Chromatographic grade acetonitrile (≥99.9%) and methanol (≥99.9%) were obtained from Fisher (Pittsburgh, PA, USA). Analytical grade toluene (≥99.5%) and dichloromethane (≥99.5%) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai China). Analytical grade ferrous chloride tetrahydrate (FeCl2·4H2O) (99.0%), ferric trichloride hexahydrate (FeCl3·6H2O) (98.0%), tetramethylsilane (TEOS) (98.0%), ammonium hydroxide (25.0–28.0%), azobisisbutyronitrile (AIBN) (98.0%), 3-methacryloxypropyltrimethoxysilane (MPTS) (97.0%), 4-vinyl benzoic acid (4-VBA) (97.0%), 4-vinyl pyridine (4-VP) (95.0%), divinylbenzene (DVB) (80%), and trimethylpropane trimethacrylate (TRIM) (98.0%) were obtained from Energy Chemical (Shanghai, China). Methacrylic acid (MAA) (99%) and ethylene glycol dimethacrylate (EGDMA) (98%) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Distilled water was purchased from Guangzhou Watson's Food & Beverage Co., Ltd. (Guangzhou, China). The standard references including CBZ (99.0%), LTG (≥98.0%), oxcarbazepine (OXC) (≥98.0%), phenobarbital (PB) (≥98.0%), phenytoin (PHT) (≥98.0%), primidone (PRM) (≥98.0%), and benzoguanamine (BGA) (≥98.0%) were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China) and their chemical structures were shown in Fig. S1.†
Chromatographic grade acetonitrile
Manufactured by Thermo Fisher Scientific
Sourced in United States, China
Chromatographic grade acetonitrile is a high-purity solvent used in analytical chemistry and chromatographic techniques. It is a colorless, volatile liquid with a characteristic odor. The high purity and low impurity levels of this product make it suitable for use in sensitive analytical applications, such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and other separation techniques.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (10 mentions)
Formic acid, by Thermo Fisher Scientific (4 mentions)
Millipore water purification system, by Merck Group (2 mentions)
Ammonium hydroxide, by Energy Chemical (1 mentions)
Dichloromethane, by Sinopharm (1 mentions)
Deltamethrin, by Merck Group (1 mentions)
Bifenthrin, by Merck Group (1 mentions)
Cyhalothrin, by Merck Group (1 mentions)
Fenpropathrin, by Merck Group (1 mentions)
Technical grade permethrin, by Merck Group (1 mentions)
Menthol, by Thermo Fisher Scientific (1 mentions)
Milli q plus purification system, by Merck Group (1 mentions)
Oxypaeoniflorin, by Yuanye Bio-Technology (1 mentions)
Albiflorin, by Yuanye Bio-Technology (1 mentions)
Benzoylpaeoniflorin, by Yuanye Bio-Technology (1 mentions)
1 2 3 4 6 o pentagalloylglucose, by Yuanye Bio-Technology (1 mentions)
Interleukin 1 beta il 1 β, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Yuanye Bio-Technology (1 mentions)
Orientin, by Yuanye Bio-Technology (1 mentions)
Isoorientin, by Yuanye Bio-Technology (1 mentions)
16 protocols using chromatographic grade acetonitrile
1
Synthesis and Characterization of Magnetic Polymer Microspheres
2
Pesticide Biodegradation Assay Protocol
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Technical-grade permethrin, deltamethrin, bifenthrin, fenpropathrin, cyhalothrin, and beta-cypermethrin were obtained from Sigma-Aldrich, USA. Chromatographic grade acetonitrile was purchased from Fisher Scientific, USA. All other reagents used here were obtained from Sigma-Aldrich, USA. The chemicals were dissolved in acetone or dimethyl sulfoxide (DMSO) at a stock concentration of 10 g·L−1, and stored in dark bottles at 4°C prior to use.
The mineral salt medium (MSM) containing 2 g (NH4)2SO4, 0.2 g MgSO4·7H2O, 0.01 g CaCl2·2H2O, 0.001 g FeSO4·7H2O, 1.5 g Na2HPO4·12H2O, and 1.5 g KH2PO4 per liter was used for biodegradation assays in the study. The Luria-Bertani medium (LB) containing 10 g tryptone, 5 g yeast extract and 10 g NaCl per liter was used for incubating Acinetobacter baumannii ZH-14. Both of the media were autoclaved at 121°C for 20 min. The pH of the media was adjusted to 7.0.
The mineral salt medium (MSM) containing 2 g (NH4)2SO4, 0.2 g MgSO4·7H2O, 0.01 g CaCl2·2H2O, 0.001 g FeSO4·7H2O, 1.5 g Na2HPO4·12H2O, and 1.5 g KH2PO4 per liter was used for biodegradation assays in the study. The Luria-Bertani medium (LB) containing 10 g tryptone, 5 g yeast extract and 10 g NaCl per liter was used for incubating Acinetobacter baumannii ZH-14. Both of the media were autoclaved at 121°C for 20 min. The pH of the media was adjusted to 7.0.
