We measured urinary protein concentrations for each of the proteins of interest using monospecific ELISAs, in accordance with the instructions from the manufacturers. To measure each urinary protein concentration, we used the respective ELISA kits for trefoil factor 1 (TFF1) (#EK1232; Boster Biological Technology, Pleasanton, CA), a disintegrin and metalloproteinase domain-containing protein 12 (ADAM12) (#.DAD120; R&D Systems, Minneapolis, MN), pepsinogen 3 (PGA3) (#OKEH03169; AVIVA SYSTEMS BIOLOGY, San Diego, CA), BRCA1-associated RING domain 1 (BARD1) (#.MBS7236583; MyBioSource, San Diego, CA), coiled coil domain-containing protein 38 (CCDC38) (#.MBS7209768; MyBioSource), tubulointerstitial nephritis antigen-like 1 (TINAGL1) (#MBS9340283; MyBioSource), DEAD-box helicase 55 (DDX55) (#MBS7244207; MyBioSource) and a Parameter Creatinine Assay (R&D Systems) for creatinine. Each ELISA analysis required 1–100 μl of urine or serum sample.
Parameter creatinine assay
Manufactured by R&D Systems
Sourced in Canada, United States
The Parameter Creatinine Assay is a quantitative colorimetric assay designed to measure the concentration of creatinine in biological samples. The assay utilizes a specific enzymatic reaction to produce a colored product, the intensity of which is proportional to the creatinine concentration in the sample. This assay provides a reliable and accurate method for the determination of creatinine levels.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using parameter creatinine assay
1
Quantifying Urinary Protein Biomarkers
2
Urinary Protein Quantification via ELISAs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We measured the urinary protein concentration of each of the proteins of interest using mono-specific ELISAs, in accordance with the instructions from the manufacturers. To measure each protein concentration, we used a Quantikine ELISA kit (R&D Systems, Minneapolis, MN) for cysteine-rich protein 61 (Cyr61) and trefoil factor 3 (TFF3), a SimpleStep ELISA Kit (Abcam, Cambridge, UK) for insulin-like growth factor-binding protein 3 (IGFBP3) and alpha 1 antitrypsin (SERPINA1), a human EGF-containing fibulin-like extracellular matrix protein 2 (EFEMP2) ELISA kit (LSBio, Seattle, WA) for EFEMP2, a human angiopoietin-like protein 2 (ANGPTL2) ELISA kit (Aviva Systems Biology, San Diego, CA) for ANGPLT2 and a Parameter Creatinine Assay (R&D Systems) for creatinine. All urinary protein levels were normalized to urinary creatinine.
3
Urinary Protein Quantification Using ELISAs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We measured the urinary protein concentration of each of the proteins of interest using mono-specific enzyme-linked immunosorbent assays (ELISAs), according to the manufacturer's instructions. To measure each protein concentration, we used a Quantikine ELISA kit (R&D Systems, Inc.) for MMP-9, a DuoSet ELISA with the DuoSet Ancillary Reagent Kit 2 (R&D Systems, Inc.) for PRTN3, a human kallikrein 10 ELISA kit (RayBiotech, Inc., Norcross, GA, USA) for KLK10, and a Parameter Creatinine Assay (R&D Systems, Inc.) for creatinine. All urine samples were measured in duplicate, and the mean was obtained.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!