Mmpsense 750 fast

Manufactured by PerkinElmer

Sourced in United States

MMPSense 750 FAST is a fluorescent imaging agent designed for the in vivo detection of matrix metalloproteinase (MMP) activity. It is a non-invasive tool that can be used to visualize and quantify MMP activity in real-time.

Automatically generated - may contain errors

Lab products found in correlation

18 protocols using mmpsense 750 fast

Fluorescence Imaging of Protease Activity

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used vascular fluorescent mediated tomography (FMT) imaging with near-infrared fluorescent protease activatable probes as previously described [15] (link), [42] (link). Open chest FMT imaging of fibulin-4 mice was performed using an FMT 2500 system (Perkin Elmer Inc.) at 680- and 750-nm excitation and emission wavelengths, respectively, at five hours after tail vein injection of 4 nmol of Neutrophil Elastase 680 FAST and 2 nmol of MMPsense 750 FAST (Perkin Elmer Inc.). Mice with open chests were fixed into the portable animal imaging cassette that lightly compressed the mouse between optically translucent windows. The FMT 2500 quantitative tomography software was then used to calculate 3D fluorochrome concentration distribution of Neutrophil Elastase 680 FAST and MMPsense 750 FAST.
After open chest fluorescence imaging, complete lungs were harvested and fluorescence was quantified using the FMT 2500 or Odyssey imaging systems (LI-COR Inc.). Near-infrared images were obtained in the 680- and 700-nm channels, respectively.

Ramnath N.W., van de Luijtgaarden K.M., van der Pluijm I., van Nimwegen M., van Heijningen P.M., Swagemakers S.M., van Thiel B.S., Ridwan R.Y., van Vliet N., Vermeij M., Hawinkels L.J., de Munck A., Dzyubachyk O., Meijering E., van der Spek P., Rottier R., Yanagisawa H., Hendriks R.W., Kanaar R., Rouwet E.V., Kleinjan A, & Essers J. (2014). Extracellular Matrix Defects in Aneurysmal Fibulin-4 Mice Predispose to Lung Emphysema. PLoS ONE, 9(9), e106054.

+ Open protocol

+ Expand

In Vivo Bioluminescence and Fluorescence Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

For bioluminescence, mice received an intraperitoneal injection (150 mg/kg) of VivoGlo™ Luciferin (Promega) 5 min before imaging. For fluorescence, mice received an intravenous injection (2 nmol per mouse) of MMPSense™ 750 FAST (PerkinElmer) 18 h before imaging. Signals plus grey-scale photographic images were acquired using a Photon Imager™ (Biospace) and M3 Vision (Biospace). We maintained animals under general anaesthesia with 1–2% isoflurane, plus warming and monitoring, during image acquisition. We carried out signal quantification (photons/s/cm²/sr) using M3 Vision (Biospace).

Green D., Eyre H., Singh A., Taylor J.T., Chu J., Jeys L., Sumathi V., Coonar A., Rassl D., Babur M., Forster D., Alzabin S., Ponthan F., McMahon A., Bigger B., Reekie T., Kassiou M., Williams K., Dalmay T., Fraser W.D, & Finegan K.G. (2020). Targeting the MAPK7/MMP9 axis for metastasis in primary bone cancer. Oncogene, 39(33), 5553-5569.

+ Open protocol

+ Expand

Ex vivo Fluorescence Molecular Tomography

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ex vivofluorescence molecular tomography (FMT) was performed to evaluate macrophage accumulation and matrix metalloproteinase (MMP) activity as previously reported
^{25
,
37)}. We used near-infrared fluorescent nanoparticles: Aminospark 680 (Ex/Em=673/690 nm) and MMPSense 750 FAST (Ex/Em=749/775 nm) (PerkinElmer Inc., Waltham, MA, NEV10142, and NEV10168). These nanoparticles were administered via tail vein 24 h before imaging. After harvest of the heart and aorta, fluorescent signals from these nanoparticles were captured by dual-channel FMT (FMT2000, PerkinElmer, Inc.).

Katsuki S., Koga J.I., Matoba T., Umezu R., Nakashiro S., Nakano K., Tsutsui H, & Egashira K. (2021). Nanoparticle-Mediated Delivery of Pitavastatin to Monocytes/Macrophages Inhibits Angiotensin II-Induced Abdominal Aortic Aneurysm Formation in Apoe −/− Mice. Journal of Atherosclerosis and Thrombosis, 29(1), 111-125.

