Mouse plasma was collected from the tail vein routinely, and from the vena cava at the end point. ALT and AST were measured using Transaminase CII-test Wako (Wako Pure Chemical Industries, Osaka, Japan), according to the manufacture’s instruction for 96-well format. Plasma ALT and AST levels are shown in international unit.
Transaminase cii test wako
Manufactured by Fujifilm
Sourced in Japan
The Transaminase CII-test Wako is a laboratory equipment product designed for the quantitative determination of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in human serum or plasma samples. It is a diagnostic tool used to assess liver function.
Automatically generated - may contain errors
Lab products found in correlation
Glucose cii test wako, by Fujifilm (3 mentions)
Quantichrom bilirubin assay kit, by BioAssay Systems (2 mentions)
Calcium e test, by Fujifilm (2 mentions)
Bun wako test, by Fujifilm (2 mentions)
Cholesterol e test wako, by Fujifilm (2 mentions)
Labassay alp, by Fujifilm (2 mentions)
Labassay creatinine, by Fujifilm (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Sepasol rna 1 super g, by Nacalai Tesque (1 mentions)
Dulbecco s modified eagle s medium, by Nacalai Tesque (1 mentions)
Fast sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Lps from escherichia coli, by Merck Group (1 mentions)
Glutathione (gsh), by Nacalai Tesque (1 mentions)
Ms grade porcine pancreatic trypsin, by Fujifilm (1 mentions)
Fps zm1, by Cayman Chemical (1 mentions)
Revertra ace, by Toyobo (1 mentions)
Percoll, by Cytiva (1 mentions)
Soybean oil, by Fujifilm (1 mentions)
Casein, by Oriental Yeast (1 mentions)
Cellulose, by Oriental Yeast (1 mentions)
30 protocols using transaminase cii test wako
1
Plasma Transaminase Measurement in Mice
2
Hepatocyte Isolation and Characterization
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GSH, GSSG, Sepasol-RNA I Super G, and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Nacalai Tesque Inc. (Kyoto, Japan). ReverTra Ace was procured from Toyobo Co., Ltd. (Osaka, Japan). Fast SYBR Green Master Mix and the BCA protein assay kit were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Transaminase CII-test Wako and MS-grade porcine pancreatic trypsin were obtained from Fujifilm Wako Pure Chemical Co., Ltd. (Osaka, Japan). The QuantiChrom bilirubin assay kit was obtained from BioAssay Systems, LLC. (Hayward, CA, USA). Percoll was obtained from Cytiva (Tokyo, Japan). LPS from Escherichia coli was procured from Sigma-Aldrich Co., LLC. (St. Louis, MO, USA). LPS-RS was obtained from InvivoGen Inc. (San Diego, CA, USA). FPS-ZM1 was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). Oligonucleotide primers were obtained from Eurofins Genomics Inc. (Luxembourg, Luxembourg). All other chemicals and solvents were of MS grade or higher and of commercially available purity.
3
Plasma Biochemical Analysis Protocol
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Plasma calcium levels were measured using the calcium-E test (Wako Chemical) according to the manufacturer’s instructions. Plasma sample (2.5 μL) was mixed with substrate buffer (100 μL) and coloring reagent (50 μL). The absorbance of the reaction mixture was measured at 610 nm.
Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured using the Transaminase CII Test Wako (Wako Chemical) according to the manufacturer’s instructions and as previously described [20 (link), 21 (link)]. Concentrations of plasma creatinine and blood urea nitrogen (BUN) were measured using Creatinine Liquid Reagents Assay (DIAZYME, Poway, CA) and BUN Wako Test (Wako Chemical), respectively, according to the manufacturer’s instructions and as previously described [22 (link), 23 (link)]. For relative quantification, calibration curves were prepared using standard solutions.
Plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured using the Transaminase CII Test Wako (Wako Chemical) according to the manufacturer’s instructions and as previously described [20 (link), 21 (link)]. Concentrations of plasma creatinine and blood urea nitrogen (BUN) were measured using Creatinine Liquid Reagents Assay (DIAZYME, Poway, CA) and BUN Wako Test (Wako Chemical), respectively, according to the manufacturer’s instructions and as previously described [22 (link), 23 (link)]. For relative quantification, calibration curves were prepared using standard solutions.
