Liquid chromatography-mass spectrometry (LC/MS) measurement and analysis were performed according to a previously described methods [10 (link),11 (link)]. The carbohydrate concentration was measured following the method reported by Suzuki et al. [12 (link)]. Euglena extract was treated with exo-1,3-β-D-glucanase (Neogen Chemicals Ltd., MI, USA.) or β-1,3-glucanase (Thermo Stable Enzyme Laboratory Co., Ltd., Kobe, Japan). Polyvinylpolypyrrolidone (PVPP; Sigma-Aldrich, St. Louis, MO, USA) treatment was used to remove polyphenols from Euglena extract. The chelating agents ethylenediaminetetraacetic acid (EDTA; FUJIFILM Wako, Osaka, Japan) and ethylene glycol tetraacetic acid (EGTA; FUJIFILM Wako, Osaka, Japan) were added to trap metal ions. A cation exchange resin (CER; GL sciences Inc., Tokyo, Japan) was used to remove metal ions from Euglena extract. The samples were treated twice to remove approximately 99.4% zinc. The zinc concentration was determined using a zinc assay kit (Metallogenics, Tokyo, Japan), according to the manufacturer’s instructions.
Ethylenediaminetetraacetic acid edta
Manufactured by Fujifilm
Sourced in Japan
Ethylenediaminetetraacetic acid (EDTA) is a chemical compound used as a laboratory reagent. It is a chelating agent, capable of binding to metal ions. EDTA is commonly used in various analytical and diagnostic procedures to control the concentration of metal ions in solutions.
Automatically generated - may contain errors
Lab products found in correlation
Ecl western blotting detection reagent, by GE Healthcare (2 mentions)
Polyvinylpolypyrrolidone pvpp, by Merck Group (1 mentions)
Morpholinepropanesulfonic acid, by Merck Group (1 mentions)
P nitrophenyl butyrate p npb, by Merck Group (1 mentions)
Viscozyme, by Novozymes (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Trypan blue, by Nacalai Tesque (1 mentions)
Aprotinin, by Fujifilm (1 mentions)
Paraformaldehyde (pfa), by Fujifilm (1 mentions)
Tissue tek oct compound, by Sakura Finetek (1 mentions)
Rabbit polyclonal antibodies, by Santa Cruz Biotechnology (1 mentions)
Nitrocellulose membrane, by GE Healthcare (1 mentions)
2 7 dichlorofluorescein diacetate dcf da, by Thermo Fisher Scientific (1 mentions)
Srebp2, by Santa Cruz Biotechnology (1 mentions)
Srebp1, by Santa Cruz Biotechnology (1 mentions)
Phosphor ampkα, by Cell Signaling Technology (1 mentions)
Protease inhibitor mixture, by Fujifilm (1 mentions)
2 7 dichlorofluorescein diacetate dcfh da, by Thermo Fisher Scientific (1 mentions)
L alliin, by Merck Group (1 mentions)
Trans ferulic acid, by Merck Group (1 mentions)
6 protocols using ethylenediaminetetraacetic acid edta
1
Euglena Metabolite Analysis by LC/MS
2
Porcine Pancreatic Lipase Inhibition
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Porcine pancreatic lipase (Type II), orlistat, morpholinepropanesulfonic acid (MOPS), Tris-HC1, p-nitrophenyl butyrate (p-NPB), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis-diammonium salt (ABTS) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Ethylenediaminetetraacetic acid (EDTA) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Viscozyme® was purchased from Novozymes (Denmark). All other reagents were of biochemical grade.
3
Isolation and Characterization of Primary Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HA was purchased from Kikkoman Biochemifa Company (Tokyo, Japan). PTH was supplied from CosMED Pharmaceutical Co., Ltd. (Kyoto, Japan). The SEP-Column containing 200 mg C18 was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA, USA). Trypan blue was purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Tissue-TeK® O.C.T. Compound was purchased from Sakura Finetek Japan Co., Ltd. (Tokyo, Japan). Paraformaldehyde, ethylenediaminetetraacetic acid (EDTA), and aprotinin were purchased from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). All other chemicals were obtained commercially as reagent-grade products.