3
Paeoniae Radix Alba Analytical Protocol
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Paeoniae Radix Alba was supplied by Lanzhou Foci Pharmaceutical Co., Ltd. (Qinghai, China) and authenticated by Professor Jingjuan Wang (Beijing University of Chinese Medicine, Beijing, China). Chromatographic-grade acetonitrile, menthol and formic acid was supplied by Fisher Scientific. Reference standards (purity ≥ 90%) of paeoniflorin, gallic acid, benzoic acid were purchased from National Institutes for Food and Drug Control (Beijing, China). Reference standards (purity ≥ 90%) of benzoylpaeoniflorin, oxypaeoniflorin, albiflorin, and 1,2,3,4,6-O-pentagalloylglucose were purchased from Shanghai Yuanye Biological Technology Co., Ltd. (Shanghai, China). Ultrapure water was purified by the Millipore Milli Q plus purification system. All other reagents used were of analytical grade and commercially available.
4
HPLC-MS/MS Analysis of Nimodipine
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Chromatographic grade acetonitrile, methanol, and formic acid were obtained from Fisher Scientific (Fair Lawn, NJ, United States). Ultrapure water was obtained from a Milli-Q purification system (Billerica, MA, United States). FA (PurityAbove 90%, Lot No. 1007456) and nimodipine tablets (Batch No. BJ29160) were purchased from Changbaishan Pharmaceutical Co. Ltd. (Jilin, China) and Bayer Healthcare Company Ltd. (Leverkusen, Germany), respectively. Physiologic saline solution and anesthetic were supplied from Chengdu Mansite Bio-technology Co., Ltd. (Sichuan, China). ELISA kits of nitric oxide (NO), superoxide dismutase (SOD), malondialdehyde (MDA), and nitric oxide synthase (NOS) were purchased from Chengdu Mansite Bio-technology Co., Ltd. (Sichuan, China). ELISA kits of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were bought from Sigma-Aldrich (St Louis, MO, United States). ELISA kits of vascular endothelial growth factor (VEGF), hypoxia inducible factor-1α (HIF-1α), and matrix metallopeptidase 2 (MMP-2) were provided by Carlo Erba (Val de Reuil, France). Standard substance leucine enkephalin (Purity99.4%, Lot No. 1912032) was obtained from Lonza (Barcelona, Estado Anzoátegui, Spain).
5
Comprehensive Analysis of Litsea gracile Herb
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Chromatographic‐grade acetonitrile and methanol were obtained from Fisher Scientific. Formic acid in MS grade was provided by Sigma‐Aldrich. Distilled water was produced via a Millipore water purification system (Millipore). All other chemicals were of analytical grade. For standards, luteolin (111,520–201,605), and chlorogenic acid (110,753–202,018) were purchased from NIFDC (National Institutes for Food and Drug Control, Changchun, Jilin, China). Isoorientin (B21528), orientin (B20143), vitexin (B20875), isovitexin (B21544), cynaroside (B20887), DPPH, ABTS, and 2,4,6‐Tris(2‐pyridyl)‐s‐triazine (TPTZ) were obtained from Shanghai Yuanye Bio‐Technology Co., Ltd.
Fifteen batches of L. gracile herbal materials were collected from their main origin in China and identified by Prof. Jiyu Gong of Changchun University of Chinese Medicine, School of Pharmaceutical Sciences. The information of samples is shown in TableS1 .
Fifteen batches of L. gracile herbal materials were collected from their main origin in China and identified by Prof. Jiyu Gong of Changchun University of Chinese Medicine, School of Pharmaceutical Sciences. The information of samples is shown in Table
6
Phytochemical Profiling of Schisandra chinensis
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Chromatographic grade acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid in MS grade was purchased from Sigma-Aldrich (St. Louis, MO, USA). Distilled water was prepared in-house via a Millipore water purification system (Millipore, Billerica, MA, USA). All other chemicals were analytical grade. For standards, schisandrol A (110857-201815), schisandrin A (110764-201915), and schisandrin B (110765-201813) were purchased from NIFDC (National Institute of Food and Drug Control, Changchun, Jilin, China).
Schisandrol B (wkq18030609), schizantherin B (wkq1802351), schisanhenol (wkq1803209), and schisandrin C (wkq18030718) were obtained from Victory Biological Technology (Sichuan, China). Gomisin D (B20437), angeloylgomisin H (B20143), schisantherin A (B21348), and gomisin G (B20438) were obtained from Shanghai Yuanye Biotechnology Co. Ltd. (Shanghai, China) .
