The animal protocols used for the study were approved by the Committee on the Use and Care of Animals (Chongqing University Cancer Hospital, Chongqing, China), and the research was performed in accordance with the institution's guidelines. Mice were housed at an ambient temperature of 22 ℃, with a humidity of 30–70%, and a light cycle of 12 h on/12 h off set from 8 am to 8 pm. A375-shNC and A375-shSNF5 tumor xenografts were established by subcutaneously inoculating 5 × 106 cells into flanks of six-week-old female BALB/c nude mice. Before injecting the tumor cells, eight-week-old female C57BL/6 J mice were shaved at the flank; then, 5 × 106 B16-shNC and B16-shSNF5 tumor cells were injected into the shaved flank subcutaneously.
We depleted CD8+ T cells by using 100 ug/mL of intraperitoneally injected anti- CD8α antibody (2.43, Bio X Cell) on days −3, 0, 3, and 6. Tumor size was measured using a caliper and calculated using the following formula: volume = (length)(width)2/2.
We depleted CD8+ T cells by using 100 ug/mL of intraperitoneally injected anti- CD8α antibody (2.43, Bio X Cell) on days −3, 0, 3, and 6. Tumor size was measured using a caliper and calculated using the following formula: volume = (length)(width)2/2.