Log In Sign up
The largest database of trusted experimental protocols

Eclipse 700

Manufactured by Nikon
Visit Supplier
Sourced in United States

The Nikon Eclipse 700 is a high-performance microscope designed for laboratory use. It features a sturdy construction and advanced optical systems to provide clear and detailed images. The Eclipse 700 is capable of various microscopy techniques, including brightfield, darkfield, and phase contrast, making it a versatile tool for a wide range of applications.

Automatically generated - may contain errors

Lab products found in correlation

Goat or donkey serum, by Jackson ImmunoResearch (3 mentions) Volocity image analysis software, by PerkinElmer (3 mentions) Foxp3, by Thermo Fisher Scientific (1 mentions) Cd11c, by BD (1 mentions) Cytoseal 60 medium, by Thermo Fisher Scientific (1 mentions) Streptavidin hrp, by Agilent Technologies (1 mentions) Harris hematoxylin solution, by Merck Group (1 mentions) Biotinylated goat anti rabbit secondary antibody, by Vector Laboratories (1 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions) Image pro plus software, by Media Cybernetics (1 mentions)

6 protocols using eclipse 700

1

Scratch Assay for Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
The scratch assay allows for the preliminary examination of the effects of TMZ, Gefitinib, BCNU, and UA on the migration of U-251 MG cells. Cells were treated with each compound, at a sub-toxic concentration, to prevent cytotoxic responses but potentially inhibit migration. U-251 MG cells were seeded at 0.9 × 106 cells in individual 35 mm dishes and incubated for 24 h. A scratch was performed in each dish prior to treatment using a 200 µL sterile pipette tip. Utilising the data observed from the UA dose response curve, a sub-toxic concentration (12 μM) was chosen. Cells were treated with either media alone, DMSO (0.1%), or 12.5 μM UA, each scratch was examined under a light microscope and images were taken using the (Nikon Eclipse 700). Multiple images were taken for each time point and the average size of scratch for that time point was obtained. Image analysis was performed using image processing and analysis software, Image J [62 (link)].
Conway G.E., Zizyte D., Mondala J.R., He Z., Lynam L., Lecourt M., Barcia C., Howe O, & Curtin J.F. (2021). Ursolic Acid Inhibits Collective Cell Migration and Promotes JNK-Dependent Lysosomal Associated Cell Death in Glioblastoma Multiforme Cells. Pharmaceuticals, 14(2), 91.
+ Open protocol
+ Expand
2

Scratch Assay for Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols
The scratch assay allows for the preliminary examination of the effects of TMZ, Gefitinib, BCNU, and UA on the migration of U373MG cells. Cells were treated with each compound, at a subtoxic concentration; to prevent cytotoxic responses but potentially inhibit migration. U373MG cells were seeded at 0.9 x 10 6 cells in individual 35mm dishes and incubated for 24hrs. A scratch was performed in each dish prior to treatment using a 200µl sterile pipette tip. Utilising the data observed from the UA dose response curve a sub-toxic concentration (12μM) was chosen. Cells were treated with either media alone, DMSO (0.1%), or 12.5μM UA, each scratch was examined under a light microscope and images were taken using the (Nikon Eclipse 700). Multiple images were taken for each time point and the average size of scratch for that time point was obtained. Image analysis was performed using image processing and analysis software, Image J (Schneider et al., 2012) (link).
Conway G.E., Zizyte D., Mondala J.R.M., He Z., Lynam L., Lecourt M., Barcia C., Howe O., & Curtin J.F. (2020). Ursolic acid inhibits cell migration and promotes JNK-dependent lysosomal associated cell death in Glioblastoma multiforme cells.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of Lymphoid Tissues

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used for immunohistochemistry diluted 1:200: ERTR7 (scbt, clone sc-73355), CD11c (BD, clone HL3), Laminin alpha 4 (Novus Bio, clone 775830), laminin alpha 5 (Novus Bio, polyclonal), Foxp3 (ThermoFisher, clone NRRF-30), Y-Ae (scbt, clone sc-32247). For immunohistochemistry, LNs and spleens were frozen with OCT compound (Scigen Tissue-Plus). Frozen sections were cut at 6μm, fixed with cold acetone, blocked with 5% goat or donkey serum (Jackson ImmunoResearch, West Grove, PA) and incubated with the indicated antibodies and DAPI. Samples were incubated with secondary antibodies diluted 1:400 for 1 h (Rabbit IgG, Goat polyclonal, Jackson Immunoresearch; Rat IgG Goat Polyclonal, Jackson Immunoresearch; Rabbit IgG, Donkey Polyclonal, Jackson Immunoresearch; Rat IgM, Goal Polyclonal Jackson Immunoresearch). Samples were further processed as described previously45 (link),46 (link),56 (link). Images were acquired with a Nikon Eclipse 700 (Nikon, Melville, NY, USA) and analyzed with Volocity image analysis software (version 4.7.2) Perkin Elmer, Waltham, MA). The positive staining area percentage was quantified based on at least three independent experiments with 3 sections per LN and 3–5 fields/section. Number of mice are indicated in figure legends.
Gammon J.M., Carey S.T., Saxena V., Eppler H.B., Tsai S.J., Paluskievicz C., Xiong Y., Li L., Ackun-Farmmer M., Tostanoski L.H., Gosselin E.A., Yanes A.A., Zeng X., Oakes R.S., Bromberg J.S, & Jewell C.M. (2023). Engineering the lymph node environment promotes antigen-specific efficacy in type 1 diabetes and islet transplantation. Nature Communications, 14, 681.
+ Open protocol
+ Expand
4