The materials of Schisandra chinensis fructus were collected at Jilin in 2019 and identified by Prof. Jiyu Gong (School of Pharmaceutical Sciences, Changchun University of Chinese Medicine). The detailed sample information was listed in Table S2.
Schisandrol B (wkq18030609), schizantherin B (wkq1802351), schisanhenol (wkq1803209), and schisandrin C (wkq18030718) were obtained from Victory Biological Technology (Sichuan, China). Gomisin D (B20437), angeloylgomisin H (B20143), schisantherin A (B21348), and gomisin G (B20438) were obtained from Shanghai Yuanye Biotechnology Co. Ltd. (Shanghai, China) .
The materials of Schisandra chinensis fructus were collected at Jilin in 2019 and identified by Prof. Jiyu Gong (School of Pharmaceutical Sciences, Changchun University of Chinese Medicine). The detailed sample information was listed in Table S2.
7
Chromatographic Analysis of Organic Solvents
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Chromatographic grade acetonitrile (batch number 19085176) and methanol (batch number 19085029) were produced by Thermofisher Company (Waltham, MA, USA), chromatography grade acetic acid (batch number 20180813) was produced by Tianjin Damao Chemical Reagent Factory (Tianjin, China), and ultrapure water was made by the laboratory. The products are shown in Table 6 , all purchased from Chengdu Pusi-Biotechnology Co., Ltd., and the purity is ≥98%.
8
Racemic Penthiopyrad Extraction Protocol
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Racemic penthiopyrad (purity, 99.5%), manufactured by Dr. Ehrenstorfer GmbH (Augsburg, Germany), was purchased from J&K Scientific Ltd. (Beijing, China). Chromatographic grade acetonitrile (ACN, 99.9%), methanol (MeOH, 99.9%), and formic acid (FA, 8.0%) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Analytical grade sodium chloride (NaCl, 99.5%), anhydrous sodium sulfate (Na2SO4, 99.0%), ethanol (EA, 99.7%), linear alkylbenzene sulfonates (LAS, 99.0%), and acetic acid (AA, 99.5%) were provided by Tianjin Zhiyuan Reagent Co., Ltd. (Tianjin, China). Primary secondary amine (PSA, 99.5%) was bought from Agela Technologies (Tianjin, China), and a 0.22 μm nylon syringe filter was obtained from PeakSharp Technologies (Beijing, China).
9
Multiplex Proteomic Analysis of Thyroid Hormones
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iTRAQ® Reagents Multiplex Kit and Ammonium formate were purchased from Sigma (CA, USA). Levothyroxine sodium tablets (L-T4) were obtained from MerckSerono (Darmstadt, Germany). Saline solution was purchased from Medlian Pharmaceutical Co., Ltd. (Harbin, China). Tissue Protein Extraction Kit and BCA Protein Assay Kit were obtained from CWbiotech Co., Ltd. (Shanghai, China). Triiodothyronine (T3) Assay Kit and Thyroxine (T4) Assay Kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The estrogen (E2), testosterone (T), cAMP, and cGMP ELISA kits were obtained from USCN Life Science Inc. (Wuhan, China). Trypsin was purchased from Promega Corporation (Wisconsin, USA). Vivacon 500 tubes were purchased from Satorius (Gottingen, Germany). TRIzol reagent was obtained from Life technologies (New York, USA). The reverse transcription kit and fluorescent dye were obtained from Roche (Switzerland). All primers were purchased from ComateBio Company (Jilin, China). Chromatographic grade acetonitrile, methanol, and ultrapure water were obtained from Thermo Fisher (MA, USA).
10
Extraction and Characterization of Aucubin from Elm Samaras
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Samaras of E. ulmoides were obtained from Leshan (Sichuan, China) and authenticated by Prof. Yuangang Zu from the Northeast Forestry University in China. Dried samaras of E. ulmoides were pulverized using a grinder and were sieved (250–380 μm), then stored in closed vacuum desiccators at 4 °C in the dark prior to use.
Aucubin standard (purity ≥98%) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Chromatographic grade acetonitrile was purchased from Thermo Fisher Scientific (Shanghai, China). 1-Butyl-3-methylimidazolium bromide (BmimBr) ionic liquid was purchased from Shanghai Chengjie Chemical Co. Ltd. (Shanghai, China) and used without further purification. The water used in all experiments was freshly prepared with a Milli-Q water cleansing apparatus (Millipore, MA, USA).
Aucubin standard (purity ≥98%) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Chromatographic grade acetonitrile was purchased from Thermo Fisher Scientific (Shanghai, China). 1-Butyl-3-methylimidazolium bromide (BmimBr) ionic liquid was purchased from Shanghai Chengjie Chemical Co. Ltd. (Shanghai, China) and used without further purification. The water used in all experiments was freshly prepared with a Milli-Q water cleansing apparatus (Millipore, MA, USA).
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