Ai 4001

Manufactured by Vector Laboratories

Sourced in United States

The AI-4001 is a multifunctional laboratory instrument designed for various analytical applications. It features automated sample handling, data analysis, and reporting capabilities. The core function of the AI-4001 is to provide researchers with a versatile and efficient platform for their laboratory workflows.

Automatically generated - may contain errors

Lab products found in correlation

Vector red substrate, by Vector Laboratories (2 mentions) Scn400 slide scanner, by Leica (2 mentions) Sc 130917, by Santa Cruz Biotechnology (1 mentions) K4011, by Agilent Technologies (1 mentions) Sk 4805, by Vector Laboratories (1 mentions) Tween 20, by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Envision k4003, by Agilent Technologies (1 mentions) Tissueia software, by Leica (1 mentions) Alexa fluor 546, by Thermo Fisher Scientific (1 mentions) Ep3070, by Abcam (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) I2018, by Merck Group (1 mentions) Hoechst 33258, by Merck Group (1 mentions) Dia 310, by Dianova (1 mentions) Digital image hub software, by Leica (1 mentions) Dako envision kit, by Agilent Technologies (1 mentions)

6 protocols using ai 4001

Quantification of CD8+ T Cells in 4T1 Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Paraffin-embedded sections from 4T1 tumors were cut at 4 μm thickness and paraffin removed with xylene and rehydrated through graded ethanol. Endogenous peroxidase activity was blocked with 3% hydrogen peroxide in methanol for 10 min. Heat-induced antigen retrieval (HIER) was carried out for all sections in 0.001M EDTA buffer, pH = 8.00 using a vegetable steamer at 95°C for 25 min. Sections were incubated with anti mouse CD8a (eBioscience, 14–0808-82) at 1:100 dilution for 1 hour at room temperature. After primary antibody incubation, tissues were then incubated with secondary rabbit anti-rat immunoglobulin for 30 min at 1:200 dilution (Vector, AI-4001) followed by a 30 min incubation with Dakocytomation EnvisionÅ System Labelled Polymer HRP anti rabbit (Agilent, K4003). All sections were visualized with the diaminobenzidine reaction and counterstained with hematoxylin. The number of immune-positive cells were counted in five randomly chosen fields per tumor at 100-fold magnification. 4–6 mice tumors per condition were used for analysis. Results from the five areas/mouse were averaged and used in the statistical analysis.

Márquez-Garbán D.C., Deng G., Comin-Anduix B., Garcia A.J., Xing Y., Chen H.W., Cheung-Lau G., Hamilton N., Jung M.E, & Pietras R.J. (2019). Antiestrogens in combination with immune checkpoint inhibitors in breast cancer immunotherapy. The Journal of steroid biochemistry and molecular biology, 193, 105415.

+ Open protocol

+ Expand

Whole-mount in situ hybridization and Blimp1 immunohistochemistry

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Whole-mount in situ hybridisation analysis was performed as before^{50 (link)}, using antisense riboprobes for Bmp2^{64 (link)}, Bmp4^{13 (link)} and Gdf3^{28 (link)}. For Blimp1 immunohistochemistry, E7.5 decidua were fixed overnight in 4% PFA, dehydrated in ethanol, embedded in paraffin wax and sectioned (6 μm). Dewaxed sections were subjected to antigen retrieval by boiling for 1 h in Tris/EDTA (pH 9.0) and permeabilized for 10 min in 0.1% Triton X-100 in TBS. Sections were subsequently blocked with 10% normal goat serum in TBS. Sections were incubated in rat monoclonal anti-Blimp1 (1:500 dilution, sc-130917, Santa Cruz Biotechnology) in block overnight at 4 °C and signal-amplified with rabbit anti-rat secondary antibody (AI-4001, Vector Laboratories) for 45 min at RT followed by peroxidase blocking for 20 min at RT and development with Envison System-HRP for rabbit antibodies (K4011, DAKO) and Vector Red substrate (SK-4805, Vector Laboratories). Sections were lightly counter stained with haematoxylin, coverslipped and imaged.
Haematoxylin and eosin staining was performed as previously described^{36 (link)}.

Senft A.D., Bikoff E.K., Robertson E.J, & Costello I. (2019). Genetic dissection of Nodal and Bmp signalling requirements during primordial germ cell development in mouse. Nature Communications, 10, 1089.

+ Open protocol

+ Expand

Immunohistochemistry of Uterine Development

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunohistochemistry (IHC), virgin and pregnant (E3.5, E4.5, E5.5 and E6.5) uteri or individual decidua were fixed overnight in 4% paraformaldehyde (PFA) in PBS, dehydrated using an ethanol series, embedded in paraffin wax and sectioned (6 μm). Dewaxed sections were subjected to antigen retrieval by boiling for 1 h in Tris/EDTA (pH 9.0) and permeabilised for 10 min in 0.1% Triton X-100 (Sigma) in PBS. After blocking with 10% normal goat serum in PBS with 0.05% Tween-20 (Sigma) for 1 h at RT, sections were incubated with primary antibodies in blocking solution overnight at 4 °C. Rat monoclonal antibodies underwent signal amplification with rabbit anti-rat secondary antibody (AI-4001, Vector Laboratories) for 45 min at RT. All samples were then subjected to peroxidase blocking for 20 min at RT and developed with Envison System-HRP for rabbit antibodies (K4011, DAKO) and Vector Red substrate (SK-4805, Vector Laboratories). Sections were lightly counterstained with haematoxylin, coverslipped and imaged. Haematoxylin and eosin staining was performed as per standard protocols. Antibodies used are listed in the Supplementary Table 1.

