All fluorescence spectra of the Cav1-TDD system were collected on the F-4700 fluorescence spectrometer (Hitachi, Japan). Working voltage and scanning speed were controlled at 700 V and 240 nm/min. The Cav1 concentration was kept at 5.0 × 10−9 mol/L, while varying the TDD concentration (0, 1.0 × 10−7, 2.0 × 10−7, 3.0 × 10−7, 4.0 × 10−7 and 5.0 × 10−7 mol/L) at 298 K and 310 K. The excitation wavelength was set at 280 nm and the emission wavelength was performed from 300 nm to 420 nm. The excitation and emission slit widths were fixed at 5 nm.
The three-dimensional (3D) fluorescence spectra of Cav1 (5.0 × 10−9 mol/L) were recorded at 298 K in the absence and presence of TDD (5.0 × 10−7 mol/L) was measured on the F-4700 fluorescence spectrometer (Hitachi, Japan). The emission wavelength was performed between 300 nm and 420 nm with 5 nm interval. The excitation wavelength was recorded between 200 nm and 300 nm with 5 nm interval.
The three-dimensional (3D) fluorescence spectra of Cav1 (5.0 × 10−9 mol/L) were recorded at 298 K in the absence and presence of TDD (5.0 × 10−7 mol/L) was measured on the F-4700 fluorescence spectrometer (Hitachi, Japan). The emission wavelength was performed between 300 nm and 420 nm with 5 nm interval. The excitation wavelength was recorded between 200 nm and 300 nm with 5 nm interval.