4
Analysis of Blacklip Abalone Compounds
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Viscera were obtained from Australian Blacklip abalone (Haliotis rubra) cultured in Australia, frozen, and shipped. d (+)-Glucose, Na2HPO4, KH2PO4, NaOH, soybean oil, tert-butylhydroquinone, NaCl, isoflurane, glucose CII-Test Wako, TG E-Test Wako, cholesterol E-Test Wako, high-density lipoprotein (HDL)-cholesterol E-Test Wako, and transaminase CII-Test Wako were purchased from Fujifilm Wako Pure Chemical Co. (Osaka, Japan). D2O was purchased from Kanto Chemical Co. (Tokyo, Japan), and the ACE-kit WST was purchased from Dojin Molecular Technology, Inc. (Kumamoto, Japan). Plate count agar with bromocresol purple was purchased from Nissui Pharmaceutical Co. (Tokyo, Japan), and acetonitrile was purchased from Sigma-Aldrich (St. Louis, MO, USA). β-Corn starch, casein, α-corn starch, sucrose, cellulose, AIN76 mineral mixture, AIN76A vitamin mixture, and l -cysteine were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan).
5
Serum Aminotransferase and Bilirubin Measurement
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Serum aminotransferases were measured by Transaminase CII-test Wako (Wako Pure Chemical Industries Ltd.) according to the manufacture’s protocol. Serum bilirubin was measured by QuantiChrom™ Bilirubin Assay Kit (BioAssay Systems LLC, Hayward, CA). Both absorbance was measured using plate reader (Tecan Japan Co., Ltd., Kanagawa, Japan).
6
Liver Injury Biochemical Markers Assay
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Liver injury was estimated by biochemical blood markers AST and ALT. Blood samples were
collected from heart with heparin-treated needle and syringe, and then centrifuged at ×
10,000 g for 10 min at 4°C to separate plasma fraction. Measurement of AST and ALT in
plasma sample was performed using Transaminase C-II-test WAKO (Wako Pure Chemical, Osaka,
Japan) according to the manufacturer’s instructions with brief modifications.
collected from heart with heparin-treated needle and syringe, and then centrifuged at ×
10,000 g for 10 min at 4°C to separate plasma fraction. Measurement of AST and ALT in
plasma sample was performed using Transaminase C-II-test WAKO (Wako Pure Chemical, Osaka,
Japan) according to the manufacturer’s instructions with brief modifications.
7
Con A-Induced Hepatitis Protocol
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Con A-induced hepatitis was generated as described elsewhere27 (link). Fourteen hours after the injection of Con A, sera were collected, and the levels of GOT and GPT in the sera were determined using Transaminase CII-test Wako (Wako).
8
Transaminase Measurement for Liver Injury
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The serum liver injury markers, ALT and AST were measured using Transaminase C-II Test Wako (Wako, Japan) according to the manufacturer’s instruction.
9
Biomarker Assays for Metabolic Health
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Adiponectin, IgA, and glucose were analyzed using Mouse/Rat Adiponectin ELISA Kit (Otsuka Pharmaceutical Co. Ltd., Tokyo, Japan), Rat IgA ELISA kit (Bethyl Laboratories Inc., Montgomery, TX, USA), and Glucose CII-test Wako (Wako Pure Chemical Industries Ltd., Osaka, Japan), respectively. Transaminase CII-test Wako (Wako Pure Chemical Industries) was used to determine the aspartate aminotransferase (AST)/alanine aminotransferase (ALT) activities.
10
Effect of NEK6 Knockdown on Hepatic Disorder
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Example 15
Fibrogenesis has been understood as an excessive wound healing process against disorder of a cell or tissue. Thus, suppression of disorder of a cell or tissue along with fibrogenesis has been considered to be effective for treatment of various fibrosis. Then, in order to investigate whether NEK6 knockdown would exhibit effect on hepatic disorder, measurement of hepatic disorder markers in CCl4 models was performed.
On day 13 after induction of pathology, blood draw was performed from tail vein using a plane capillary blood-sampling tube, and subjected to standing for 30 minutes or more. The post-standing blood was centrifuged to obtain serum. Serum glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT) were measured using Transaminase CII-test Wako (Wako Pure Chemical Industries, Ltd.). Measurement method followed the instruction of the reagent.
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