HEK-293 cells were divided into the miR-335-5p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO- MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5ʹ-cATTTAACTTCTAGTTGCTCTTGCc-3ʹ and down 5ʹ-tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3ʹ; and mutant MAPK10, up 5ʹ-cATTTAACTTCTAGTTGATATCGCc-3ʹ and down 5ʹ-tcgagGCGATATCAACTAGAAGTTAAATgagct-3ʹ. Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
Mutant mapk10
Manufactured by Genechem
Sourced in China
Mutant MAPK10 is a laboratory product that serves as a key component in cellular signaling research. It is a variant of the mitogen-activated protein kinase 10 (MAPK10) enzyme, which plays a crucial role in various cellular processes. This mutant form of MAPK10 can be utilized by researchers to study the impact of specific genetic modifications on cellular function and signaling pathways.
Automatically generated - may contain errors
3 protocols using mutant mapk10
1
Investigating miR-335-5p and MAPK10 Interaction
2
Cell Cycle Analysis and Luciferase Assay
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Transfected cells in the logarithmic growth phase were inoculated onto a 6-well plate and cultured for 1 day. Cells were xed in 70% ethanol for 24 h and treated with propidium iodide and RNase provided with the kit. The cell cycle distribution was detected by ow cytometry.
Dual Luciferase Reporter Assay HEK-293 cells were divided into the miR-30a-3p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO-MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5 -cATTTAACTTCTAGTTGCTCTTGCc-3 and down 5 -tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3 ; and mutant MAPK10, up 5 -cATTTAACTTCTAGTTGATATCGCc-3 and down 5 -tcgagGCGATATCAACTAGAAGTTAAATgagct-3 . Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
Dual Luciferase Reporter Assay HEK-293 cells were divided into the miR-30a-3p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO-MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5 -cATTTAACTTCTAGTTGCTCTTGCc-3 and down 5 -tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3 ; and mutant MAPK10, up 5 -cATTTAACTTCTAGTTGATATCGCc-3 and down 5 -tcgagGCGATATCAACTAGAAGTTAAATgagct-3 . Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
3
Dual Luciferase Assay for miRNA-MAPK10 Interaction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transfected cells in the logarithmic growth phase were inoculated onto a 6-well plate and cultured for 1 day. Cells were xed in 70% ethanol for 24 h and treated with propidium iodide and RNase provided with the kit. The cell cycle distribution was detected by ow cytometry.
Dual Luciferase Reporter Assay HEK-293 cells were divided into the miR-335-5p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO-MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5 -cATTTAACTTCTAGTTGCTCTTGCc-3 and down 5 -tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3 ; and mutant MAPK10, up 5 -cATTTAACTTCTAGTTGATATCGCc-3 and down 5 -tcgagGCGATATCAACTAGAAGTTAAATgagct-3 . Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
Dual Luciferase Reporter Assay HEK-293 cells were divided into the miR-335-5p and pmirGLO empty vectors, miR-335-5p and pmirGLO-MAPK10-WT (GenePharma), miR-335-5p, and pmirGLO-MAPK10-MuT (GenePharma) co-transfection groups, respectively. Untreated cells were used as controls. Wild-type and mutant MAPK10 were synthesized by GeneChem (Shanghai, China) as follows: wild-type MAPK10, up 5 -cATTTAACTTCTAGTTGCTCTTGCc-3 and down 5 -tcgagGCAAGAGCAACTAGAAGTTAAATgagct-3 ; and mutant MAPK10, up 5 -cATTTAACTTCTAGTTGATATCGCc-3 and down 5 -tcgagGCGATATCAACTAGAAGTTAAATgagct-3 . Cells were inoculated onto 96-well plates and cultured for 24 h. Luciferase activity was detected using a microplate reader. Renilla luciferase was used as an internal reference.
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