Anti cd3 antibody clone 2gv6

Manufactured by Roche

Sourced in United States, Azerbaijan

The Anti-CD3 antibody (clone 2GV6) is a laboratory reagent used for the detection and analysis of CD3-expressing cells. It is a monoclonal antibody that specifically binds to the CD3 complex, a key component of the T-cell receptor (TCR) complex. This antibody can be used in various immunoassays and cell-based applications to identify and characterize T-cell populations.

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Lab products found in correlation

Autostainer plus, by Agilent Technologies (2 mentions) Ventana benchmark stainer, by Roche (2 mentions) Benchmark ultra, by Roche (1 mentions) Anti cd68 antibody clone kp1, by Agilent Technologies (1 mentions) E cadherin clone nch38, by Agilent Technologies (1 mentions)

Close spelling variants of this product

CD3 (clone 2GV6, (11 citations) CD3 (2GV6, (11 citations) Anti-CD3 (8 citations) Anti-CD3 (2GV6) (6 citations) Anti-CD3 (clone 2GV6, (4 citations) Clone 2GV6 (4 citations)

3 protocols using anti cd3 antibody clone 2gv6

Immunohistochemical Analysis of Tumor-Infiltrating Lymphocytes

Cited 1 time

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Tissue microarrays (TMAs) were constructed as previously described using a semiautomated arraying device (TMArrayer, Pathology Devices, Westminister, MD, USA) [26 (link)]. Duplicate tissue cores (1 mm) were obtained from two different blocks of the primary tumor.
For immunohistochemical analysis of FoxP3 and CD8, 4 μm TMA-sections were automatically pre-treated using the PT Link system and then stained in an Autostainer Plus (Dako; Glostrup, Denmark) with the anti-FoxP3 antibody (clone 236A/E7, mouse, dilution 1:200, Abcam, Cambridge, UK), and the anti-CD8 antibody (clone C8/144B, mouse; dilution, 1:50; product M7103; Dako). For immunohistochemical analysis of CD3, 4 μm TMA-sections were pretreated using ULTRA Cell Conditioning Solution 1, pH 8.5 (Ventana Medical Systems Inc.Tucson, AZ, USA) for heat induced epitope retrieval, and stained in a Ventana BenchMark stainer (Ventana Medical Systems Inc.) with the anti-CD3 antibody (clone 2GV6, pre-diluted, Ventana Medical Systems Inc).
Analysis of CD20 and IGKC was performed as previously described [33 (link)].

Svensson M.C., Warfvinge C.F., Fristedt R., Hedner C., Borg D., Eberhard J., Micke P., Nodin B., Leandersson K, & Jirström K. (2017). The integrative clinical impact of tumor-infiltrating T lymphocytes and NK cells in relation to B lymphocyte and plasma cell density in esophageal and gastric adenocarcinoma. Oncotarget, 8(42), 72108-72126.

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Tissue Microarray Analysis of Immune Markers

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Formalin‐fixed, paraffin‐embedded tumor specimens from patients who met the inclusion criteria were used for tissue microarray construction. We marked one representative tumor area on the H&E‐stained slides. We arrayed a cylindrical 3‐mm tissue core from the corresponding paraffin block into a recipient block using an automated tissue processor (Tissue Microprocessor KIN‐2; Azumaya). In total, 545 patients had adequate cores available for immunohistochemical analysis.
Subsequently, we obtained 4‐μm sections from tissue microarray blocks and stained them with anti‐RNF128 (clone poly, 1:100; Abcam), anti‐CD3 antibody (clone 2GV6, prediluted; Ventana Medical Systems), anti‐CD4 antibody (clone SP35, prediluted; Ventana Medical Systems), anti‐CD45RO antibody (clone UCHL1, 1:200; Leica), anti‐CD25 antibody (clone 4C9, prediluted; Nichirei), anti‐CD68 antibody (clone KP1, 1:50; Dako), anti‐CD163 antibody (clone MRQ‐26, prediluted; Cell Marque), E‐cadherin (clone NCH‐38, prediluted; Dako), and vimentin (clone V9, 1:200; Dako) using a BenchMark ULTRA automated immunohistochemical slide staining system (Ventana Medical Systems) in accordance with the manufacturer's guidelines. Diaminobenzidine was used as the chromogen, whereas hematoxylin was used as the nuclear counterstain. The positive control tissues were stained alongside the study samples.

Fujimoto S., Kyuichi K., Kaede Y., Chihiro Y., Emi I., Ryo I., Reiji H., Toshiki Y, & Yokomise H. (2023). RNF128 expression in lung adenocarcinoma is a favorable prognostic factor associated with decreased tumor‐associated macrophages. Thoracic Cancer, 14(17), 1581-1588.

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Immunohistochemical Analysis of CD8, FoxP3, and CD3

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For immunohistochemical (IHC) analysis of CD8 and FoxP3, 4 μm TMA‐sections were pretreated using the PT Link system, and subsequently stained with the anti‐CD8 antibody (clone C8/144B, mouse; dilution, 1:50; product M7103; Dako) and the anti‐FoxP3 antibody (clone236A/E7, mouse, dilution 1:200, Abcam, Cambridge, UK) using the Autostainer Plus (Dako; Glostrup, Denmark).
For IHC analysis of CD3, 4 μm TMA‐sections were pretreated using ULTRA Cell Conditioning Solution 1, pH 8.5 (Ventana Medical Systems Inc., Tucson, AZ) for heat induced epitope retrieval, and stained in a Ventana BenchMark stainer (Ventana Medical Systems) with the anti‐CD3 antibody (clone 2GV6, prediluted, Ventana Medical Systems).

Berntsson J., Svensson M.C., Leandersson K., Nodin B., Micke P., Larsson A.H., Eberhard J, & Jirström K. (2017). The clinical impact of tumour‐infiltrating lymphocytes in colorectal cancer differs by anatomical subsite: A cohort study. International Journal of Cancer, 141(8), 1654-1666.

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