AICAR was from Toronto Research Chemicals. A769662 was from Selleck Chemicals. Compound 13 was obtained as previously described (18 (link)). 991 (5-{[6-chloro-5-(1-methylindol-5-yl)-1H-benzimidazol-2-yl]oxy}-2-methyl-benzoic acid; CAS no. 129739-36-2) as obtained as previously described (7 (link)). Protein G Sepharose was from GE Healthcare, and FLAG-M2 resin from Sigma-Aldrich. ECL reagent and P81 filter papers were obtained from GE Healthcare. [γ-32P]-ATP was from Perkin-Elmer. AMARA, LKBtide, and Sakamototide substrate peptides were synthesised by GL Biochem. The COS1 cell line was obtained from American Type Culture Collection, and the C2C12 cell line was obtained from Sigma-Aldrich. Frozen tissues or extracts from AMPKα1-/α2-, AMPKβ1-/β2-, and AMPKγ3-deficient mice were obtained from Benoit Viollet and Marc Foretz (Institut National de la Santé et de la Recherche Médicale, Institut Cochin, Paris, France), Gregory Steinberg (McMaster University, Hamilton, ON, Canada), and Alexander Chibalin and Juleen Zierath (Karolinska Institutet, Stockholm, Sweden), respectively. All cell culture reagents were purchased from Thermo Fisher Scientific, and all other chemicals were from Sigma-Aldrich unless otherwise stated.
C2c12 cell line
Manufactured by Merck Group
Sourced in United States
The C2C12 cell line is a well-established murine skeletal muscle cell line derived from the thigh muscle of a C3H mouse. This cell line is commonly used in research as a model for the study of muscle cell differentiation and development.
Automatically generated - may contain errors
Lab products found in correlation
Ecl reagent, by GE Healthcare (1 mentions)
Aicar, by LGC (1 mentions)
Sakamototide, by GL Biochem (1 mentions)
Flag m2 resin, by Merck Group (1 mentions)
A769662, by Selleck Chemicals (1 mentions)
Protein g sepharose, by GE Healthcare (1 mentions)
γ 32p atp, by PerkinElmer (1 mentions)
Glutamine, by Merck Group (1 mentions)
Pen strep, by Merck Group (1 mentions)
Dmem paa, by GE Healthcare (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Mc3t3 e1 cell line, by American Type Culture Collection (1 mentions)
2 protocols using c2c12 cell line
1
Studying AMPK Regulation and Signaling
2
Cell Culture Protocols for Osteoblast and Myoblast Research
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The MC3T3-E1 cell line was obtained from the American Type Culture Collection (LGC Standards S.r.L., Sesto S. Giovanni, MI, Italy) and cultured in Dulbecco's modified Eagle'a medium (DMEM, PAA; GE Healthcare, Uppsala, Sweden), 10% fetal bovine serum (FBS; Gibco, ThermoFisher, Waltham, MA, USA), 1% penicillin and streptomycin (Penstrep; Sigma-Aldrich, St. Louis, MO, USA) and 1% glutamine (Sigma-Aldrich, St. Louis, MO, USA) as subculture medium. For gene expression assays, cells were plated on polished or SLA surfaces at the density of 30,000 cells/well in 24-well plates, in triplicate. After 24 hours, the subculture medium was replaced with subculture medium added with 250 μM ascorbic acid (Sigma Chemicals, St. Louis, MO, USA).
The C2C12 cell line was obtained from the European Catalog of Cell Cultures (Health Protection Agency Culture Collections, Salisbury, UK) and grown in subculture medium. For reporter assays, C2C12 cells were plated on titanium surfaces in OptiMEM (Invitrogen, San Giuliano Milanese, MI, Italy), 5% FBS, 1% Penstrep at the density of 125,000 cells/well, and the cells were assayed after 24 hours. C2C12 is a popular cell line to study Wnt canonical signaling because of the abundance of molecular machinery for this pathway.
The C2C12 cell line was obtained from the European Catalog of Cell Cultures (Health Protection Agency Culture Collections, Salisbury, UK) and grown in subculture medium. For reporter assays, C2C12 cells were plated on titanium surfaces in OptiMEM (Invitrogen, San Giuliano Milanese, MI, Italy), 5% FBS, 1% Penstrep at the density of 125,000 cells/well, and the cells were assayed after 24 hours. C2C12 is a popular cell line to study Wnt canonical signaling because of the abundance of molecular machinery for this pathway.
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