Beadblaster 24 microtube homogenizer

Manufactured by Benchmark Scientific

Sourced in United States

The BeadBlaster 24 Microtube Homogenizer is a laboratory device designed to efficiently homogenize and disrupt samples in microtubes. It utilizes high-speed oscillation to agitate samples containing beads, enabling effective cell lysis and sample preparation for downstream analysis.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (3 mentions) Iscript reverse transcription kit, by Bio-Rad (3 mentions) Itaq sybr green supermix, by Bio-Rad (3 mentions) Rlt lysis buffer, by Qiagen (3 mentions) Cfx96 touch real time pcr detection system, by Bio-Rad (3 mentions) Bca protein assay, by Thermo Fisher Scientific (1 mentions) Smart seq v4 ultra low input rna kit, by Takara Bio (1 mentions) Picopure rna isolation kit, by Arcturus (1 mentions) Nextseq 500 instrument, by Illumina (1 mentions) Nextera xt protocol, by Illumina (1 mentions) High sensitivity rna screen tape system, by Agilent Technologies (1 mentions) Rnalater, by Thermo Fisher Scientific (1 mentions) Spectramax gemini em, by Molecular Devices (1 mentions) Edta free protease inhibitor cocktail, by Merck Group (1 mentions) Ipvh00010, by Merck Group (1 mentions) Fgfr1, by Cell Signaling Technology (1 mentions) Zirconium beads, by Benchmark Scientific (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

BeadBlaster 24 (31 citations) BeadBlaster (11 citations) BeadBlaster homogenizer (9 citations) Beadblaster 24 homogenizer (7 citations) Microtube homogenizer (2 citations)

9 protocols using beadblaster 24 microtube homogenizer

Murine Cytokine and Chemokine Analysis

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Tumors excised from surrounding colon tissue were homogenized in 500 μl of 1% NP-40 lysis buffer (150 mM NaCl, 20 mM Tris HCl, pH 7.5) supplemented with protease and phosphatase inhibitors using 0.5 ml of 800 µm glass homogenization beads in screw-top blast microtubes placed in the Benchmark Bead Blaster 24 Microtube Homogenizer at 6 m/s for 30 s with 2-min breaks between the three cycles. Protein concentration in supernatants was quantified using BCA Protein Assay (Life Technologies). All samples were diluted to a uniform protein concentration of 4 mg/ml and submitted to Eve Technologies Corp. for Murine 31-plex cytokine and chemokine discovery arrays.

Feng Y., Yuan Q., Newsome R.C., Robinson T., Bowman R.L., Zuniga A.N., Hall K.N., Bernsten C.M., Shabashvili D.E., Krajcik K.I., Gunaratne C., Zaroogian Z.J., Venugopal K., Casellas Roman H.L., Levine R.L., Chatila W.K., Yaeger R., Riva A., Jobin C., Kopinke D., Avram D, & Guryanova O.A. (2023). Hematopoietic-specific heterozygous loss of Dnmt3a exacerbates colitis-associated colon cancer. The Journal of Experimental Medicine, 220(11), e20230011.

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Synovial Tissue RNA-Seq Profiling

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RNA was isolated from whole synovial tissue by homogenizing tissue with 3.0 mm high impact zirconium beads and a Bead Blaster 24 microtube homogenizer (Benchmark Scientific). RNA was extracted from the cell homogenate using a QIAGEN Plus Mini kit. RNA from sorted macrophages was extracted using a PicoPure RNA isolation kit according to manufacturer’s instructions (Arcturus Bioscience, Inc.). RNA quality and quantity were measured using a High Sensitivity RNA ScreenTape System (Agilent Technologies). Whole synovial tissue RNA-seq libraries were prepared from 90ng of total RNA using the NEBNext Ultra Kit with polyA-enrichment (NEB). RNA-seq libraries from sorted macrophage populations were prepared using a SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc.) followed by Nextera XT protocol (Illumina). RNA-seq libraries were sequenced on an NextSeq 500 instrument (Illumina Inc.) with ~5-10×10⁶ aligned reads per sample. A commercially available universal human RNA reference (uhRNA) was prepared along with the synovial RNA to represent background RNA expression.