+ Open protocol

+ Expand

In Vivo Assessment of MMP Activity

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

MMP activity was assessed in vivo using MMPSense 750 FAST (PerkinElmer, Waltham, MA, United States), an activatable fluorescent imaging agent for MMP-2, -3, -7, -9, -12, and -13 according to the manufacturer’s instructions (2 nmol/subject i.v.). Measurements were performed with IVIS Lumina II (PerkinElmer, Waltham, MA, United States; auto acquisition time, Binning = 2, F/stop = 1, excitation/emission filter: 745/800 nm) 6 h later. ROIs were applied around the ankles and fluorescence was expressed as radiant efficiency ([photons/s/cm²/sr]/[μW/cm²]) (Borbély et al., 2015 (link)).

Horváth Á., Botz B., Kiss T., Csekő K., Kiss I., Felinger A., Szabados T., Kenyeres É., Bencsik P., Mócsai A., Ferdinandy P, & Helyes Z. (2019). Subantimicrobial Dose Doxycycline Worsens Chronic Arthritis-Induced Bone Microarchitectural Alterations in a Mouse Model: Role of Matrix Metalloproteinases?. Frontiers in Pharmacology, 10, 233.

+ Open protocol

+ Expand

In Vivo Tumor MMP Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

Twelve C57/B6 male mice (6 SF and 6 SC) were injected subcutaneously with 1×10⁵ live TC1 cells into the lateral aspect of the right thigh using the same protocol previously described, 28 days after tumor cell inoculation, mice were intravenously injected with 2 nmoles of the matrix metalloproteinase (MMP) probe MMPSense™ 750 FAST (PerkinElmer, Product number: NEV10168), this MMP activatable agent is optically silent upon injection and produces fluorescent signal after cleavage by disease related MMP's. Activation can occur by a broad range of MMP's including MMP 2, 3, 7, 9, 12, and 13. Two days before the MMPSense injection, all the fur lining the tumor area was removed by hair removal cream, after which mice were imaged 6, 12 and 24 hours post injection using the Xenogen IVIS Spectrum (Perkin Elmer) at the University of Chicago Optical Imaging Core Facility. The tumor near infrared fluorescence (average radiant efficiency) of images was quantified using Living Image Software (Perkin Elmer).

Hakim F., Wang Y., Zhang S.X., Zheng J., Yolcu E.S., Carreras A., Khlayfa A., Shirwan H., Almendros I, & Gozal D. (2014). Fragmented sleep accelerates tumor growth and progression through recruitment of tumor-associated macrophages and TLR4 signaling. Cancer research, 74(5), 1329-1337.

+ Open protocol

+ Expand

Fluorescent In Vivo Imaging of MMPs in Chagas Disease

Check if the same lab product or an alternative is used in the 5 most similar protocols

We were the first researchers to use a fluorescent probe to detect matrix metalloproteinase (MMP) by IVIS technique in Chagas disease. The MMPSense 750 FAST (PerkinElmer, Inc., Boston, MA) is a MMP activatable agent that is optically silent upon injection but produces fluorescent signal (761 nm excitation and 789 nm emission) after cleavage by MMP-2, -3, -7, -9, -12 and -13. Control or chagasic animals treated with PBS or MSC received by tail vein a dose of 2 nM MMPSense in 100 μL of PBS one month after therapy (the sample number was 4-6 in each group, the same number of organs exposed in Figure 5). After 48 hours the images from ventral surface and ex vivo tissues (heart, bladder, lung, liver, spleen, kidney, leg muscle, brown and white fat) were acquired using the IVIS Kodak Image Station configured as described in item 2.6.

J.a.s.m.i.n., Jelicks L.A., Tanowitz H.B., Peters V.M., Mendez-Otero R., de Carvalho A.C, & Spray D.C. (2014). Molecular imaging, biodistribution and efficacy of mesenchymal bone marrow cell therapy in a mouse model of Chagas disease. Microbes and infection / Institut Pasteur, 16(11), 923-935.

+ Open protocol

+ Expand

Fluorescent Imaging of Arthritis Progression

Check if the same lab product or an alternative is used in the 5 most similar protocols

An intravenous injection of fluorescent imaging agent (MMP Sense 750 FAST, PerkinElmer, Waltham, MA, USA) specific for MMP3 was given at a recommended dose of 2 nmol/100 μl per mouse (as per the manufacturer’s instruction) to measure the progression of arthritis [27 (link)]. MMP Sense FAST is an activatable fluorescent imaging compound that is optically silent upon injection but produces fluorescent signal after cleavage by disease-related MMPs. The signals emitted were detected using the IVIS system (Caliper Life Sciences, MA, USA) which is an optimized set of high-efficiency filters and spectral un-mixing algorithms to measure the light emission across the blue to near infrared wavelength region.

