The GC×GC-TOFMS (Pegasus 4D, LECO Corporation, St. Joseph, MI) was equipped with a rail autosampler (MPS, Gerstel, Linthicum Heights, MD) and fitted with a two-dimensional column set consisting of an Rxi-624Sil MS (60 m × 250 μm × 1.4 μm (length × internal diameter × film thickness); Restek Corporation, Bellefonte, PA) first column followed by a Stabilwax (Crossbond Carbowax polyethylene glycol; 1 m × 250 μm × 0.5 μm; Restek) second column. The primary oven ramped at 5.0 °C/min from 35 °C to 230 °C, with a 5 min hold at 230 °C. The secondary oven, and quad-jet modulator (2.0 s modulation period, 0.5 s alternating hot and cold pulses), were heated in step with the primary oven with +5 °C and +25 °C offsets, respectively. The helium carrier gas flow rate was 2 mL/min. A splitless injection was used, with a 180 s desorption time. The inlet and transfer line temperatures were set to 270 °C and 250 °C, respectively. Time-of-flight mass spectra were acquired over the range of 30 to 500 m/z, with an acquisition rate of 200 spectra/s, and a 70 eV electron ionization. Data acquisition and analysis were performed using ChromaTOF software, version 4.50 (LECO Corp.).
Rxi 624sil ms
Manufactured by Restek
Sourced in Panama, Australia, United States
The Rxi-624Sil MS is a fused silica column designed for gas chromatography-mass spectrometry (GC-MS) applications. It features a 624-type stationary phase with a siloxane film for enhanced inertness and improved separation of a wide range of analytes.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using rxi 624sil ms
1
Comprehensive Metabolite Profiling with GC×GC-TOFMS
2
GC×GC-TOF-MS Analysis of Complex Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SPME fibres were desorbed directly into the GC×GC inlet at 250 • C for 5 min. The GC×GC analyses were performed with a LECO Pegasus 4D ® (LECO, Australia) consisting of an Agilent 7890A GC (Agilent Technologies, Australia) with a dual stage quad jet thermal modulator, secondary oven and Pegasus TOFMS. The column configurations consisted of a Rxi ® 624Sil MS (Restek Corporation, Australia) primary column (30.0 m × 0.25 mm i.d., with a 1.4 µm coating), connected to a Stabilwax ® secondary column (Restek Corporation, Australia) (2 m × 0.25 mm i.d., with a 0.5 µm coating). Helium was used as the carrier gas for all analyses and held at a flow rate of 1 mL/min. All injections were performed manually and were carried out in splitless mode. The GC oven temperature was initially set to 35 • C and held for 5 min, ramping to 240 • C at a rate of 5 • C/min and held for 5 min. The secondary oven temperature offset was 10 • C. Data were acquired at a rate of 100 spectra per second and processed using ChromaTOF ® (version 4.51.6.0; LECO).
3
Headspace GC Analysis of IPA in Formulations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IPA content in the formulations was determined by the HS-GC method. The headspace sampler was a pressure-loop HT3 model (Teledyne Tekmar, USA). The HS parameters used were: GC cycle time 19.25 min, valve oven temperature 105 o C, transfer line temperature 110 o C, platen temperature 80 o C, platen temperature equilibration time 0.5 min, sample equilibration time 25 min, vial pressure 204.7 kPa, pressurization time 1 min, pressure equilibration time 0.5 min, loop fill pressure 170.3 kPa, loop fill time 1 min and inject time 1 min.
The GC system used was a 7890 model (Agilent Technologies, USA), equipped with split/splitless injector (8:1 split ratio, 150 °C) and a flame ionization detector maintained at 300 o C, with gas flows of 450 mL min -1 air, 50 mL min -1 hydrogen and 25 mL min -1 helium makeup. The capillary column used was a 30 m × 0.32 mm × 1.8 µm Rxi ® -624Sil MS (Restek Corporation, USA). Column initial temperature was 50 o C maintained for 3.5 min, then ramped to 120 o C at 40 o C min -1 and kept for 6 min. Helium was used as a carrier gas flowing at a rate of 5.695 mL min -1 .
The GC system used was a 7890 model (Agilent Technologies, USA), equipped with split/splitless injector (8:1 split ratio, 150 °C) and a flame ionization detector maintained at 300 o C, with gas flows of 450 mL min -1 air, 50 mL min -1 hydrogen and 25 mL min -1 helium makeup. The capillary column used was a 30 m × 0.32 mm × 1.8 µm Rxi ® -624Sil MS (Restek Corporation, USA). Column initial temperature was 50 o C maintained for 3.5 min, then ramped to 120 o C at 40 o C min -1 and kept for 6 min. Helium was used as a carrier gas flowing at a rate of 5.695 mL min -1 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!