Goolam M., Xypolita M.E., Costello I., Lydon J.P., DeMayo F.J., Bikoff E.K., Robertson E.J, & Mould A.W. (2020). The transcriptional repressor Blimp1/PRDM1 regulates the maternal decidual response in mice. Nature Communications, 11, 2782.

+ Open protocol

+ Expand

Quantification of Liver Fibrosis and Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin‐fixed and paraffin‐embedded tissue was cut in 5‐μm‐thick sections. Each macroscopic tumor lesion was microscopically analyzed by H&E staining to confirm the tumor diagnosis.
Liver fibrosis quantification on non‐tumor parenchyma (percentage of stained area in the section) was carried out on Sirius red‐stained tissue using Tissue IA software (Leica Biosystems, Dublin, Ireland) after digitalization with a SCN400 slide scanner (Leica Biosystems, Wetzlar, Germany).
Macrophages were identified by F4/80 immunostaining using a primary rat anti‐mouse F4/80 monoclonal Ab (1:200, MCA497G, Clone A3‐1; AbD Serotec, Oxford, UK), a rabbit anti‐rat immunoglobulin (1:100, AI‐4001; Vector Laboratories, Burlingame, CA, USA), and then a goat anti‐rabbit streptavidin HRP‐conjugated Ab (En Vision K4003; Dako, Glostrup, Denmark). Peroxidase activity was seen with diaminobenzidine (DAB) and slides counterstained with hematoxylin.

Delire B., Henriet P., Lemoine P., Leclercq I.A, & Stärkel P. (2018). Chronic liver injury promotes hepatocarcinoma cell seeding and growth, associated with infiltration by macrophages. Cancer Science, 109(7), 2141-2152.

+ Open protocol

+ Expand

Comprehensive Histological and Immunochemical Evaluation of Pancreatic Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Histological and immunochemical reactions were performed on formalin-fixed paraffin embedded sections. Briefly, mouse pancreata were excised (n=4–12 per treatment group), fixed overnight in 10% neutral-buffered formalin, paraffin-embedded and serially sectioned at 4 μm. Multiple sections (n=3–6) from each pancreas were cut 50–100 microns in depth from one another and were processed using a BOND-MAX automatic IHC system (Leica, Buffalo Grove, IL, USA) using heat-mediated EDTA antigen retrieval (100°C for 20 minutes). Sections were stained with anti-CD31 (DIA-310 Dianova, Hamburg, Germany, diluted 1:250) followed by rabbit anti-rat secondary (AI-4001 Vector Laboratories, Burlingame, CA, USA 1:300). Leica Bond Mixed Refine reagents (HRP and 3,3’-diaminobenzidene-based) were used for detection and slides were counterstained with hematoxylin. Isotype-matched IgG antibodies were included as negative controls.
For insulin and glucagon immunofluorescence, serial sections from each experimental group underwent immunohistochemistry with antibodies against either insulin (I2018 Sigma 1:2000), or glucagon (EP3070 Abcam 1:2000) followed by AlexaFluor 488 and AlexaFluor 546 secondary antibodies, respectively (Life Technologies 1:200). Nuclei were stained with Hoechst 33258 (Sigma 2 μg/ml).

Hogan M.F., Hackney D.J., Aplin A.C., Mundinger T.O., Larmore M.J., Castillo J.J., Esser N., Zraika S, & Hull R.L. (2021). SGLT2-i improves markers of islet endothelial cell function in db/db diabetic mice. The Journal of endocrinology, 248(2), 95-106.

+ Open protocol

+ Expand

Histological Analysis of Adipose Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Subcutaneous adipose tissue depots were fixed in 4% paraformaldehyde for 24 h at room temperature. Samples were then immersed in ethanol 100% for 24 h before processing for paraffin embedding.
To determine the adipocyte tissue diameter, paraffin sections of 8 m were stained with hematoxylin and eosin. Macrophage infiltration in the adipose tissue was assessed by staining them with a MAC-2/galectin-3 antibody (CL8942AP, Cedarlane Laboratories, Burlington, ON) diluted 1:500 in blocking buffer overnight, and was detected with an anti-rat igG antibody (AI-4001, Vector Laboratories, Burlingame, CA; 10 mg/ml). Immune complexes were detected by a Dako Envision kit (Agilent Technologies, Santa Clara, CA), according to the manufacturer's instructions, and briefly counterstained with hematoxylin. Hepatic lipid content was visualized by using Oil red O staining.
All analyses were performed in a blinded manner by the investigator and quantified using ImageJ software (version 1.50a, National Institutes of Health, Bethesda, MD). At least five high-magnification fields were selected at random for each mouse. Images were obtained using a SCN400 slide scanner and Digital Image Hub software (Leica Biosystems, Wetzlar, Germany).

Van Hul M., Geurts L., Plovier H., Druart C., Everard A., Ståhlman M., Rhimi M., Chira K., Teissedre P.L., Delzenne N.M., Maguin E., Guilbot A., Brochot A., Gérard P., Bäckhed F, & Cani P.D. (2018). Reduced obesity, diabetes, and steatosis upon cinnamon and grape pomace are associated with changes in gut microbiota and markers of gut barrier. American journal of physiology. Endocrinology and metabolism, 314(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!