Hepatocytes plated onto glass coverslips were subjected to fasted and fed conditions as indicated above and fixed for 20 min with 4% formaldehyde. The cells were made permeable with 0.1% Triton X-100 for 2 min, washed with PBS, and then stained with BODIPY (1 µg /ml) in PBS for 20 min at room temperature, followed by three washes with PBS. Coverslips were mounted onto glass slides using a DAPI mounting media for nuclear stain (Vector Laboratories, Burlingame, CA). Images were acquired using a Zeiss 510 Meta Confocal Laser Scanning Microscope (Carl Zeiss, Thornwood, NY). Quantifications of LD number and size were carried out using ImageJ software (NIH, Bethesda, MA). For quantification, 5 different fields were randomly selected from each coverslip and data derived were pooled from samples obtained from four sets of control and EtOH-fed animal pairs.
Dapi mounting media for nuclear stain
Manufactured by Vector Laboratories
Sourced in Canada
DAPI mounting media is a fluorescent dye used to stain nuclei of cells. It binds to DNA and emits blue fluorescence when excited by ultraviolet light. The media aids in preserving the fluorescent signal during microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dapi mounting media for nuclear stain
1
Quantifying Lipid Droplet Dynamics in Fasted and Fed Hepatocytes
2
Quantifying Hepatocyte Lipid Droplets
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Hepatocytes plated onto glass coverslips were subjected to fasted and fed conditions as indicated above and fixed for 20 minutes with 4% formaldehyde. The cells were made permeable with 0.1% Triton X‐100 for 2 minutes, washed with PBS, and then stained with BODIPY (1 μg/mL) in PBS for 20 minutes at room temperature, followed by three washes with PBS. Coverslips were mounted onto glass slides using a DAPI mounting media for nuclear stain (Vector Laboratories, Burlingame, CA). Images were acquired using a Zeiss 510 Meta Confocal Laser Scanning Microscope (Carl Zeiss, Thornwood, NY). Quantifications of LD number and size were performed using ImageJ software (National Institutes of Health, Bethesda, MA). For quantification, five different fields were randomly selected from each coverslip and data derived were pooled from samples obtained from four sets of control and EtOH‐fed animal pairs.
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