Thinprep cytolyt solution

Manufactured by Hologic

Sourced in United States

The ThinPrep CytoLyt Solution is a preservative fluid used in the collection, transportation, and processing of gynecologic and non-gynecologic specimens for cytological examination. It is designed to maintain the morphological integrity of cells and prevent microbial growth during the pre-analytical stage of the diagnostic process.

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Lab products found in correlation

Tjf q190v, by Olympus (1 mentions) Tjf q180v, by Olympus (1 mentions) Cfdna urine preserve, by Streck (1 mentions) Cytorich red preservative, by BD (1 mentions) Expecttm, by Boston Scientific (1 mentions) Arietta 850, by Fujifilm (1 mentions) Gf uct260, by Olympus (1 mentions) Acquiretm, by Boston Scientific (1 mentions)

6 protocols using thinprep cytolyt solution

ERCP Sampling for Biliary Stricture Evaluation

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ERCP was performed by two experienced endoscopists using standard duodenoscopes (TJF-Q 180V or TJF-Q190V, Olympus, Hamburg, Germany) with patients under conscious sedation or general anesthesia. In all naive cases, biliary sphincterotomy was performed. Measures for the prevention of post-ERCP pancreatitis (PEP) and cholangitis were employed in accordance with available guidelines.
During ERCP, each biliary stricture was sampled twice using the cytology brush (BrushMaster V, Olympus, Hamburg, Germany) inserted over a guidewire, with at least 10 to-and-fro motions through the stricture per sampling under fluoroscopy control. Dilatation of the stricture was not routinely performed. Material from the first sampling was smeared directly onto microscopic slides for routine cytology. After the second sampling, the brush was cut and placed into 10 mL of ThinPrep CytoLyt solution (Hologic, Marlborough, MA, USA) for subsequent FISH analysis. The sampling was considered successful when the tissue sample obtained was of sufficient quantity and quality to enable both cytological and FISH analysis. Routinely, biliary plastic or fully covered self-expandable metallic stents were introduced.

Zoundjiekpon V.D., Falt P., Zapletalova J., Vanek P., Kurfurstova D., Slobodova Z., Skanderova D., Korinkova G., Skalicky P., Lovecek M, & Urban O. (2023). Fluorescence In Situ Hybridization in Primary Diagnosis of Biliary Strictures: A Single-Center Prospective Interventional Study. Biomedicines, 11(3), 755.

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Specimen Collection for Biliary Cytology and Genomics

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Samples were obtained during the ERCP procedure and included cytological brushings, forceps biopsies or both. For cytological brushes, samples were collected with multiple to-and-fro motions at the site of the stricture. The brush used for cytopathological examination and was placed into 15 mL of ThinPrep CytoLyt solution (Hologic, Marlborough, Massachusetts, USA) and transferred for specimen processing within 24 hours. A separate brush was used for BiliSeq testing and placed into a collection vial containing a detergent-based DNA lysis buffer. The collection vial was then transported to the UPMC MGP laboratory within 24 hours and processed accordingly. A similar approach was used for forceps biopsies with separate biopsies submitted for pathological examination and BiliSeq testing. No minimum specimen cellularity was required for testing. All specimens whether a brushing or biopsy were processed the same.

Singhi A.D., Nikiforova M.N., Chennat J., Papachristou G.I., Khalid A., Rabinovitz M., Das R., Sarkaria S., Ayasso M.S., Wald A.I., Monaco S.E., Nalesnik M., Ohori N.P., Geller D., Tsung A., Zureikat A.H., Zeh H., Marsh J.W., Hogg M., Lee K., Bartlett D.L., Pingpank J.F., Humar A., Bahary N., Dasyam A.K., Brand R., Fasanella K.E., McGrath K, & Slivka A. (2019). Integrating next-generation sequencing to endoscopic retrograde cholangiopancreatography (ERCP)-obtained biliary specimens improves the detection and management of patients with malignant bile duct strictures. Gut, 69(1), 52-61.

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Urinary cfDNA Preservation Protocols

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The stabilizing effect of three different preservatives was investigated: i) cfDNA Urine Preserve (Streck Inc), a commercially available preservative specifically for urinary cfDNA, ii) Urine conservation medium (UCM, University of Antwerp, Wilrijk, Belgium), an in-house developed preservative, designed to preserve human papillomavirus (HPV) DNA and total human DNA in urine samples [32 (link)] and iii) ThinPrep Cytolyt Solution (Hologic Inc, Marlborough, Massachusetts, United Stated of America), a preservative for cytological specimens.
Each sample, derived from one of the five HVs (two male and three female), was divided into five separate fractions after sample homogenization. Three fractions were supplemented with the preservatives described previously. The whole fractions were isolated. These fractions were stored at room temperature (RT) for one week. One fraction without preservative was also stored at RT for one week. The other fraction without preservative was processed immediately (<2h) after collection and served as a reference. Urine samples from three cancer patients (one male and two female) were also included in this study. Due to the limited volume of the patient samples (60–75 mL) and the volume requirements for the preservatives (Streck preservative requires ≥25 mL urine), not all testing conditions could be evaluated (Tables 2 and 3).