Mandelin AM I.I., Homan P.J., Shaffer A.M., Cuda C.M., Dominguez S.T., Bacalao E., Carns M., Hinchcliff M., Lee J., Aren K., Thakrar A., Montgomery A.B., Bridges SL J.r., Bathon J.M., Atkinson J.P., Fox D.A., Matteson E.L., Buckley C.D., Pitzalis C., Parks D., Hughes L.B., Geraldino-Pardilla L., Ike R., Phillips K., Wright K., Filer A., Kelly S., Ruderman E.M., Morgan V., Abdala-Valencia H., Misharin A.V., Budinger G.S., Bartom E.T., Pope R.M., Perlman H, & Winter D.R. (2018). Transcriptional Profiling of Synovial Macrophages using Minimally Invasive Ultrasound-Guided Synovial Biopsies in Rheumatoid Arthritis. Arthritis & rheumatology (Hoboken, N.J.), 70(6), 841-854.

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Splenic Gene Expression Quantification

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Splenic tissues were collected in RNALater (Ambion) and incubated at 4°C overnight for inactivation. Tissues were homogenized using metal beads in a BeadBlaster 24 Microtube Homogenizer (Benchmark Scientific) with RLT lysis buffer (Qiagen). Total RNA was extracted by using RNeasy Mini Kit (Qiagen), and the cDNA was synthesized utilizing iScript Reverse Transcription kit (Bio-Rad). cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 0.5 μM each of gene-specific forward and reverse primer. qRT-PCR assays were performed on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad), and PCR assays were denatured for 30 s at 95°C, followed by 40 cycles of 15 s at 95°C, and 60 s at 60°C. To check specificity of amplification, melt curve analysis was performed. Relative quantitation of mRNA expression was calculated utilizing the 2^-ΔΔCT method. Primers used in qRT-PCR analysis are listed in S1 Table.

Gonzales C., Liang Y., Fisher J., Card G., Sun J, & Soong L. (2023). Alterations in germinal center formation and B cell activation during severe Orientia tsutsugamushi infection in mice. PLOS Neglected Tropical Diseases, 17(5), e0011090.

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Quantitative mRNA Expression Analysis

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Tissues were homogenized using metal beads in a BeadBlaster 24 Microtube Homogenizer (Benchmark Scientific) with RLT lysis buffer (Qiagen). RNA was extracted using RNeasy Mini kits (Qiagen) and the synthesis of cDNA was proceeded using an iScript Reverse Transcription kit (Bio-Rad). cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 5 μM each of gene-specific forward and reverse primers. The PCR assays were denatured for 30s at 95°C, followed by 40 cycles of 15s at 95°C, and 60s at 60°C, by utilizing the CFX96 Touch real-time PCR detection system (Bio- Rad). Relative quantitation of mRNA expression was calculated using the 2^−ΔΔCt method. The primers are listed in Table S1.

Liang Y., Fisher J., Gonzales C., Trent B., Card G., Sun J., Tumanov A.V, & Soong L. (2022). Distinct Role of TNFR1 and TNFR2 in Protective Immunity Against Orientia tsutsugamushi Infection in Mice. Frontiers in Immunology, 13, 867924.

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Cathepsin S Activity Assay in Mouse Lacrimal Glands

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Lysates were prepared by placing LG from male NOD and BALB/c mice at the ages indicated into 2.0 ml tubes with 2.0 mm zirconium beads. 300 μL of CTSS lysis buffer was added to each tube, and LG were homogenized using a BeadBlaster™ 24 microtube homogenizer (Benchmark Scientific, Inc. Edison, NJ). The homogenate was then centrifuged at 6000 X g for 10 min at 4°C; the supernatant collected was analyzed immediately. 10 μL of LG lysate with 40 μL of CTSS lysis buffer and 50 μL of CTSS reaction buffer or 100 μL of mouse tears diluted in CTSS reaction buffer were added to 96-well plates in duplicates. 2 μL of CTSS inhibitor was added to one of the two wells and 2 μL of substrate was added to each well with and without the CTSS inhibitor for each sample. The reaction was then incubated at 37°C for 1 h. The quantity of the resulting fluorescent products obtained from the CTSS and substrate reaction was measured with a microplate spectrofluorometer (Spectramax Gemini EM; Molecular Devices, Sunnyvale CA) using 400 nm/505 nm excitation/emission filters.

Janga S.R., Shah M., Ju Y., Meng Z., Edman M, & Hamm-Alvarez S.F. (2018). Longitudinal Analysis of Tear Cathepsin S Activity Levels in Male Non-Obese Diabetic Mice Suggests its Potential as an Early Stage Biomarker of Sjögren’s Syndrome. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals, 24(1), 91-102.