Immunohistochemical Analysis of CREBBP

Check if the same lab product or an alternative is used in the 5 most similar protocols
Airway sections were formalin fixed and paraffin embedded prior to immunohistochemical staining. Sections were deparaffinized, rehydrated, processed for antigen retrieval and incubated overnight at 4 °C with CREBBP antibody (Table 2). Sections were subsequently incubated with a biotinylated goat anti-rabbit secondary antibody (1:100, Vector Laboratories, Burlingame, CA, USA) prior to visualization with Streptavidin-HRP (Dako) and 3,3-diaminobenzidine (Dako). Slides were counterstained with Harris Hematoxylin solution (Sigma, St. Louis, MO, USA) and dehydrated before coverslipping with Cytoseal 60 medium (Richard-Allan Scientific, Kalamazoo, MI, USA).
Using the Nikon Eclipse 700 (Nikon Instruments, Melville, NY, USA) with a 60× objective and SPOT Advanced software (Diagnostic Instruments, Sterling Heights, MI, USA), five images were obtained from each section. These images were analyzed for positively and negatively stained nuclei using ImagePro Plus software (Media Cybernetics, Rockville, MD, USA).
Stefanowicz D., Ullah J., Lee K., Shaheen F., Olumese E., Fishbane N., Koo H.K., Hallstrand T.S., Knight D.A, & Hackett T.L. (2017). Epigenetic modifying enzyme expression in asthmatic airway epithelial cells and fibroblasts. BMC Pulmonary Medicine, 17, 24.
+ Open protocol
+ Expand
5

Immunohistochemistry and Whole Mount Analysis of Lymph Nodes and Pinna

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
LNs and spleens were fixed in 10% buffered formalin and embedded in paraffin. Sections were cut at 5μm and stained with H&E. For immunohistochemistry, organs were frozen with OCT compound (Scigen Tissue-Plus, Gardena, CA). Frozen sections were cut at 6μm, fixed with cold acetone, blocked with 5% goat or donkey serum (Jackson ImmunoResearch, West Grove, PA) and incubated with the indicated antibodies (Key resources table). For pinna whole mounts, ears were depilated, peeled into 2 pieces, and fixed with 4% paraformaldehyde (PFA) for 15 minutes at room temperature. Samples were further processed as described previously (Brinkman et al., 2016 (link); Piao et al., 2018 (link)). LEC monolayers were stained as described previously (Xiong et al., 2017 (link)). Images were acquired with a Nikon Eclipse 700 (Nikon, Melville, NY, USA) and analyzed with Volocity image analysis software (Perkin Elmer, Waltham, MA). The positive staining area percentage was quantified based on at least three independent experiments with 3 mice/group, 3 LN/mouse, 3 sections/LN, 3–5 fields/section.
Saxena V., Piao W., Li L., Paluskievicz C., Xiong Y., Simon T., Lakhan R., Brinkman C.C., Walden S., Hippen K.L., WillsonShirkey M., Lee Y.S., Wagner C., Blazar B.R, & Bromberg J.S. (2022). Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses. Cell reports, 39(3), 110727.
+ Open protocol
+ Expand
6

Immunohistochemistry and Whole Mount Analysis of Lymph Nodes and Pinna

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
LNs and spleens were fixed in 10% buffered formalin and embedded in paraffin. Sections were cut at 5μm and stained with H&E. For immunohistochemistry, organs were frozen with OCT compound (Scigen Tissue-Plus, Gardena, CA). Frozen sections were cut at 6μm, fixed with cold acetone, blocked with 5% goat or donkey serum (Jackson ImmunoResearch, West Grove, PA) and incubated with the indicated antibodies (Key resources table). For pinna whole mounts, ears were depilated, peeled into 2 pieces, and fixed with 4% paraformaldehyde (PFA) for 15 minutes at room temperature. Samples were further processed as described previously (Brinkman et al., 2016 (link); Piao et al., 2018 (link)). LEC monolayers were stained as described previously (Xiong et al., 2017 (link)). Images were acquired with a Nikon Eclipse 700 (Nikon, Melville, NY, USA) and analyzed with Volocity image analysis software (Perkin Elmer, Waltham, MA). The positive staining area percentage was quantified based on at least three independent experiments with 3 mice/group, 3 LN/mouse, 3 sections/LN, 3–5 fields/section.
Saxena V., Piao W., Li L., Paluskievicz C., Xiong Y., Simon T., Lakhan R., Brinkman C.C., Walden S., Hippen K.L., WillsonShirkey M., Lee Y.S., Wagner C., Blazar B.R, & Bromberg J.S. (2022). Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses. Cell reports, 39(3), 110727.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!