Assessment of NK cell function by flow cytometry was carried out as previously described(30 ). Briefly, PBMCs (n=6) and targets cells were co-cultured and stained with FITC conjugated anti-CD107a (93937, BioLegend). An hour later, Golgi Stop and Golgi Plug (BD Biosciences) was added and cells were incubated for 3 hours. Cells were stained with Live/Dead Fixable Aqua Staining Kit (Thermo Fisher), PE-CY7 conjugated anti-CD56 and PE-CF594 conjugated anti-CD3, PE conjugated anti-CD69 (310906 (FN50), BioLegend), fixed and permeabilized. Permeabilized cells were stained with BV650 conjugated IFNγ (93705 (4S.B3), BioLegend) and BV421 conjugated TNFα (562783 (Mab11), BD Biosciences).
Bv421 conjugated tnfα 562783 mab11
Manufactured by BD
BV421 conjugated TNFα (562783 (Mab11)) is a labelled antibody product. It is a fluorochrome-conjugated monoclonal antibody that targets tumor necrosis factor alpha (TNFα), a cytokine involved in various immune responses. The BV421 fluorochrome allows for flow cytometric detection and analysis of TNFα-expressing cells.
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Lab products found in correlation
2 protocols using bv421 conjugated tnfα 562783 mab11
1
Assessing Natural Killer Cell Function
2
Assessing Natural Killer Cell Function
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