Cisapride monohydrate and quinine were obtained from Sigma Aldrich (Saint Louis, MO). Verapamil hydrochloride was obtained from MP Biomedicals (Solon, OH). Quinidine was obtained from Tocris (Bristol, UK). Frozen stocks of human iPSC-CMs from a healthy individual (SCVI-480CM) were obtained from Joseph C. Wu, MD, PhD at the Stanford Cardiovascular Institute Biobank. The iPSC-CM line was derived from an African American female donor, and was approved by Stanford University Human Subjects Research Institutional Review Board. The cells were differentiated to cardiomyocytes as described previously (Churko et al., 2013 (link); Burridge et al., 2014 (link)). HEK-HCN1 (CT6114) cells were obtained from Charles River (Wilmington, MA).
Cisapride monohydrate
Manufactured by Merck Group
Sourced in United States
Cisapride monohydrate is a chemical compound used in laboratory settings. It functions as a prokinetic agent, which means it helps to stimulate the movement of the gastrointestinal tract. This compound is often utilized in research and development activities related to the study of digestive system function and disorders.
Automatically generated - may contain errors
Lab products found in correlation
Formaldehyde solution, by Merck Group (3 mentions)
B 27 medium supplement, by Thermo Fisher Scientific (3 mentions)
Earlytox cardiotoxicity kit, by Molecular Devices (3 mentions)
Mitotracker orange cmtmros reagent, by Thermo Fisher Scientific (3 mentions)
Recombinant oncostatin m, by Thermo Fisher Scientific (3 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (3 mentions)
Hank s balanced salt solution, by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Hoechst 33342, by Thermo Fisher Scientific (3 mentions)
Verapamil hydrochloride, by MP Biomedicals (2 mentions)
Hek hcn1 ct6114 cells, by Charles River Laboratories (2 mentions)
Quinine, by Merck Group (2 mentions)
Quinidine, by Bio-Techne (2 mentions)
Gentamicin 50 mg ml, by Thermo Fisher Scientific (2 mentions)
Tetraoctylammonium bromide, by Merck Group (2 mentions)
Hydrogen peroxide 3, by Thermo Fisher Scientific (2 mentions)
Urethane, by Merck Group (1 mentions)
Amiodarone hydrochloride, by Merck Group (1 mentions)
Crizotinib, by Merck Group (1 mentions)
Forskolin, by Merck Group (1 mentions)
7 protocols using cisapride monohydrate
1
Cardiotoxicity Screening using iPSC-CMs
2
Rat Study of Urethane and Cisapride
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Urethane was purchased from Sigma-Aldrich (Saint Louis, MO, USA). Cisapride monohydrate (Sigma-Aldrich) was dissolved in vehicle (10% dimethyl sulfoxide; Sigma-Aldrich). Urethane (20%, 0.8 g/kg) and cisapride (0.2, 0.02, and 0.002 mg/kg) [11 (link)–13 (link)] were used in separate cohorts of rats.
3
Evaluation of Cardiac and Hepatic in vitro Models
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iCell Cardiomyocytes (Catalog No. CMC-100-010-001; Lot No. 1031999) and Hepatocytes (Catalog No. PHC-100-020-001; Lot No. 1636 and 1208), including plating and maintenance media, were purchased from Cellular Dynamics International (Madison, WI). EarlyTox Cardiotoxicity Kits, including reference standards isoproterenol, propranolol, and sotalol, and CatchPoint cAMP GPCR assay kits were purchased from Molecular Devices, LLC (Sunnyvale, CA). B-27 medium supplement, CellROX Deep Red reagent, gentamicin (50 mg/mL), Hank's Balanced Salt Solution, HCS LipidTOX Deep Red reagent, Hoechst 33342, MitoTracker Orange CMTMRos reagent, penicillin/streptomycin solution, and RPMI 1640 medium were obtained from Life Technologies (Grand Island, NY). 3-Isobutyl-1-methylxanthine, amiodarone hydrochloride, cisapride monohydrate, crizotinib, doxorubicin hydrochloride, formaldehyde solution, forskolin, Krebs-Ringer bicarbonate buffer (KRBG), sodium bicarbonate, and tetraoctylammonium bromide (TAB) were all obtained from Sigma-Aldrich (St. Louis, MO). Dimethyl sulfoxide (DMSO), dexamethazone, hydrogen peroxide (3%), menadione, recombinant oncostatin M, and sunitinib were purchased from Fisher Scientific (Waltham, MA).
4
Comprehensive Cardiac and Hepatic Assay Protocol
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iCell cardiomyocytes (Catalogue #: CMC-100-010-001) and hepatocytes (Catalogue #: PHC-100-020-001), including their respective plating and maintenance media were obtained from Cellular Dynamics International (Madison, WI). EarlyTox Cardiotoxicity kits were purchased from Molecular Devices LLC (Sunnyvale, CA). Reference standard compounds (isoproterenol, sotalol, and propranolol) were included in these kits. Hank's Balanced Salt Solution, RPMI 1640 medium, B-27 medium supplement, gentamicin (50 mg ml–1), penicillin/streptomycin solution, Hoechst 33342, and MitoTracker Orange CMTMRos reagent were all purchased from Life Technologies (Grand Island, NY). Cisapride monohydrate, tetraoctyl ammonium bromide, and formaldehyde solution were purchased from Sigma-Aldrich (St. Louis, MO). Dimethyl sulfoxide (DMSO), dexamethazone, hydrogen peroxide (3%), and recombinant oncostatin M were obtained from Fisher Scientific (Waltham, MA).
5
Pharmacological Evaluation of Human iPSC-Cardiomyocytes
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Cisapride monohydrate and quinine were obtained from Sigma Aldrich (Saint Louis, MO). Verapamil hydrochloride was obtained from MP Biomedicals (Solon, OH). Quinidine was obtained from Tocris (Bristol, UK). Frozen stocks of human iPSC‐CMs from a healthy individual (SCVI‐480CM) were obtained from Joseph C. Wu, MD, PhD, at the Stanford Cardiovascular Institute Biobank. The iPSC‐CM line was derived from an African‐American female donor and was approved by Stanford University Human Subjects Research Institutional Review Board. The cells were differentiated to cardiomyocytes as described previously (Burridge et al., 2014 (link); Churko et al., 2013 (link)). HEK‐HCN1 (CT6114) cells were obtained from Charles River (Wilmington, MA).
6
Evaluating hERG Blockers Potency
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In this study, five hERG channel blockers [astemizole (Sigma-Aldrich, St. Louis, MO, USA), cisapride monohydrate (Sigma-Aldrich), E-4031 hydrochloride (Wako pure chemical, Tokyo, Japan), quinidine (Sigma-Aldrich), and terfenadine (Sigma-Aldrich)] were used. Three concentrations of each drug were applied to the same cell cumulatively and sequentially. Three hundred eighty-four wells could be served with solution at the same time due to the availability of the 384 pipetting head of the system.
7
Cardiac and Hepatic Cell Culture Protocols
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