Augustus E., Van Casteren K., Sorber L., van Dam P., Roeyen G., Peeters M., Vorsters A., Wouters A., Raskin J., Rolfo C., Zwaenepoel K, & Pauwels P. (2020). The art of obtaining a high yield of cell-free DNA from urine. PLoS ONE, 15(4), e0231058.

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Preservative Evaluation for Molecular Analysis

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As the first step after sample collection, several preservatives combined with a 72-hour delay in processing were evaluated on their compatibility with molecular analyses. In total, 6 collection media were selected based on literature review,^{8-10 (link)} a questionnaire data set, and considerations based on the new European regulation 2017/746 on in vitro diagnostics medical devices—namely, PBS, formaldehyde 4% buffered solution (formalin; Chem-Lab), ThinPrep CytoLyt Solution (Hologic), CytoRich Red preservative (Becton Dickinson), and ethanol solutions of 40% and 95% ethanol (EtOH40% and EtOH95%, respectively). The 72-hour delay in processing was selected to allow for samples left over the weekend.
We added 1 mL of collection medium to the cell pellet. Samples were left at room temperature for 72 hours before centrifugation for 5 minutes at 450g. The supernatant was removed, and the cell pellet was resuspended in PBS. Samples were centrifuged again, and the supernatant was removed. The cell pellet was snap-frozen in liquid nitrogen and stored at –80°C until nucleic acid isolation and analysis. All samples were processed in duplicate. Additionally, reference samples, consisting of cell pellets immediately snap-frozen after cell collection, were included.

Sorber L., Claes B., Zwaenepoel K., Van Dorst B., De Winne K., Fransen E., Wener R., Lapperre T., Lardon F, & Pauwels P. (2021). Evaluation of Cytologic Sample Preparations for Compatibility With Nucleic Acid Analysis. American Journal of Clinical Pathology, 157(2), 293-304.

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EUS-FNA for Pancreatic Lesion Diagnosis

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Patients underwent EUS-FNA using a GF-UCT260 linear echoendoscope (Olympus Medical, Tokyo, Japan) connected to an ultrasound scanning system (ARIETTA 850; Fujifilm, Tokyo, Japan). The pancreatic lesion was punctured using a 22/25G aspiration needle (Expect^TM or Acquire^TM; Boston Scientific, Natick, MA, USA; or EZ Shot3^TM; Olympus Medical, Tokyo, Japan) under real-time ultrasound guidance. The stylet was withdrawn and aspirated (10 cc negative pressure) using the attached syringe and the aspiration needle was moved back and forth 20 times within the lesion before being withdrawn from the echoendoscope. Finally, the aspirated material was pushed out into a preservative liquid (ThinPrep CytoLyt Solution; Hologic, Marlborough, MA, USA) by reinsertion of the stylet.
The aspirated material was separated for histological evaluation, cytological evaluation and Kras mutation analysis. The solid materials were fixed in 10% formalin, embedded in paraffin and sliced thinly for additional immunostaining as required. The liquid material was treated using the ThinPrep^® method according to the manufacturer’s recommendations and then evaluated immediately by Papanicolaou staining. The residual LBC specimens were stored at 4 °C until DNA extraction.

Itonaga M., Ashida R., Murata S.I., Yamashita Y., Hatamaru K., Tamura T., Kawaji Y., Kayama Y., Emori T., Kawai M., Yamaue H., Matsuzaki I., Nagai H., Kinoshita Y., Wan K., Shimokawa T, & Kitano M. (2022). Kras Gene Analysis Using Liquid-Based Cytology Specimens Predicts Therapeutic Responses and Prognosis in Patients with Pancreatic Cancer. Cancers, 14(3), 551.

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Buccal Epithelium Sampling and DNA Extraction

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All patient recruitment and sample collection was performed with the appropriate human subjects protocol approval from the Institutional Review Board at the Albert Einstein College of Medicine. We have described the collection of these samples previously (39 (link)). We collected buccal epithelium using exfoliative brushing. The brushes were stored in 15 ml Falcon tubes containing 4 ml of ThinPrep CytoLyt Solution (Hologic, Inc.) immediately after the swabbing. The buccal epithelial cells were separated from the brush by shaking, and then spun down to remove the preservative solution. The pellets were stored at −80°C. DNA from the buccal epithelial samples was extracted with a modification of the protocol for the Qiagen Gentra Puregene Buccal Cell kit (QIAGEN) (39 (link)).

Li Q., Suzuki M., Wendt J., Patterson N., Eichten S.R., Hermanson P.J., Green D., Jeddeloh J., Richmond T., Rosenbaum H., Burgess D., Springer N.M, & Greally J.M. (2015). Post-conversion targeted capture of modified cytosines in mammalian and plant genomes. Nucleic Acids Research, 43(12), e81.

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