Sb277011a dihydrochloride

DA hydrochloride (Lot# BCBG8676V), pramipexole dihydrochloride (Batch# 0000069160), (±)-7-Hydroxy-2-(di-n-propylamino) tetralin hydrobromide (7-OH-DPAT, Lot# 037M4153V), desipramine hydrochloride (Lot# 107K0730) and nomifensine maleate (Lot# 087K4064) were purchased from Sigma-Aldrich (St. Louis, MO). SB277011A dihydrochloride (Batch# 3B/219051) was purchased from Tocris Bioscience (Bristol, UK). Purified (18.2 MΩ) water was used to prepare all aqueous solutions. The artificial cerebrospinal fluid (aCSF) modified for zebrafish contained 131 mM NaCl, 2 mM KCl, 1.25 mM KH₂PO₄, 20 mM NaHCO₃, 2 mM MgSO₄, 10 mM glucose, 2.5 mM CaCl₂·H₂O, and 10 mM HEPES (pH 7.4). Perchloric acid (0.2 M) was used to prepare the DA stock solutions and aCSF without glucose was used to dilute the stock solutions for electrode calibrations. Stock solutions of 50 μM 7-OH-DPAT, 50 μM pramipexole, 10 μM nomifensine, and 10 μM SB277011A, were freshly prepared before each experiment. 7-OH-DPAT and SB277011A stock solutions were prepared in DMSO and nomifensine and pramipexole stock solutions were prepared in aCSF.