The Cyan ADP flow cytometer (Beckman Coulter, Indianapolis IN) was used for all flow samples. Antibodies for these surface markers were used for flow cytometry: CD3 (145-2C11, BD Bioscience Franklin Lakes, NJ, USA), CD4 (RM4-5, BD Bioscience), anti-CEA CAR (Wi2, Immunomedics Morris Plains, NJ, USA), CD11b (M1/17, BD Bioscience), Ly6C (AL-21, BD Bioscience), Ly6G (1AB, BD Bioscience), PD-L1 (MIH5, BD Bioscience), CD62L (MEL-14, BD Bioscience), CCR7 (4B12, BD Bioscience), CD44 (IM7, BD Bioscience). Intracellular FoxP3 staining was performed with Mouse FoxP3 Permeabilization Kit (BD Bioscience). Single stain and isotype controls were used for each experiment. Analysis of acquired flow samples was performed with FlowJo software (Tree Star Inc., Ashland OR).
Cd11b m1 17
Manufactured by BD
Sourced in United States
CD11b (M1/70) is a cell surface integrin receptor that is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. This protein plays a crucial role in cell adhesion, migration, and phagocytosis. It serves as a marker for the identification and investigation of these immune cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 145 2c11, by BD (2 mentions)
Cd4 rm4 5, by BD (2 mentions)
Cd62l mel 14, by BD (2 mentions)
Ly6g 1ab, by BD (2 mentions)
Pd l1 mih5, by BD (2 mentions)
Ccr7 4b12, by BD (2 mentions)
Cd44 im7, by BD (2 mentions)
Mouse foxp3 permeabilization kit, by BD (2 mentions)
Ly6c al 21, by BD (2 mentions)
Cyan adp flow cytometer, by Beckman Coulter (2 mentions)
2 protocols using cd11b m1 17
1
Comprehensive Multiparameter Flow Cytometry
2
Comprehensive Multiparameter Flow Cytometry
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The Cyan ADP flow cytometer (Beckman Coulter, Indianapolis IN) was used for all flow samples. Antibodies for these surface markers were used for flow cytometry: CD3 (145-2C11, BD Bioscience Franklin Lakes, NJ, USA), CD4 (RM4-5, BD Bioscience), anti-CEA CAR (Wi2, Immunomedics Morris Plains, NJ, USA), CD11b (M1/17, BD Bioscience), Ly6C (AL-21, BD Bioscience), Ly6G (1AB, BD Bioscience), PD-L1 (MIH5, BD Bioscience), CD62L (MEL-14, BD Bioscience), CCR7 (4B12, BD Bioscience), CD44 (IM7, BD Bioscience). Intracellular FoxP3 staining was performed with Mouse FoxP3 Permeabilization Kit (BD Bioscience). Single stain and isotype controls were used for each experiment. Analysis of acquired flow samples was performed with FlowJo software (Tree Star Inc., Ashland OR).
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