4
Protease Inhibitor Mixture Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The protease inhibitor mixture ethylenediaminetetraacetic acid (EDTA) was purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 2′,7′-Dichlorofluorescein diacetate (DCF-DA) was obtained from Molecular Probes (Eugene, OR, USA). Rabbit polyclonal antibodies against peroxisome proliferator-activated receptor α (PPARα), sterol regulatory element binding protein-1 (SREBP-1) and sterol regulatory element binding protein-2 (SREBP-2), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), and mouse monoclonal antibodies against fatty acid synthase (FAS), β-actin, and histone, and goat polyclonal antibody against mitochondrial uncoupling protein 2 (UCP-2) and stearoyl-CoA desaturase-1 (SCD-1) were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit polyclonal antibodies against acetyl-CoA carboxylase (ACC), AMPKα, and phosphor-AMPKα were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). ECL Western Blotting Detection Reagents and nitrocellulose membranes were supplied by GE Healthcare (Buckinghamshire, UK).
5
Antioxidant and Anti-inflammatory Assays of Allium hookeri
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
(±)-L-Alliin, trans-ferulic acid and chlorogenic acid (Fig. 1 ) were purchased from Sigma-Aldrich (St. Louis, USA). Diethylenetriaminepentaacetic acid (DTPA), dihydrorhodamine 123 (DHR 123), and phenylmethylsulfonyl fluoride (PMSF) were obtained from Sigma-Chemical Co. (St. Louis, MO, USA). 2’,7’-Dichlorofluorescein diacetate (DCFH-DA) was obtained from Molecular Probes (Eugene, OR, USA). The protease inhibitor mixture and ethylenediaminetetraacetic acid (EDTA) were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). BCA protein assay kit (bicinchoninic acid) was obtained from Thermo Scientific (Rockford, IL, USA). Rabbit polyclonal antibodies against superoxide dismutase (SOD), catalase, heme oxygenase-1 (HO-1) and NF-кBp65, goat polyclonal antibodies against tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and mouse monoclonal antibodies against β-actin and histone were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Goat anti-rabbit and goat anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP)-conjugated secondary antibodies were acquired from Santa Cruz Biotechnology, Inc. ECL Western Blotting Detection Reagents were supplied by GE Healthcare (Piscataway, NJ, USA).![]()
Identification of chemical ingredients of root extract of Allium hookeri
6
Fructose-Induced Metabolic Changes in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In experiment-I, mice were administered fructose (Nacalai Tesque, Kyoto, Japan) solution (2 g/kg body weight) intragastrically after 14 h fasting. The mice were sacrificed at 5 and 30 min after fructose administration. Control mice received glucose (Wako Pure Chemical Industries, Osaka, Japan) solution (2 g/kg body weight), instead of fructose.
In experiment-II, mice were administered fructose solution in the same manner as in experiment-I. The mice were sacrificed at 1–5 h after fructose administration. Blood samples were collected from the orbital sinus under anaesthesia (isoflurane) [23 (link)]. Ethylenediaminetetraacetic acid (EDTA) (Wako Pure Chemical Industries, Osaka, Japan) was used as an anticoagulant. After blood sampling, mice were sacrificed by cervical dislocation, and tissue samples were collected. Plasma and tissue samples were stored at −80 °C until analysis.
In experiment-II, mice were administered fructose solution in the same manner as in experiment-I. The mice were sacrificed at 1–5 h after fructose administration. Blood samples were collected from the orbital sinus under anaesthesia (isoflurane) [23 (link)]. Ethylenediaminetetraacetic acid (EDTA) (Wako Pure Chemical Industries, Osaka, Japan) was used as an anticoagulant. After blood sampling, mice were sacrificed by cervical dislocation, and tissue samples were collected. Plasma and tissue samples were stored at −80 °C until analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!