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Protein Extraction and Western Blot

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Tumors were snap-frozen and later homogenized in tissue lysis buffer (62.5 mM Tris-HCl pH 6.8, 2% SDS) containing cOmplete, EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich, 11873580001) with zirconium beads (Benchmark Scientific, D1132-30,) in BeadBlaster 24 Microtube Homogenizer. The homogenate was heated at 98°C for 8 minutes, and the protein concentrations were measured with the BCA Protein Assay Kit (ThermoFisher, 23227). The protein extracts were loaded on an SDS-PAGE system and then transferred to polyvinylidene difluoride membranes (Millipore, IPVH00010). The primary antibodies used include FGFR1 (Cell Signaling Technology, 9740, 1:1000) and β-Actin (Cell Signaling Technology, 3700, 1:5000).

Yuan X., Hao X., Chan H.L., Zhao N., Pedroza D.A., Liu F., Le K., Smith A.J., Calderon S.J., Lieu N., Soth M.J., Jones P., Zhang X.H, & Rosen J.M. (2024). CBP/P300 BRD Inhibition Reduces Neutrophil Accumulation and Activates Antitumor Immunity in TNBC. bioRxiv.

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Lipid Extraction and Liver Enzyme Quantification

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Liver samples were subjected to lipid extraction as previously described [56 (link),57 (link)]. Briefly, the samples were homogenized in a solution containing chloroform and methanol using the BeadBlaster 24 microtube homogenizer (Benchmark Scientific, Inc., Sayreville, NJ, USA) and gently shook overnight at 4 °C. After the incubation, the samples were added with 0.6% NaCl solution and centrifuged (4000× g for 20 min at 4 °C) for removal of the organic layer. The organic fraction was dried at room temperature, reconstituted in 100 µL of isopropanol and used for triglycerides’ (TGs’) quantification with an enzymatic/colorimetric kit (LABORLAB, Sao Paulo, SP, Brazil). Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured with the kinetic UV method using the ALT (GPT) or AST (GOT) Liquid Stable Reagent according to the manufacturer’s specifications (LABORLAB, Sao Paulo, SP, Brazil).

Assalin H.B., De Almeida K.C., Guadagnini D., Santos A., Teixeira C.J., Bordin S., Rocha G.Z, & Saad M.J. (2022). Proton Pump Inhibitor Pantoprazole Modulates Intestinal Microbiota and Induces TLR4 Signaling and Fibrosis in Mouse Liver. International Journal of Molecular Sciences, 23(22), 13766.

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Lung Viral Titering in Infected Mice

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Eight to 10-week old C57BL/6 mice were infected in the same manner as described above. Four days post-infection, mice were collected and given a lethal dose of ketamine-xylazine (400 uL). Once sufficiently anesthetized, a cervical dislocation was performed before removing the whole lung and trachea from the mice. Lung samples were then homogenized in 1 mL of PBS using the Benchmark BeadBlaster 24 Microtube Homogenizer. Homogenized samples were then spun to pellet the insoluble pieces of lung and the supernatant was collected, aliquoted and frozen at -80C. Lung supernatants were then thawed and titered using the plaque assay protocol listed above.

Harding A.T., Haas G.D., Chambers B.S, & Heaton N.S. (2019). Influenza viruses that require 10 genomic segments as antiviral therapeutics. PLoS Pathogens, 15(11), e1008098.

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Real-Time PCR Protocol for Quantitative Gene Expression Analysis

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Tissues were homogenized using metal beads in a BeadBlaster 24 Microtube Homogenizer (Benchmark Scientific) with an RLT lysis buffer (Qiagen). Cultured cells were also harvested and lysed by using RTL lysis buffer. RNA was extracted by using RNeasy Mini kits (Qiagen) and used for cDNA synthesis with an iScript Reverse Transcription kit (Bio-Rad). cDNA was amplified in a 10 μL reaction mixture containing 5 μL of iTaq SYBR Green Supermix (Bio-Rad) and 5 μM each of gene-specific forward and reverse primers. The PCR assays were denatured for 30s at 95°C, followed by 40 cycles of 15s at 95°C, and 60s at 60°C, by utilizing the CFX96 Touch real-time PCR detection system (Bio-Rad). Relative quantitation of mRNA expression was calculated using the 2^−ΔΔCt method. The primers are listed in Supplementary Table 1.

Liang Y., A.d.i.t.i., Onyoni F., Wang H., Gonzales C., Sunyakumthorn P., Wu P., Samir P, & Soong L. (2023). Brain transcriptomics reveal the activation of neuroinflammation pathways during acute Orientia tsutsugamushi infection in mice. Frontiers in Immunology, 14, 1194881.

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