Serum klotho and cardiac troponin I were measured according to the manufacturer’s protocol using Rat Klotho ELISA Kit (ABclonal, Woburn, MA, USA) and Rat Cardiac Troponin I ELISA Kit (Abcam, Cambridge, UK). 96-well microplates were analyzed with SPECTROstar Nano microplate reader (BMG Labtech, Ortenberg, Germany) at 450 nm with 650 nm as reference.
Rat cardiac troponin 1 elisa kit
Manufactured by Abcam
Sourced in United States, United Kingdom
The Rat Cardiac Troponin I ELISA Kit is a quantitative immunoassay designed for the measurement of cardiac troponin I in rat serum or plasma samples. It utilizes the sandwich ELISA technique to detect and quantify the target analyte.
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4 protocols using rat cardiac troponin 1 elisa kit
1
Quantification of Serum Klotho and Cardiac Troponin I
2
Cardiac Markers and Inflammatory Cytokines Measurement
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A rat cardiac troponin I ELISA kit (Abcam, Cambridge, United Kingdom) was used for the determination of cardiac troponin I (a marker of myocardial injury) in the serum of the rat groups according to the manufacturer’s protocol (Jin et al., 2021 (link)). In addition, a rat IL1-β ELISA kit (Abcam, Cambridge, United Kingdom) and a rat caspase-1 ELISA kit (MyBioSource, California, United States) were used for the determination of IL1-β (pro-inflammatory cytokine released as a result of NLRP3 activation) and caspase-1 (IL-1 converting enzyme), respectively, in the heart tissues, according to the manufacturer’s protocol. The colored intensity of the final product was measured at 450 nm using a microplate reader (Thermo Fisher Scientific, Waltham, MA, United States).
3
Quantitative Cardiac Injury Assessment
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At the end of the treatment, the isolated blood samples were centrifuged 3000 g for 15 minutes and the serum was frozen at –40°C until assayed for concentrations of creatine kinase MB (CKM) and cardiac troponin I (cTnI). The myocardial injury was defined by estimating CK-MB and cTnI levels in serum. Rat cardiac troponin I ELISA kit (Abcam), and rat CK-M-type ELISA kit (Abcam) were used for measurement of serum levels of cTnI and CKM.32 ,33 Both serum CK-MB and Tn I levels were quantitatively measured by means of a highly specific enzyme immunoassay according to the manufacturer's instructions and procedures.
4
Cardiac Toxicity Monitoring via TnI
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An increase in the plasma levels of TnI indicates cardiac toxicity. Although SAHA has not induced cardiac toxicity in humans, other HDAC inhibitors such as panobinostat and dacinostat have shown cardiotoxicity [56 (link),61 (link)]. Therefore, TnI was monitored following Jazz90 and Jazz167 treatment. The Rat Cardiac Troponin I ELISA kit (abcam#ab246529) was used to measure TnI levels in the plasma. The manufacturer’s instructions were followed to complete the protocol. A Biorad benchmark plus microplate spectrophotometer was used to read the OD at 450 nm.
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