Microscopic glass slides and potassium dichromate were obtained from HiMedia (Mumbai, India). Hydrofluoric acid (HF), sulphuric acid, ammonia and glutaraldehyde (GA) were obtained from Merck Millipore (Bengaluru, India). 3′-Aminopropyl triethoxysilane (APTES) was obtained from Sigma Aldrich (USA). Tetra methylene benzidine–hydrogen peroxide (TMB/H2O2) was obtained from Aristogene Pvt Ltd (Bengaluru, India). Freund's complete and incomplete adjuvant, horse anti-rabbit IgG HRP and donkey anti-chicken IgY HRP were procured from Sigma Aldrich (US). Other chemicals used in the study were fine grade and obtained from Merck Millipore (Bengaluru, India).
Freund s complete and incomplete adjuvant
Manufactured by Merck Group
Sourced in United States
Freund's complete and incomplete adjuvants are laboratory reagents used in immunological research. Complete adjuvant contains killed Mycobacterium tuberculosis, while incomplete adjuvant does not contain the mycobacterial component. These adjuvants are used to enhance the immune response to antigens in experimental immunization protocols.
Automatically generated - may contain errors
Lab products found in correlation
Tween 20, by Merck Group (6 mentions)
3 3 5 5 tetramethylbenzidine (tmb), by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Protein a agarose, by Merck Group (3 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
N hydroxysuccinimide, by Merck Group (3 mentions)
Hypoxanthine aminopterin thymidine, by Merck Group (3 mentions)
Bicinchoninic acid bca protein estimation kit, by Merck Group (2 mentions)
Sepharose 4b, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Poly ethylene glycol peg 1500, by Merck Group (2 mentions)
P nitrophenyl phosphate, by Merck Group (2 mentions)
Balb c mice, by Charles River Laboratories (2 mentions)
Keyhole limpet hemocyanin (klh), by Merck Group (2 mentions)
L glutamine, by Merck Group (2 mentions)
Thymidine, by Merck Group (2 mentions)
Chloroquine, by Merck Group (2 mentions)
Hypoxanthine, by Merck Group (2 mentions)
Aminopterin, by Merck Group (2 mentions)
36 protocols using freund s complete and incomplete adjuvant
1
Fabrication of Silane-Functionalized Microscopic Slides
2
Listeria Immunoassay Development Protocol
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All the chemicals and reagents used were of the highest purity available. Phenylmethanesulfonylfluoride, β–mercaptoethanesulfonic acid sodium salt, β–mercaptoethanol, Ethanolamine, Sepharose-4B, Freund's complete and incomplete adjuvant, Bicinchoninic acid (BCA) protein estimation kit, Tween-20 and FITC conjugated mouse anti-rabbit antibody were purchased from Sigma-Aldrich Chemicals (St Louis, MO) and used as received. Horseradish peroxidase-conjugated anti-rabbit IgG were purchased from Bangalore Genei (India) Pvt. Ltd. (Bangalore, India). Mouse monoclonal anti-Listeria LZH1 IgG1 and mouse monoclonal IgG2a specific for the human erythrocyte membrane protein (Protein4.2 (2G-12)) were procured from Santa Cruz Biotechnology, Inc., California and Abnova Corporation respectively.
3
Polyclonal Antibody Production for Dairy Proteins
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Casein (purity > 85%), ɑ‐LA (purity > 85%), β‐LG (purity > 85%), alkaline phosphatase‐conjugated goat anti‐rabbit IgG antibodies, Freund's complete and incomplete adjuvant, o‐phthalaldehyde (OPA), dithiothreitol (DTT), o‐phenylenediamine dihydrochloride (OPD), Papain and Pepsin were purchased from Sigma‐Aldrich. Alcalase, Neutrase, Protamex, and Flavourzyme were purchased from Novozymes. Three types of rabbit serum comprising polyclonal antibodies targeting CN, ɑ‐LA, β‐LG were prepared at the Shenyang Agricultural University.
4
Antibody Production and Characterization
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The antibody of TrxBEp was prepared as described previously [30 (link)]. Freund’s complete and incomplete adjuvant were purchased from Sigma-Aldrich. The detection of antibody titer was determined by indirect ELISA [32 (link)].
5
Preparation of Formalin-Inactivated Pasteurella multocida Vaccines
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Scaled up culture from seed culture of P. multocida isolate was prepared in BHI broth. Formalin inactivated vaccine was prepared as per the method described by Jones et al. (1988) . Briefly, formaldehyde was added to bacterial culture harvested from bulk broth culture from 1 liter of 18h culture, washed and resuspended in 20 ml of normal saline up to the final concentration of 0.5%. After incubating for 24h at 37°C, the inactivated culture was washed and resuspended in normal saline. This was adjuvanted with 0.5% aluminum potassium sulfate (Merck) for the preparation of formalin inactivated with aluminum potassium sulfate vaccine (FIA). The formalin inactivated antigen was combined with Freund's complete and incomplete adjuvant (Sigma-Aldrich) and designated as formalin inactivated with Freund's adjuvant vaccine (FIF).
