Twenty four molecules were used to evaluate their in vitro activity against Acanthamoeba castellanii Neff. Gallic acid, vanillin, caffeic acid, ferulic acid, chlorogenic acid, p-coumaric acid, m-coumaric acid, ellagic acid, vanillic acid, syringic acid, tyrosol, protochatechuic acid, rutin, catechin oleuropein and hydroxytyrosol were purchased from Sigma Aldrich (Tres Cantos, Madrid, Spain), the luteolin, lueolin-7- O -glucoside, apigenine, versbascoside, quercetin and the ursolic acid were purchased from Extrasynthese (Cymit quimica, Barcelona, Spain), as for the oleanolic and maslinic acids they were isolated and purified from olive leaf extraction accordingly to Sifaoui et al, (2014a) [5 (link)]. Stock solutions have been prepared by dissolving the molecules in the dimethyl sulfoxide (DMSO; Sigma Aldrich (Tres Cantos, Madrid, Spain) at a concentration of 10 mg/ml.
Apigenine
Manufactured by Extrasynthese
Sourced in Spain
Apigenine is a compound isolated from natural sources. It functions as a pure chemical standard for analytical and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Caffeic acid, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
Ellagic acid, by Merck Group (1 mentions)
Vanillic acid, by Merck Group (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Syringic acid, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
Vanillin, by Merck Group (1 mentions)
Tyrosol, by Merck Group (1 mentions)
M coumaric acid, by Merck Group (1 mentions)
Hydroxytyrosol, by Merck Group (1 mentions)
Quercetin, by Extrasynthese (1 mentions)
Luteolin, by Extrasynthese (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Ursolic acid, by Extrasynthese (1 mentions)
Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions)
Amentoflavone, by Extrasynthese (1 mentions)
Hinokiflavone, by Extrasynthese (1 mentions)
2 protocols using apigenine
1
Antiamoebic Activity of Phenolic Compounds
2
Poly(A) RNA Template Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Homopolymeric adenine (Poly (A)) RNA template was obtained from GE Healthcare. Hinokiflavone (ref 1017), Apigenine (ref 1102S) and Amentoflavone (ref 1057S) were purchased from Extrasynthese and from SigmaAldrich for Quercetin (ref Q4951). Compounds were resuspended in 100 % DMSO at 20 mM and stored at −20 °C. 3’dUTP (ref N-3005) was purchased from Trilink. Quant-it Picogreen® dsDNA assay kit (ref P11496) was obtained from Thermofisher scientific.
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