Chicken anti vimentin polyclonal

Primary antibodies used in this study were mouse anti-human Ki67 (1∶200, BD Biosciences), chicken anti-Vimentin polyclonal (1∶1000, Millipore, Billerica, MA), rabbit anti-mouse Olig2 monoclonal (1∶250, Millipore). Brightfield immunohistochemistry for Ki67 was performed using a biotinylated anti-mouse secondary antibody (Jackson ImmunoResearch, West Grove, PA) in conjunction an avidin-biotin-peroxidase amplification system (VectaStain ABC Kit, Vector Laboratories, Burlington, ON, Canada) and 3,3-diaminobenzidine (DAB). Brightfield sections were counterstained with CV. Multilabel immunofluorescence was performed by co-incubating cryostat sections with Ki67, Olig2 and Vimentin primary antibodies and detecting them with Alexa 555 goat anti-mouse (1∶1000), Alexa 488 goat anti-rabbit (1∶1000) and Dylight donkey anti-chicken (1∶500) secondary antibodies (Jackson ImmunoResearch). Immunofluorescence sections were counterstained with Hoechst 33342 (0.2 µM, Sigma-Aldrich Canada).

Primary antibodies used were rabbit anti-mDia1 polyclonal (LifeSpan BioSciences, Seattle, WA), rabbit anti-mDia3 polyclonal (SIGMA-Aldrich, St. Louis, MO), chicken anti-vimentin polyclonal (Millipore, Burlington, MA), rabbit anti-GFP polyclonal (MBL, Nagoya, Japan), goat anti-nectin-2 polyclonal (Santa Cruz Biotechnology, Dallas, TX), rabbit anti-espin1 polyclonal (#PB538, a gift from Dr. Bechara Kachar, NIH) [49 (link)], mouse anti-espin1 monoclonal, mouse anti-N-cadherin monoclonal (BD Bioscience, San Jose, CA), and mouse anti-phospho-myosin light chain2 (Ser19) monoclonal (Cell Signaling, Danvers, MA). Inhibitors used were SMIFH2 (Tocris, Bristol, United Kingdom) and CK-666 (Tocris, Bristol, UK).