6
Immunization Strategies for Recombinant Proteins
7
Antibody Production Protocol with Adjuvants
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Freund’s complete and incomplete adjuvants, bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), HAT medium supplement and HT medium supplement were purchased from Sigma Aldrich (USA). Protein G resin and the peroxidase conjugated goat antimouse IgG (IgG-HRP) were purchased from TransGen Biotech (China). Other reagents were of chemical grade from Beijing Solarbio Science & Technology Co. Ltd. (China). Female Balb/c mice were purchased from the Animal Research Institute of Nanchang University (China). Animal manipulation was performed in compliance with institutional guidelines approved by the Nanchang University (Nanchang, China).
8
Optimized Immunoassay for Virus Detection
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Inactivated viruses 1 and 16 were kindly provided by the Yunnan Academy of Animal Husbandry and Veterinary Sciences. Goat anti-mouse IgG HRP conjugate was obtained from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). ZnCdSe/ZnS (core/shell) carboxylated orange-red QDs (emission wavelength [λem], 610 nm; particle size, 24 nm) were purchased from JiaYuan Quantum Dots Co., Ltd. (Wuhan, China). Freund’s complete and incomplete adjuvants, hydroxysuccinimide (NHS), 3,3,5,5-tetramethylbenzidine (TMB), carbodiimide hydrochloride (EDC), polyethylene glycol (PEG) 1500, fetal bovine serum (FBS), and 1640 medium were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Staphylococcal protein-A (SPA) was purchased from Aladdin (Shanghai, China). The rest of the chemicals and organic solvents were analytical grade or higher.
Opaque black plates were obtained from CoStar, Inc. (Cambridge, MA, USA). The gel imaging system (Gel Doc TM XR+) and electrophoresis instrument (MiniPROTEAN Tetra System) were both manufactured by Bio-Rad (CA, USA). SDS-PAGE electrophoresis and NanoDrop spectrophotometry were performed using a gel imaging system purchased from Bio-Rad (CA, USA). SpectraMaxi3 was purchased from Migu Molecular Instruments Co. Ltd. (Shanghai, China).
Opaque black plates were obtained from CoStar, Inc. (Cambridge, MA, USA). The gel imaging system (Gel Doc TM XR+) and electrophoresis instrument (MiniPROTEAN Tetra System) were both manufactured by Bio-Rad (CA, USA). SDS-PAGE electrophoresis and NanoDrop spectrophotometry were performed using a gel imaging system purchased from Bio-Rad (CA, USA). SpectraMaxi3 was purchased from Migu Molecular Instruments Co. Ltd. (Shanghai, China).
9
Colorimetric Biosensing Assay Development
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AA, hydrogen tetrachloroaurate (III) (HAuCl4), and trisodium citrate (Na3C6H5O7) were obtained from Wako Pure Chemical Industries (Japan). N-acryloxysuccinimide (NAS), bovine serum albumin (BSA), phosphate buffer saline (PBS), polyethylene glycol (PEG) 2000, 3,3ʹ, 5,5ʹ-
Tetramethylbenzidine (TMB), Tween 20, goat antirabbit IgG-HRP, Freund’s complete and incomplete adjuvants and protein A antibody purification kits, and other chemicals were supplied from Sigma Aldrich. Double distilled water (maximum conductivity of 18 MΩ), pap pens, and plastic backing sheet were supplied by Daido Sangyo, Japan.
Tetramethylbenzidine (TMB), Tween 20, goat antirabbit IgG-HRP, Freund’s complete and incomplete adjuvants and protein A antibody purification kits, and other chemicals were supplied from Sigma Aldrich. Double distilled water (maximum conductivity of 18 MΩ), pap pens, and plastic backing sheet were supplied by Daido Sangyo, Japan.
10
Immunoassay Protocol for HSA Detection
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Human Serum Albumin (HSA), Anti-rabbit IgG alkaline phosphatase conjugate, Protein-A agarose affinity column, p-nitrophenyl phosphate, Freund’s complete and incomplete adjuvants, were purchased from Sigma–Aldrich, USA. D-Glucose was from Sisco Research Lab (SRL), India. Flat bottom maxisorp enzyme linked immune sorbent assay (ELISA) modules were purchased from Nunc, Denmark. All other reagents/chemicals were also purchased highest analytical grade.
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