24 well tissue plates

The ability of microbial cells to adhere to the intestinal lining was determined using HT-29 colonic carcinoma cells derived from the human small intestine, as adopted from a previous study [40 ], with slight modifications. Monolayers of HT-29 cells were prepared in DMEM medium (Sigma, USA) supplemented with 10% fetal bovine solution (FBS) (Sigma, USA) in 24-well tissue plates (BD Biosciences, San Jose, CA USA) at a concentration of 1 x 10⁵ cells/well. The cells were incubated at 37°C for 2 hrs together with 2 x 10⁷ CFU/mL of a cultured strain to test for adhesion. After incubation, the HT-29 cells were aspirated and washed three times with 1 X PBS to remove unbound microbial cells. Adherent cells were detached, and appropriate dilution series were prepared followed by enumeration of viable colonies on appropriate agar plates in triplicate.

The ability of microbial cells to adhere to the intestinal lining was determined using HT-29 colonic carcinoma cells derived from the human small intestine, according to a previous report with slight modifications (Kim et al., 2019 (link)). Monolayers of HT-29 cells were prepared in DMEM (Sigma, United States) supplemented with 10% fetal bovine solution (FBS) (Sigma, United States) in 24-well tissue plates (BD Biosciences, San Jose, CA, United States) at a concentration of 1 × 10⁵ cells/well. To test the abilities of the strains for adhesion to host cells, HT-29 cells were incubated with 2 × 10⁷ CFU/mL of a cultured strain for 2 h at 37°C with 5% CO₂. After incubation, the HT-29 cells were aspirated and washed three times with 1 × PBS to remove unbound microbial cells. Adherent cells were detached and appropriate dilution series were prepared, followed by enumeration of viable colonies on appropriate agar plates in triplicate.