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Agilent low input linear amplification kit

Manufactured by Agilent Technologies
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The Agilent Low Input Linear Amplification kit is a laboratory tool designed to amplify low-input DNA or RNA samples. It provides linear amplification to generate high-quality, representative copies of the original sample material.

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Lab products found in correlation

Agilent whole mouse genome arrays g4122f, by Agilent Technologies (3 mentions) Genepix 4000b scanner, by Molecular Devices (2 mentions) Genepix pro 5, by Molecular Devices (2 mentions) Nanodrop nd 1000 spectrophotometer, by Agilent Technologies (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Rneasy mini column, by Qiagen (1 mentions)

3 protocols using agilent low input linear amplification kit

1

Microarray Analysis of Mouse Transcriptome

Cited 1 time
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This was performed as in our previous publications (Pendergrass et al., 2012 (link)). cDNA samples were amplified and labeled using the Agilent Low Input Linear Amplification kit (Agilent Technologies, Santa Clara, CA) and were hybridized against Universal Mouse Reference (UMR) (Strategene, La Jolla, CA) to Agilent Whole Mouse Genome arrays (G4122F) (Agilent Technologies, Santa Clara, CA) in a common reference based design. Microarrays were hybridized and washed in accordance with manufacturer’s protocols and scanned using a dual laser GenePix 4000B scanner (Axon Instruments, Foster City, CA). The pixel intensities of the acquired images were then quantified using GenePix Pro 5.1 software (Axon Instruments, Foster City, CA).
Long K.B., Li Z., Burgwin C.M., Choe S.G., Martyanov V., Sassi-Gaha S., Earl J., Eutsey R., Ahmed A., Ehrlich G.D., Artlett C.M., Whitfield M.L, & Blankenhorn E.P. (2014). The Tsk2/+ mouse fibrotic phenotype is due to a gain-of-function mutation in the PIIINP segment of the Col3a1 gene. The Journal of investigative dermatology, 135(3), 718-727.
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2

Microarray Analysis of Mouse Transcriptome

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
This was performed as in our previous publications (Pendergrass et al., 2012 (link)). cDNA samples were amplified and labeled using the Agilent Low Input Linear Amplification kit (Agilent Technologies, Santa Clara, CA) and were hybridized against Universal Mouse Reference (UMR) (Strategene, La Jolla, CA) to Agilent Whole Mouse Genome arrays (G4122F) (Agilent Technologies, Santa Clara, CA) in a common reference based design. Microarrays were hybridized and washed in accordance with manufacturer’s protocols and scanned using a dual laser GenePix 4000B scanner (Axon Instruments, Foster City, CA). The pixel intensities of the acquired images were then quantified using GenePix Pro 5.1 software (Axon Instruments, Foster City, CA).
Long K.B., Li Z., Burgwin C.M., Choe S.G., Martyanov V., Sassi-Gaha S., Earl J., Eutsey R., Ahmed A., Ehrlich G.D., Artlett C.M., Whitfield M.L, & Blankenhorn E.P. (2014). The Tsk2/+ mouse fibrotic phenotype is due to a gain-of-function mutation in the PIIINP segment of the Col3a1 gene. The Journal of investigative dermatology, 135(3), 718-727.
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3

Whole Mouse Genome Microarray Analysis

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from mouse skin samples using standard Trizol (Invitrogen, Carlsbad, CA) procedures. Samples were manually homogenized as previously described (2 (link)). Samples were further purified using RNA cleanup with RNeasy mini-columns (Qiagen, Valencia, CA). RNA was quantified on a NanoDrop ND-1000 Spectrophotometer (Agilent Technologies, Santa Clara, CA) and integrity assessed by gel electrophoresis.
Samples were amplified and labeled using the Agilent Low Input Linear Amplification kit (Agilent Technologies, Santa Clara, CA) and were hybridized against Universal Mouse Reference (UMR) (Strategene, La Jolla, CA) to Agilent Whole Mouse Genome arrays (G4122F) (Agilent Technologies, Santa Clara, CA) in a common reference based design as previously described (2 (link), 5 (link)).
Sargent J.L., Li Z., Aliprantis A.O., Greenblatt M., Lemaire R., Wu M.H., Wei J., Taroni J., Harris A., Long K.B., Burgwin C., Artlett C.M., Blankenhorn E.P., Lafyatis R., Varga J., Clark S.H, & Whitfield M.L. (2016). Interspecies Comparative Genomics Identifies Optimal Mouse Models of Systemic Sclerosis. Arthritis & rheumatology (Hoboken, N.J.), 68(8), 2003-2015.
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