Live video tracking system

The Open field tests were performed as described earlier (60 (link), 61 (link)) with slight modifications and used to assess spontaneous exploratory activity. Briefly, each mouse was allowed to freely explore an open field arena for 5 min. The testing apparatus was a classic open field (i.e., a wooden floor square arena, 40 × 40 cm, with walls 30 cm high). A video camera (Basler Gen I Cam - Basler acA 1300–60) connected to a Noldus computer system was placed above the box. Each mouse was placed individually on the center of the arena and the performance was monitored by the live video tracking system (Noldus System). Exploratory parameters in OFT that were analyzed include total distance traveled by the mice during 5 min of free exploration in the open field arena and the velocity with which this distance was covered.

It was performed as described earlier (Patel et al., 2019 (link); Patel et al., 2020 ). Briefly, each mouse was allowed to freely explore an open field arena for 5 min. The testing apparatus was a classic open field (i.e., a wooden floor square arena, 40 × 40 cm, with walls 30 cm high). A video camera (Basler Gen I Cam-Basler acA 1300–60) connected to a Noldus computer system was placed above the box. Each mouse was placed individually on the center of the arena and the locomotor activity and other parameters like velocity, total distance travelled and center time frequency was monitored for 5 minutes using live video tracking system (Noldus System). The central area was arbitrarily defined as a square of 20 × 20 cm (half the total area).

Open Field test was performed as described earlier (Ghosh et al., 2007 (link); Ghosh et al., 2009 (link)) with slight modifications and used to assess spontaneous exploratory activity and stress-related behaviors in open environment. Briefly, each mouse was allowed to freely explore an open field arena for 5 min. The testing apparatus was a classic open field (i.e., a wooden floor square arena, 40 × 40 cm, with walls 30 cm high). A video camera (Basler Gen I Cam - Basler acA 1300–60) connected to a Noldus computer system was placed above the box. Each mouse was placed individually on the center of the arena and the locomotor activity and stereotypical behaviors like rearing and grooming were monitored for 5 min using live video tracking system (Noldus System). The central area was arbitrarily defined as a square of 20 × 20 cm (half the total area).