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Anti β catenin 6b3

Manufactured by Cell Signaling Technology
Sourced in United States

The Anti-β-Catenin (6B3) is a monoclonal antibody that specifically binds to β-catenin, a key protein involved in the Wnt signaling pathway. This antibody can be used for the detection and analysis of β-catenin in various experimental applications.

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2 protocols using anti β catenin 6b3

1

Western Blot Analysis of Hippocampal Proteins

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Hippocampal tissue was homogenized and Western blot analysis was carried out as previously described.22 The following primary antibodies were applied: anti-phospho-GSK-3βSer9 (5B3; Cell Signaling Technology, Danvers, MA, USA; #9323); anti-total GSK-3β (27C10; Cell Signaling #9315); anti-β-Catenin (6B3; Cell Signaling #9582); anti-βIII Tubulin (Promega, Madison, WI, USA; #G7121); and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 6C5; EMD Millipore, Etobicoke, ON, Canada, #MAB374).
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2

Western Blot Analysis of Cell Markers

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The following antibodies were used for western blot analysis: anti‐CA2 (Abcam, #ab124687, 1:1000), anti‐β‐catenin (6B3) (Cell Signaling Technology, #9582, 1:1000), anti‐N‐cadherin (Cell Signaling Technology, #4061, 1:1000), anti‐vimentin (Cell Signaling Technology, #5741, 1:1000), HRP‐conjugated secondary goat anti‐rabbit antibody (Santa Cruz Biotechnology, #sc‐2004, 1:10,000), and HRP‐conjugated anti‐β‐actin (Santa Cruz Biotechnology, #sc‐47,788, 1:100,000). Details are provided in Data S1.
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