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The HP1BP3 is a laboratory instrument designed for general-purpose applications. It features a compact and durable construction to support routine usage in a laboratory setting.

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Lab products found in correlation

Lsm 880 confocal laser scanning microscope, by Zeiss (2 mentions) Human igg, by Jackson ImmunoResearch (2 mentions) Rabbit polyclonal anti sap polyclonal antibody, by Thermo Fisher Scientific (2 mentions) Rhodamine red x conjugated goat anti rabbit secondary, by Jackson ImmunoResearch (2 mentions) Rabbit anti human igg, by Agilent Technologies (2 mentions) Ultimate orf clone, by Thermo Fisher Scientific (1 mentions)

3 protocols using hp1bp3

1

CRISPR gRNA Design and Cloning for LoF/GoF Studies

Cited 1 time
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All gRNAs used for the in vivo barcode enrichment competition assay LoF screening were designed using CRISPOR (http://crispor.tefor.net/crispor.py). The gRNAs were generated with barcode sequences through a previously described HiTMMoB approach in a PB (PBCAG) vector by gateway cloning (1 (link)). For individual LoF studies with ZC3H7B, DMBT1, and HP1BP3 gRNAs, each gRNA was generated in a Px330-Crispr-mCherry vector by gateway cloning (ZC3H7B, 5′-GGTCGTTGCCCTCGGCAAAC-3′; DMBT1, 5′-CTCCACAGGCCACGTCATCC-3′; and HP1BP3, 5′-GAATTCACGGCTCGACGTAT-3′). For individual GoF studies, the ZC3H7B ORF was obtained from Origin (catalog no. RC219566). The DMBT1 and HP1BP3 ORFs were obtained from Thermo Fisher Scientific (Ultimate ORF clones). The efficiency of individual vectors was confirmed by staining the brain (LoF vectors) and NIH3T3 cells (GoF vectors) (fig. S4).
Choi D.J., Armstrong G., Lozzi B., Vijayaraghavan P., Plon S.E., Wong T.C., Boerwinkle E., Muzny D.M., Chen H.C., Gibbs R.A., Ostrom Q.T., Melin B., Deneen B., Bondy M.L., Bainbridge M.N., Amos C.I., Barnholtz-Sloan J.S., Bernstein J.L., Claus E.B., Houlston R.S., Il'yasova D., Jenkins R.B., Johansen C., Lachance D., Lai R., Melin B.S., Merrell R.T., Olson S.H., Sadetzki S., Schildkraut J., Shete S., Ambrose J.C., Arumugam P., Bevers R., Bleda M., Boardman-Pretty F., Boustred C.R., Brittain H., Brown M.A., Caulfield M.J., Chan G.C., Giess A., Griffin J.N., Hamblin A., Henderson S., Hubbard T.J., Jackson R., Jones L.J., Kasperaviciute D., Kayikci M., Kousathanas A., Lahnstein L., Lakey A., Leigh S.E., Leong I.U., Lopez F.J., Maleady-Crowe F., McEntagart M., Minneci F., Mitchell J., Moutsianas L., Mueller M., Murugaesu N., Need A.C., O'Donovan P., Odhams C.A., Patch C., Perez-Gil D., Pereira M.B., Pullinger J., Rahim T., Rendon A., Rogers T., Savage K., Sawant K., Scott R.H., Siddiq A., Sieghart A., Smith S.C., Sosinsky A., Stuckey A., Tanguy M., Taylor Tavares A.L., Thomas E.R., Thompson S.R., Tucci A., Welland M.J., Williams E., Witkowska K., Wood S.M, & Zarowiecki M. (2023). The genomic landscape of familial glioma. Science Advances, 9(17), eade2675.
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2

Immunofluorescence Analysis of SAP and IgG in MGMID

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Formalin-fixed paraffin-embedded sections, cut at 3 μm, were deparaffinized, and antigen retrieval was performed at 99° C. The sections were reacted with rabbit polyclonal anti-SAP polyclonal antibody (1:400; ThermoFisher, Waltham, MA) followed by a Rhodamine red X-conjugated goat anti-rabbit secondary which was solid-phase adsorbed to ensure minimal cross-reaction with human IgG (1:100; Jackson ImmunoResearch Laboratories, West Grove, PA). Each case was run with positive and negative controls. The stain was evaluated by standard immunofluorescence microscopy. It was judged to be positive if there was positive granular capillary loop staining in the glomeruli, and negative if there was no capillary loop staining in the glomeruli. Colocalization of IgG and SAP in the glomerular basement membranes was examined by confocal microscopy using a Zeiss LSM 880 confocal laser scanning microscope (Zeiss Microscopy, Jena, Germany). For this analysis, polyclonal (fluorescein isothiocyanate–conjugated) rabbit anti-human IgG (1:40; Agilent, Santa Clara, CA) was reacted with the heat retrieved tissue following the staining for SAP as described above. Negative controls were performed to ensure antibody specificity by omitting primary antibodies. Four cases of MGMID were tested for the presence of HP1BP3 (1:100; Thermo Fisher, Waltham, MA) by immunoperoxidase staining.
Larsen C.P., Sharma S.G., Caza T.N., Kenan D.J., Storey A.J., Edmondson R.D., Herzog C, & Arthur J.M. (2019). Serum amyloid P deposition is a sensitive and specific feature of membranous-like glomerulopathy with masked IgG kappa deposits. Kidney international, 97(3), 602-608.
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3

Immunofluorescence Analysis of SAP and IgG in MGMID

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed paraffin-embedded sections, cut at 3 μm, were deparaffinized, and antigen retrieval was performed at 99° C. The sections were reacted with rabbit polyclonal anti-SAP polyclonal antibody (1:400; ThermoFisher, Waltham, MA) followed by a Rhodamine red X-conjugated goat anti-rabbit secondary which was solid-phase adsorbed to ensure minimal cross-reaction with human IgG (1:100; Jackson ImmunoResearch Laboratories, West Grove, PA). Each case was run with positive and negative controls. The stain was evaluated by standard immunofluorescence microscopy. It was judged to be positive if there was positive granular capillary loop staining in the glomeruli, and negative if there was no capillary loop staining in the glomeruli. Colocalization of IgG and SAP in the glomerular basement membranes was examined by confocal microscopy using a Zeiss LSM 880 confocal laser scanning microscope (Zeiss Microscopy, Jena, Germany). For this analysis, polyclonal (fluorescein isothiocyanate–conjugated) rabbit anti-human IgG (1:40; Agilent, Santa Clara, CA) was reacted with the heat retrieved tissue following the staining for SAP as described above. Negative controls were performed to ensure antibody specificity by omitting primary antibodies. Four cases of MGMID were tested for the presence of HP1BP3 (1:100; Thermo Fisher, Waltham, MA) by immunoperoxidase staining.
Larsen C.P., Sharma S.G., Caza T.N., Kenan D.J., Storey A.J., Edmondson R.D., Herzog C, & Arthur J.M. (2019). Serum amyloid P deposition is a sensitive and specific feature of membranous-like glomerulopathy with masked IgG kappa deposits. Kidney international, 97(3), 602-608.
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