Prolong gold anti fade mountant with dapi p36931

Imatinib (Gleevec; STI-571) was purchased from Santa Cruz Biotechnology (Dallas, Texas). Smooth muscle (SM) α-actin primary antibody was purchased from Abcam (ab5694; Cambridge, MA). Goat anti-rabbit secondary antibody (A-11037) and prolong gold anti-fade mountant with DAPI (P36931) were purchased from Life Technologies (Grand Island, NY). Normal goat serum was purchased from Sigma-Aldrich (G9023; St. Louis, MO). VITROS DT-slides were purchased from Ortho Clinical Diagnostics (Rochester, NY). All surgical tools were purchased from Roboz Surgical Instrument (Gaithersburg, MD). Isoflurane was purchased from Butler Schein Animal Health (Dublin, OH). All other chemicals were purchased from Fisher Scientific (Fair Lawn, NJ).

PIO and compound C were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). VITROS DT-slides were purchased from Ortho Clinical Diagnostics (Rochester, NY, USA). All surgical tools were purchased from Roboz Surgical Instrument (Gaithersburg, MD). The primary antibodies for phospho-AMPKα ^Thr172 (2535) and pan-AMPKα (2532) were purchased from Cell Signaling Technology (Danvers, MA, USA). The primary antibody for SM α-actin (ab5694) was purchased from Abcam (Cambridge, MA, USA). Goat anti-Rabbit IgG secondary antibody Alexa Fluor^® 594 conjugate (A-11037) and prolong gold anti-fade mountant with DAPI (P36931) were purchased from Life Technologies (Carlsbad, CA, USA). All other chemicals were from Fisher Scientific (Fair Lawn, NJ, USA) or Sigma Chemical (St. Louis, MO, USA).

Sulforaphane (SFN) was purchased from EMD Millipore (cat# 574215; Billerica, MA). Recombinant human leptin was purchased from R&D systems Inc. (cat# 398-LP; Minneapolis, MN). VITROS DT-slides were purchased from Ortho Clinical Diagnostics (Rochester, NY). All surgical tools were purchased from Roboz Surgical Instrument (Gaithersburg, MD). The primary antibody for smooth muscle α-actin was purchased from Abcam (cat# ab5694; Cambridge, MA). The primary antibody for Ki-67 was purchased from Thermo Scientific (cat# RM-9106-S1; Hanover, IL). The primary antibodies for cyclin D1, phospho-Akt (Ser473), phospho-ribosomal protein S6 (Ser235/236) and phospho-p70S6kinase (Thr389) were purchased from Cell Signaling Technology (Danvers, MA). Goat anti-rabbit secondary antibody (A-11037) and prolong gold anti-fade mountant with DAPI (P36931) were purchased from Life Technologies (Grand Island, NY). All other chemicals were from Fisher Scientific (Fair Lawn, NJ) or Sigma-Aldrich (St. Louis, MO).