The human ovarian cancer cell line, TOV-21G (p53-WT), was purchased from American Type Culture Collection (ATCC). The human ovarian cancer cell lines A2780 (p53-WT), COV318 (p53-I195F), and COV362 (p53-Y220C) were purchased from the European Collection of Authenticated Cell Cultures (ECACC). TOV-21G and A2780 were cultured in Roswell Park Memorial Institute medium (RPMI) (Welgene, Daegu, Korea). COV318 and COV361 were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich; St. Louis, MO, USA). Both media contained 10% fetal bovine serum (HyClone; GE Healthcare), 100 U/mL penicillin, and 100 µg/mL streptomycin (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA). Cell lines were maintained in 5% CO2 and 37 °C humidified incubators. Cells were subcultured every 3–4 days.
A2780
Manufactured by Welgene
Sourced in Cameroon, United States
The A2780 is a laboratory equipment product used for cell culture applications. It is designed to provide a controlled environment for the growth and maintenance of cells. The core function of the A2780 is to regulate temperature, humidity, and gas composition to support the optimal growth and proliferation of cells.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Ovcar3, by Welgene (2 mentions)
Rpmi 1640 medium, by Welgene (2 mentions)
Ovcar3, by American Type Culture Collection (2 mentions)
Cov318, by Merck Group (1 mentions)
Tov 21g, by Welgene (1 mentions)
Hyclone, by GE Healthcare (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Hek293, by Welgene (1 mentions)
Skov3, by Welgene (1 mentions)
A2780 cell line, by European Collection of Cell Cultures (1 mentions)
Dulbecco s modified eagle s medium, by Welgene (1 mentions)
Hek293, by American Type Culture Collection (1 mentions)
Tov112d, by American Type Culture Collection (1 mentions)
Streptomycin, by Welgene (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
A2780, by American Type Culture Collection (1 mentions)
Penicillin, by Welgene (1 mentions)
Skov3, by Thermo Fisher Scientific (1 mentions)
4 protocols using a2780
1
Comparative Analysis of Ovarian Cancer Cell Lines
2
Characterization of Ovarian Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We were purchased the SKOV3 human epithelial ovarian cancer cell line from the Korean Cell Line Bank (KCLB, Seoul, Korea), and the OVCA429 and OVCA433 cell line were provided by the Korea Gynecologic Cancer Bank through the Bio and Medical Technology Development Program of the Ministry of Science, Information and Communication Technology, and Future Planning (MSIP, Seoul, Korea). The HEK293, TOV112D and OVCAR3 cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), and the A2780 cell line was procured from the European Collection of Cell Cultures (ECACC, London, UK). SKOV3, TOV112D and A2780 cells were cultured in RPMI-1640 medium (Welgene, Seoul, Korea), and HEK293, OVCAR3, OVCA429 and OVCA433 cells were cultured in Dulbecco’s Modified Eagle’s Medium (Welgene, Seoul, Korea). All culture media were supplemented with 10% foetal bovine serum and 1% penicillin/streptomycin, and cell lines were maintained at 37 °C in a humidified atmosphere of 5% CO2 and 95% air. Culture medium was replaced with fresh medium every 2–3 days, and cells were used between Passages 5 and 10. OVCA429 and OVCA433 cells were used between passages 10 and 20. The subtypes of the epithelial ovarian cancer cell lines are as follows: SKOV3, serous; TOV112D, endometrioid; A2780, non-specified; OVCAR3, carcinoma; OVCA429, serous; and OVCA433, serous [29 (link)].
3
Validation of Ovarian Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A2780 and OVCAR-3 human ovarian cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). A2780 and OVCAR-3 cells were cultured in DMEM (Welgene, Daegu, South Korea) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 mg/mL streptomycin (all from Welgene) and incubated at 37 °C under a humidified 95%/5% (v/v) mixture of air and CO2. To validate the phenotypic characteristics of the ovarian cancer cell lines A2780 and OVCAR-3, we performed Western blotting analyses and identified EpCAM expression (Supplementary Figure S3 ).
4
Ovarian Cancer Xenograft Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture. The human ovarian cancer cell line SK-OV-3 was purchased from the American Type Culture Collection (ATCC; no. HTB-77; Manassas, VA, USA) and A2780 was purchased from the European Collection of Cell Cultures (cat no. 93112519; Wiltshire, UK). SK-OV-3 cells were cultured in McCoy's 5A medium containing 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 µg/ml streptomycin (all from Gibco-BRL, Rockville, MD, USA) in a 95% humidified air and 5% CO 2 atmosphere at 37̊C. A2780 cells were cultured in RPMI-1640 medium (Welgene, Inc., Gyeongsangbuk-do, Korea) containing 10% FBS, 100 U/ml penicillin, and 100 µg/ml streptomycin (all from Gibco-BRL) in a 95% humidified air and 5% CO 2 atmosphere at 37̊C.
Ovarian cancer mouse xenograft model. All procedures for handling and euthanizing the animals used in this study were performed in strict compliance with the guidelines of the Korean Animal Protection Law and were approved by the Institutional Animal Care and Use Committee (IACUC) of Ewha Womans University School of Medicine. SK-OV-3 cells (2x10 6 ) suspended in culture media were intraperitoneally injected into 10 female nude mice (CAnN.Cg-Foxn1 NU , 4-6 weeks old). Four weeks after inoculation, the xenograft mice were sacrificed, and at least four implants adhering to the mesothelial surface of each mouse were harvested.
Ovarian cancer mouse xenograft model. All procedures for handling and euthanizing the animals used in this study were performed in strict compliance with the guidelines of the Korean Animal Protection Law and were approved by the Institutional Animal Care and Use Committee (IACUC) of Ewha Womans University School of Medicine. SK-OV-3 cells (2x10 6 ) suspended in culture media were intraperitoneally injected into 10 female nude mice (CAnN.Cg-Foxn1 NU , 4-6 weeks old). Four weeks after inoculation, the xenograft mice were sacrificed, and at least four implants adhering to the mesothelial surface of each mouse were harvested.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!