In the laboratory, ATE was produced by adding Ringer lactate (Baxter Healthcare Corporation, Helsinki, Finland) to the adipose tissue sample at an approximate ratio of 1:1 and then processed according to the different study variables. Incubation was performed at 37°C water bath or at room temperature (RT). The incubation times studied were 15, 30, or 45 min. After incubation, the samples were sterile filtered with different 0.2 μm pore size syringe filters (Acrodisc® filter, polyethersulfone PES membrane [PALL Life Sciences, New York]; Minisart NML filter, cellulose acetate membrane [Sartorius AG, Germany]; Filtropur S Plus filter, cellulose acetate membrane [Sarstedt & Co, Germany]; and Millex GP filter, PES membrane [Merck, Millipore, Germany]). Once filtered, the ATE was stored at −20°C until sample analysis.
Ringer lactate
Manufactured by Baxter
Ringer lactate is a sterile isotonic solution primarily composed of sodium chloride, potassium chloride, calcium chloride, and sodium lactate. It is commonly used as a replacement fluid for the maintenance of fluid and electrolyte balance in the body.
Automatically generated - may contain errors
3 protocols using ringer lactate
1
Adipose Tissue Extract Preparation
2
Sheep Surgical Monitoring Protocol
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The sheep was placed on its back. During surgery, a rectal temperature probe and a rumen tube were placed. ECG and oxygen saturation were monitored. The sheep was infused with electrolyte solution (Ringer Lactate, Baxter) to maintain isovolumetric conditions.
3
Sheep Surgical Anesthesia and Monitoring
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On the day of surgery, pre-medication was performed by intravenous injection of a mixture of diazepam (Diazepam ® , TVM) and butorphanol (Torphasol ® , Axience). Anesthesia was induced by intravenous injection of propofol (Propovet ® , Zoetis). Each sheep was intubated, mechanically ventilated, and placed on isoflurane inhalant anesthetic (IsoFlo ® , Zoetis) for continued general anesthesia. A suitable electrolyte solution (Ringer lactate, Baxter) was administered via intravenous infusion during surgery. An anti-inflammatory drug (carprofen, Rimadyl ® , Zoetis, subcutaneous) and a prophylactic antibiotic treatment (amoxicillin, Duphamox LA ® , Zoetis, intramuscular) were administered via pre-operative injection. The surgical areas were clipped free of wool, scrubbed with povidone iodine (Vetedine savon ® , Vetoquinol), wiped with 70% isopropyl alcohol (Savetis), painted with povidone iodine solution (Vetedine solution ® , Vetoquinol), and draped. The sheep were placed in the supine position on a warmed pad. A rectal temperature probe and a rumen tube were inserted during surgery. Electrocardiogram (ECG), peripheral non-invasive arterial blood pressure, and oxygen saturation were monitored.
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