1 phenyl 3 methyl 5 pyrazolone pmp

Monosaccharide standards, including Arabinose (Ara), Fucose (Fuc), Galactose (Gal), Galacturonic acid (GalA), glucose (Glc), Glucuronic acid (GlcA), Mannose (Man), Rhamnose (Rha), and Xylose (Xyl), of chromatographic purity were purchased from Shanghai Yuanye Bio-Technology Co. (Shanghai, China). Anhydrous ethanol, glucose, phenol, sulfuric acid, and 1-Phenyl-3-methyl-5-pyrazolone (PMP) of analytical purity were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). Acetonitrile of chromatographic purity was purchased from Tianjin Chemical Reagent Co. (Tianjin, China). coomassie brilliant blue G250 of analytical purity purchased from Beijing Dingguo Bio (Beijing, China). Pancreatic peptone, yeast extract powder, beef extract, and biochemical reagent were purchased from Beijing Auboxing Biotechnology Co. (Beijing, China). Bovine serum albumin of biotechnology grade was purchased from Shanghai Maclean Biochemical Technology Co. (Shanghai, China). Pure water was used during the experiments.

Acetonitrile and formic acid (HPLC grade) were purchased from Fisher Scientific (Fairlawn, NJ, USA). Methanol (HPLC grade) was purchased from Merck (Darmstadt, Germany). Water was purified by a MilliQ system (Millipore, Milford, MA, USA). 1-Phenyl-3-methyl-5-pyrazolone (PMP) was provided by Sinopharm (Beijing, China). The reference samples for saccharides used for the assays were stachyose (Sta), raffinose (Raf), melibiose (Mel), arabinose (Ara), mannose (Man) (Shanghai source leaves Biological Co., Ltd., Shanghai, China), galactose (Gal), glucose (Glu), and manninotriose (Mann) (Sichuan Wei Keqi Biological Co., Ltd., Chengdu, China).