Stained transverse sections of tibiae were viewed by an eclipse E400 Nikon light microscopy with ×4, ×10, ×20, or ×40 objectives, using light filters. Images were taken by a high-resolution camera (Olympus DP 71) controlled by Cell A software (Olympus, Japan). The thickness of the entire GPs, the proliferative zone (PZ), and the hypertrophic zone (HZ) as well as the number of cells were measured using the Cell A software with a measuring tool feature. Measurements were performed on these sections from 4 different animals from each group. In each slide, 10 random locations throughout the GPs were selected and measured [32 (link),33 (link)].
Eclipse e400 light microscopy
Manufactured by Nikon
The Eclipse E400 is a Nikon light microscopy system designed for laboratory use. It provides optical imaging capabilities for various scientific and research applications. The core function of the Eclipse E400 is to enable high-quality, clear observation and analysis of specimens under magnification.
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Lab products found in correlation
2 protocols using eclipse e400 light microscopy
1
Histological Analysis of Tibial Growth Plates
2
Histomorphometric Analysis of Tibial Growth Plates
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Stained transverse sections of tibiae were viewed by an eclipse E400 Nikon light microscopy with ×4, ×10, ×20 or ×40 objectives, using light filters. Images were taken by a high -resolution camera (Olympus DP 71) controlled by Cell A software (Olympus, Japan). The thickness of the entire GPs, the proliferative zone (PZ) and the hypertrophic zone (HZ) as well as the number of cells were measured using the Cell A software with a measuring tool feature. Measurements were performed on these sections from 5 different animals from each group. In each slide, 10 random locations throughout the GPs were selected and measured [28 (link),29 (link)].
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