Animal care and experiments were approved by the Institutional Animal Care and Use Committee at University of Texas Southwestern Medical Center. Scf^flox and Scf^gfp were generated in a previous report (Ding et al. 2012 (link)). K14Cre (Dassule et al. 2000 (link)), K14Cre^ERT (Vasioukhin et al. 1999 (link)), TyrCre^ERT2 (Bosenberg et al. 2006 (link)), R26-rtTA (Belteki et al. 2005 (link)), and tetO-DTA (Lee et al. 1998 (link)) mice were purchased from the Jackson Laboratory. K14-Scf (Kunisada et al. 1998a (link)) was kindly provided by Dr. John Harris (University of Massachusetts Medical School). Krox20Cre (Voiculescu et al. 2000 (link)), DhhCre (Skucas et al. 2013 (link)), PLPCre^ERT2 (Leone et al. 2003 (link)), CMVCre^ERT (Hayashi and McMahon 2002 (link)), R26-LacZ, and R26-YFP were described previously (Le et al. 2009 (link); Chen et al. 2014 (link)).

All mice were housed and cared for according to MSSM and IACUC approved protocols. Ezh1^deleted and Ezh2^flox mice were previously reported [45 (link)]. EED^flox mice were provided by Weipeng Mu and Terry Magnuson [70 (link)]. Tcf/Lef:H2B-GFP mice were provided by Anna-Katerina Hadjantonakis [36 (link)]. Gli1^LacZ, Ctnnb1^flox, Smo^flox, Shh^EGFPCre, Atoh1-GFP, R26-rtTA, and Krt14-Cre mice were obtained from The Jackson Laboratory. Wild type C57BL/6 mice were obtained from Charles River Laboratories. Mice were genotyped by PCR using DNA extracted from tail skin. BrdU was administered as previously reported [51 (link)]. Briefly, BrdU was administered (50μg BrdU per 1g mouse weight) to mice or pregnant females 3–5 h before sacrificing [45 (link)].