Chukkapalli S., Rivera-Kweh M., Gehlot P., Velsko I., Bhattacharyya I., Calise S.J., Satoh M., Chan E.K., Holoshitz J, & Kesavalu L. (2016). Periodontal bacterial colonization in synovial tissues exacerbates collagen-induced arthritis in B10.RIII mice. Arthritis Research & Therapy, 18, 161.

+ Open protocol

+ Expand

Dual-isotope and fluorescent imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols

For radioactive imaging, a mixture of ¹H-217 and ³H-217 was injected intravenously (150 µCi/ml PBS for total 0.7 mM; 10 mg/kg into 12-wk-old mice). Mice were anesthetized, and body temperature was maintained at 38°C for 1 h before sacrifice. Mice were immersed in dry ice/isopentane to prevent tissue redistribution, and whole-body sections (20 µm) were prepared at −20°C with a slicing microtome (Leica Microsystems). Sagittal sections were desiccated (24 h, RT) before radioimaging (β-imager TM 2000, Biospace Lab). For fluorescence imaging, 4-wk-old mice were injected with MMPSense750 Fast (intravenously, 0.08 nmol/g; PerkinElmer) and sacrificed 7 h later. Organs were extracted and imaged (Maestro system; Cambridge Research and Instrumentation). Multispectral image cubes containing multiple 10-nm bandwidth fluorescence emission signals were acquired at identical exposure times and spectrally unmixed using the fluorescence emission spectra from the injected compound.

Saw S., Aiken A., Fang H., McKee T.D., Bregant S., Sanchez O., Chen Y., Weiss A., Dickson B.C., Czarny B., Sinha A., Fosang A., Dive V., Waterhouse P.D., Kislinger T, & Khokha R. (2019). Metalloprotease inhibitor TIMP proteins control FGF-2 bioavailability and regulate skeletal growth. The Journal of Cell Biology, 218(9), 3134-3152.

+ Open protocol

+ Expand

Measuring MMP Activity in VSMCs

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

For in vitro MMP activity, VSMCs expressing wild type or TGFR2^G357W and ACTA2^R179H were co-transfected with 30 nM of siCTRL or siHDAC9 for 48hrs as described above. After 24hrs of growing medium 30 µg of total protein were prepared from siCTRL and siHDAC9 treated cells to measure the MMP activity by gelatin zymography^{59 (link)}. 3D plot was generated using ImageJ software. For in vivo MMP activity assay, mice were tail vein injected with 600uL of MMPSense 750 FAST, a near-infrared fluorescence sensor for MMP2 and MMP9 activity, (PerkinElmer, USA). Mice were killed 24 h post injection and aortas were dissected and analyzed using a Kodak image station 4000MM Pro for macroscopic fluorescence reflectance molecular imaging^{64 (link)}.

Lino Cardenas C.L., Kessinger C.W., Cheng Y., MacDonald C., MacGillivray T., Ghoshhajra B., Huleihel L., Nuri S., Yeri A.S., Jaffer F.A., Kaminski N., Ellinor P., Weintraub N.L., Malhotra R., Isselbacher E.M, & Lindsay M.E. (2018). An HDAC9-MALAT1-BRG1 complex mediates smooth muscle dysfunction in thoracic aortic aneurysm. Nature Communications, 9, 1009.

+ Open protocol

+ Expand

Measuring Intra-articular MMP Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 20 Lewis rats were used for detection of MMP activity. Intra-articular levels of MMP activity were assessed as an indication of inflammatory and catabolic processes. At day 1 post-injury, while under isoflurane anesthesia, both knees of each animal received an intra-articular injection 50 μL of MMPSense 750FAST (PerkinElmer, Boston, MA) resuspended in PBS as recommended by the manufacturer. Animals were then placed in an IVIS Spectrum Imaging System (PerkinElmer) under isoflurane anesthesia, and the fluorescent signal of MMPSense750 monitored using excitation/emission wavelengths of 745/800nm. Circular regions of interest (ROI) encasing the knee joints were created, as described in the biodistribution study.

Lewis S., Baird P., Evans R.G., Ghali W.A., Wright C.J., Gibson E, & Baylis F. (2001). Dancing with the porcupine: rules for governing the university–industry relationship. CMAJ: Canadian Medical Association Journal, 165(6), 783-785.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!