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Ki8751

Manufactured by R&D Systems
Sourced in United Kingdom

The Ki8751 is a laboratory equipment product designed for research and development purposes. It serves as a core tool for researchers and scientists in their work. The product's primary function is to provide a reliable and accurate platform for conducting specific experiments and analyses. No further details on the intended use or applications of the Ki8751 are available at this time.

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3 protocols using ki8751

1

Mouse Angiogenesis Assay Protocol

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C57BL/6 mice were purchased from Jackson Laboratory (Bar Harbor, ME). All animal breeding and procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Stanley Manne Children’s Research Institute/Northwestern University. Animals received humane care in compliance with the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85Y23, revised 1996). Anti-CD31 (#ab28364) and anti-BrdU (#ab6326) antibodies were purchased from Abcam (Cambridge, MA), anti-VEGF-A (#sc-7269) antibodies from Santa Cruz Biotechnology (Santa Cruz, CA) and Horseradish peroxidase (HRP)-conjugated antibodies (#A11037 and #A11007) from Life Technologies (Grand Island, NY). DMOG was purchased from Sigma-Aldrich (St. Louis, MO) and Ki8751 from R&D Systems (Minneapolis, MN).
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2

Mouse Angiogenesis Assay Protocol

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C57BL/6 mice were purchased from Jackson Laboratory (Bar Harbor, ME). All animal breeding and procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Stanley Manne Children’s Research Institute/Northwestern University. Animals received humane care in compliance with the Guide for the Care and Use of Laboratory Animals (NIH Publication No. 85Y23, revised 1996). Anti-CD31 (#ab28364) and anti-BrdU (#ab6326) antibodies were purchased from Abcam (Cambridge, MA), anti-VEGF-A (#sc-7269) antibodies from Santa Cruz Biotechnology (Santa Cruz, CA) and Horseradish peroxidase (HRP)-conjugated antibodies (#A11037 and #A11007) from Life Technologies (Grand Island, NY). DMOG was purchased from Sigma-Aldrich (St. Louis, MO) and Ki8751 from R&D Systems (Minneapolis, MN).
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3

Src-Akt-mTOR Signaling Pathway Monitoring

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Antibodies used were: anti-p-Src Y416, anti-Src 36D10, anti-LC3B, anti-FAK, anti-p-PDK1 S241, anti-PDK1, anti-p-Akt S473, anti-Akt, anti-GAPDH, anti-p-mTOR S2448, anti-p-mTOR S2481, anti-mTOR, anti-p-S6 S235/236, anti-p-S6 S240/244, anti-S6, anti-p-p70S6K T389, anti-p70S6K, anti-mouse and anti-rabbit IgG-peroxidase conjugated secondary antibodies (all New England Biolabs, Herts, UK), anti-LC3 (MBL, MA, USA), and anti-paxillin (BD Transduction Laboratories, Oxford, UK). Alexa 488 and Alexa 594 conjugated secondary antibodies, Deep Red Cell Mask and Vectashield microscope mounting medium with DAPI were from Invitrogen (Paisley, UK).
Tocriscreen Kinase Inhibitor Toolbox, SB218078, SB216763, ZM306416, PD407824, BIO, Ki8751 and PF4708671 were all from R & D (Abington, UK), hygromycin B from Merck Biosciences (Nottingham, UK), dasatinib from Bristol Myers Squibb (Middlesex, UK) and 3MA from Sigma (Poole, UK). The Micro BCA Protein Assay Kit was from Pierce Ltd. (Northumbria, UK), Lipofectamine2000 from Invitrogen (Paisley, UK) and HiPerFect from Qiagen (Crawley, UK). siRNA was from Dharmacon (Colorado, USA).
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