Uqcrq

Manufactured by Cell Signaling Technology

UQCRQ is a protein that is part of the mitochondrial respiratory complex III, also known as the cytochrome bc1 complex. It plays a core role in the electron transport chain, which is essential for cellular energy production.

Automatically generated - may contain errors

Lab products found in correlation

Eif4e, by Cell Signaling Technology (1 mentions) Immobilon fl transfer membrane, by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Pcbp2, by Cell Signaling Technology (1 mentions) Cox5a, by Cell Signaling Technology (1 mentions) Mcl 1, by Thermo Fisher Scientific (1 mentions) Aldoa, by Thermo Fisher Scientific (1 mentions) Hmgcs1, by Thermo Fisher Scientific (1 mentions) Bcl 2, by Thermo Fisher Scientific (1 mentions) Gapdh d16h11, by Cell Signaling Technology (1 mentions)

2 protocols using uqcrq

Protein Expression Analysis Protocol

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Lysates were made using TNN lysis buffer (50 mM Tris-Cl, 250 mM NaCl, 5 mM EDTA, and 0.5% NP-40 supplemented with protease inhibitor). 60 μg of protein was loaded onto SDS-PAGE gels and then transferred onto Immobilon-FL transfer membranes (IPFL00010; Millipore). The antibodies used were MYC, SRSF1, RBM42, PCBP2, UQCRQ, ATP6V0B, COX5A, EIF4E, AKT1, and NDUFA1 (Cell Signaling Technology) and β-actin (A5316; Sigma-Aldrich).

Singh K., Lin J., Zhong Y., Burčul A., Mohan P., Jiang M., Sun L., Yong-Gonzalez V., Viale A., Cross J.R., Hendrickson R.C., Rätsch G., Ouyang Z, & Wendel H.G. (2019). c-MYC regulates mRNA translation efficiency and start-site selection in lymphoma. The Journal of Experimental Medicine, 216(7), 1509-1524.

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Quantitative Proteomic and Transcriptomic Analysis of Metabolic Pathways

Cited 1 time

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Western blotting and qPCR were conducted as previously described (59 (link)). Anti-CDK7 (MO1), CDK9 (C12F7), PRMT5 (D5P2T), DHODH (E9X8R), adolase A (D73H4), hexokinase I (C35C4), hexokinase II (C64G5), PFKFB3 (D7H4Q), enolase-1, enolase-2 (E2H9X), PKM1 (D30G6), PKM2 (D78A4), LDHA (C4B5), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (D16H11), PFAS, SDHA, UQCRQ, and GMPS antibodies were purchased from Cell Signaling Technologies (Danvers, MA). GART and PAICS antibodies were obtained from Abcam. TaqMan primers for PRMT5 (Hs01047345), DHODH (Hs00361406), CDK9 (Hs00977896), CDK7 (Hs00361486), BCL2 (Hs00608023), MCL1 (Hs06626047_g1), Myc (Hs00905030), ALDOA (Aldolase A) (Hs00605108), ENO1 (enolase 1) (Hs00361415), PKM (Hs00761782), FH (Hs00264683), UQCRQ (Hs00429571), GART (Hs00894582), PAICS (Hs00935017), FDFT1(Hs00926054), HMGCS1 (Hs00940429), PTK2 (Hs01056457), KRT18 (keratin 18) (Hs01920599), SLC38A2 (Hs01089954), and HK1 (Hs00175976) were obtained from Thermo Fisher Scientific (Waltham, MA).

Zhang P., Brinton L.T., Gharghabi M., Sher S., Williams K., Cannon M., Walker J.S., Canfield D., Beaver L., Cempre C.B., Phillips H., Chen X., Yan P., Lehman A., Scherle P., Wang M., Vaddi K., Baiocchi R., Wang R., Sampath D., Alinari L., Blachly J.S, & Lapalombella R. (2022). Targeting OXPHOS de novo purine synthesis as the nexus of FLT3 inhibitor–mediated synergistic antileukemic actions. Science Advances, 8(37), eabp9005.

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