All solvents used for GC-MS analysis were of analytical grade. Methanol, hexane and ammonium hydroxide were purchased from Tedia (Tedia way, Fairfield, USA), while isopropanol and hydrochloric acid (37%) were purchased from Fisher Scientific (Loughborough, Leicestershire, U.K.), formic acid and myristic-d27 acid were purchased from Sigma-Aldrich (St. Louis, MO, USA, respectively). MSTFA (N-Methyl-N- (trimethylsilyl) trifluoroacetamide) and methoxylamine hydrochloric were purchased from Acros Organic (New Jersey, USA). Deionized water (Milli-Q) was used throughout the study (Millipore, Billerica, MA, USA).
Ammonium hydroxide
Manufactured by Tedia
Sourced in United States, Brazil
Ammonium hydroxide is a colorless, basic aqueous solution of ammonia. It is commonly used as a laboratory reagent and cleaning agent due to its alkaline properties.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Tedia (2 mentions)
Myristic d27 acid, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Thermo Fisher Scientific (1 mentions)
Methoxylamine hydrochloric, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Merck Group (1 mentions)
Hexane, by Tedia (1 mentions)
Milli q device, by Merck Group (1 mentions)
U 13c d glucose, by Cambridge Isotopes (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Ammonium acetate, by Avantor (1 mentions)
Millipore purification system, by Merck Group (1 mentions)
Isotopically labeled internal standards, by Cambridge Isotopes (1 mentions)
Formic acid, by Tedia (1 mentions)
Deuterium oxide, by Cambridge Isotopes (1 mentions)
U 13c l glutamine, by Cambridge Isotopes (1 mentions)
Acetonitrile, by Tedia (1 mentions)
2 protocols using ammonium hydroxide
1
GC-MS Analysis Solvents and Reagents
2
Characterization of Metabolite Standards
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All solvents used were of HPLC analytical grade. Acetonitrile and methanol were obtained from TEDIA® (Fairfield, OH, USA), and isopropanol from Sigma Aldrich (São Paulo, Brazil). Water was purified in the Milli-Q device, the Millipore Purification System (Billerica, MA, USA). Mobile phase additives formic acid and ammonium hydroxide were purchased from TEDIA®, and ammonium acetate was obtained from J. T. Baker® (Aparecida de Goiânia, Brazil). Isotopically labeled internal standards, U 13C D-glucose and U 13C L-glutamine, and deuterium oxide were purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). All other standards were obtained from Sigma Aldrich.
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