Tgfbr1

Manufactured by Proteintech

Sourced in United Kingdom

TGFBR1 is a protein-coding gene that encodes the transforming growth factor-beta receptor type I. This receptor is involved in the signaling pathway of the transforming growth factor-beta (TGF-β) family of cytokines, which play a role in regulating cellular processes such as cell growth, differentiation, and apoptosis.

Automatically generated - may contain errors

Lab products found in correlation

Smad2, by Proteintech (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Thiazolyl blue tetrazolium bromide mtt, by Merck Group (1 mentions) Ha tag, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Puromycin, by Merck Group (1 mentions) Mg132 s2619, by Selleck Chemicals (1 mentions) P smad3, by Abcam (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (1 mentions) P smad2, by Proteintech (1 mentions) Recombinant human tgf β1 protein 240 b, by R&D Systems (1 mentions) Smad3, by Proteintech (1 mentions) Tgfbr1, by Thermo Fisher Scientific (1 mentions) Tgfbr1, by R&D Systems (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Ecl detection system, by Bio-Rad (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions)

2 protocols using tgfbr1

Investigating TGF-β1 Signaling Pathway

Check if the same lab product or an alternative is used in the 5 most similar protocols

Recombinant Human TGF-β1 Protein (240-B) was from R&D systems. Lipofectamine® 2000 or Lipofectamine® 3000 Transfection Reagent was from Invitrogen. Bafilomycin A1, Cycloheximide (CHX), puromycin, Thiazolyl Blue Tetrazolium Bromide (MTT, M5655) were from Sigma. MG132 (S2619) was from Selleck. TGFBR1 inhibitor LY-364947 (616451) was from Calbiochem. The primary antibodies used for western blotting were as follow: POH1 (CST, 4197, 1:1000), p-SMAD3 (abcam, ab52903, 1:1000), p-SMAD2 (CST, 3108, 1:1000), SMAD3 (proteintech, 25494-1-AP, 1:1000), SMAD2 (proteintech, 12570-1-AP, 1:1000), GAPDH (Santa Cruz, sc-25778, 1:1000), Flag-tag (Sigma Aldrich, f1804, 1:1000), TGFBR1 (Thermo Fisher, PA5-14959, 1:500), TGFBR1 (Proteintech, 55391-1-AP, 1:500), TGFBR1 (Goat anti-TGFBR1, R&D, AF3025-SP), TGFBR2 (Themo Fisher, PA5-35076, 1:500), β-actin (Santa Cruz, sc-47778, 1:1000), HA-tag (Sigma Aldrich, H9658, 1:1000), V5-tag (Mouse anti-V5, MBL, M167-3, 1:1000), V5-tag (Rabbit anti-V5, CST, 13202S, 1:1000), CAV1 (proteintech, 16447-1-AP, 1:500) and ubiquitin (abcam, EPR8589, 1:1000). The primary antibodies used for immunohistochemistry were as follow: POH1 (Sigma, HPA002114), TGFBR1 (Thermo Fisher, PA5-14959, 1:100) and TGFBR2 (Themo Fisher, PA5-35076, 1:100).

Wang B., Xu X., Yang Z., Zhang L., Liu Y., Ma A., Xu G., Tang M., Jing T., Wu L, & Liu Y. (2019). POH1 contributes to hyperactivation of TGF-β signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-β receptors and caveolin-1. EBioMedicine, 41, 320-332.

+ Open protocol

+ Expand

Protein Expression Analysis of TNBC Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The total protein of TNBC cells was exacted with RIPA buffer and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, then electransferred onto a PVDF membrane (Bio-Rad, CA, USA). The membranes were blocked with 5% skim milk and incubated with antibodies against Cyclin D1 (1:1000, Proteintech, USA), p27 (1:1000, Proteintech), Bax (1:1000, Proteintech), Bcl-2 (1:1000, Proteintech), E-cadherin (1:2000, Proteintech), Vimentin (1:1000, CST, Danvers, USA), N-cadherin(1:1000, Proteintech), p-ERK1/2 (1:1000, Abcam, Cambridge, UK); ERK1/2 (1:2000, Abcam), Smad2(1:500, Proteintech), Smad7(1:500, Proteintech),TGFBR1(1:500, Proteintech) and GADPH (1:5000, CST) at 4°C overnight and then incubated with secondary antibodies (1:5000, Proteintech) at room temperature for 2 h. Finally, the bands were examined by Immobilob™ Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA) and were detected by Immunoblots visualized by ECL detection system (Quantity One software, BioRad).

Ni K., Huang Z., Zhu Y., Xue D., Jin Q., Zhang C, & Gu C. (2021). The lncRNA ADAMTS9-AS2 Regulates RPL22 to Modulate TNBC Progression via Controlling the TGF-β Signaling Pathway. Frontiers in Oncology, 11, 654472.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!