Rt2 microrna first strand kit

Manufactured by Qiagen

The RT2 microRNA First Strand Kit is a laboratory tool designed for the reverse transcription of microRNA molecules. It provides a standardized and efficient method for the conversion of microRNA into complementary DNA (cDNA) that can be used in subsequent qPCR or other molecular analysis applications.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Pcr focused immunopathology microrna array plates, by Qiagen (2 mentions) Mirneasy mini kit, by Qiagen (1 mentions) Cfx96 connect real time pcr system, by Bio-Rad (1 mentions)

Close spelling variants of this product

RT2 First Strand Kit (1647 citations)

3 protocols using rt2 microrna first strand kit

Comprehensive Mouse microRNA Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

For microRNA analysis, total RNA, including microRNA, was extracted from cell lysates using a miRNeasy Mini Kit (Qiagen cat#217004). Next, the RT² microRNA First Strand Kit (Qiagen cat# 331401) was used to synthesize cDNA from 4 µg of total RNA. The microRNA PCR array (Mouse miRNome RT² Whole Genome microRNA PCR Array, Qiagen cat#MAM-200A-2) was used to probe for 440 mouse microRNAs normalized to the average levels of the housekeeping small nuclear RNAs, SNORD 68, 66 and Rnu6.

Khosravi R., Sodek K.L., Xu W.P., Bais M.V., Saxena D., Faibish M, & Trackman P.C. (2014). A Novel Function for Lysyl Oxidase in Pluripotent Mesenchymal Cell Proliferation and Relevance to Inflammation-Associated Osteopenia. PLoS ONE, 9(6), e100669.

+ Open protocol

+ Expand

Inflammatory microRNA Expression in Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the expression of inflammatory microRNAs, WT and 5-LO−/− macrophages were treated with or without LTB₄ for 24 h. RNA was then extracted from the cells using a Qiagen RNeasy mini kit according to the manufacturer’s instructions. cDNA was synthesized using the RT2 microRNA First Strand Kit (Qiagen) and applied to PCR focused immunopathology microRNA array plates (Qiagen). Plates were processed in a BioRad CFX96 Connect Real-Time PCR System using automated baseline and threshold cycle detection.

Wang Z., Filgueiras L., Wang S., Serezani A.P., Peters-Golden M., Jancar S, & Serezani C.H. (2014). Leukotriene B4 enhances the generation of pro-inflammatory microRNAs to promote MyD88-dependent macrophage activation. Journal of immunology (Baltimore, Md. : 1950), 192(5), 2349-2356.

+ Open protocol

+ Expand

Profiling Inflammatory microRNAs in Macrophages

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the expression of inflammatory microRNAs, macrophages from C57BL/6 WT mice were treated with PTEN siRNA or scrambled siRNA as above. RNA was then extracted from the cells using Qiagen RNeasy mini kit according to the manufacturer’s instructions. cDNA was synthesized using the RT2 microRNA First Strand Kit (Qiagen) and applied to PCR focused immunopathology microRNA array plates (Qiagen) as previously shown (10 (link)). Normalization and statistical analysis of miRNA expression was conducted using SABiosciences’ Online PCR Array Data Analysis Web Portal as previously described (10 (link)).

Sisti F., Wang S., Brandt S.L., Glosson-Byers N., Mayo L., Son Y.M., Sturgeon S., Filgueiras L., Jancar S., Wong H., Dela Cruz C.S., Andrews N., Alves-Filho J.C., Cunha F.Q, & Serezani C.H. (2018). Nuclear PTEN enhances the maturation of a microRNA regulon to limit MyD88-dependent susceptibility to sepsis. Science signaling, 11(528), eaai9085.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!