Epstein−Barr virus (EBV)-negative NPC cell lines (CNE2, HONE1, and 5–8F) were generously provided by Professor Musheng Zeng (Sun Yat-sen University Cancer Center, Guangzhou, China). The EBV-positive NPC cell line (HK1-EBV) and two immortalized normal human nasopharyngeal epithelial cell lines (NP460hTert-EBV and NP460hTert) were kindly provided by Professor George S. W. Tsao (University of Hong Kong). The cell lines were confirmed as negative for mycoplasma contamination (Qiagen, Hilden, Germany). All NPC cell lines were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% newborn cow serum (Gibco, Gaithersburg, MD, USA) at 37°C and 5% CO2. NP460hTert-EBV and NP460hTert cells were cultured in defined keratinocyte serum‐free medium (Invitrogen) at 37°C and 5% CO2. All gifted cell lines were authenticated by STR profile.
Mycoplasma contamination
Manufactured by Qiagen
Sourced in Germany
Mycoplasma contamination is a lab equipment product designed to detect and monitor mycoplasma contamination in cell cultures. It provides a reliable and efficient method to ensure the integrity of cell-based research and production processes.
Automatically generated - may contain errors
Lab products found in correlation
Defined keratinocyte serum free medium, by Thermo Fisher Scientific (2 mentions)
Newborn cow serum, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Interleukin 6 (il 6), by Novoprotein (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Stattic, by Selleck Chemicals (1 mentions)
2 protocols using mycoplasma contamination
1
Characterization of Nasopharyngeal Carcinoma Cell Lines
2
Characterization of EBV-negative NPC Cell Lines
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Five EBV-negative NPC cell lines, namely CNE1, CNE2, HONE1, 5–8F, 6–10B, were generously provided by Prof. Musheng Zeng (Sun Yat-sen University Cancer Center, China). The HK1-EBV and two immortalized normal human nasopharyngeal epithelial cell lines NP460hTert-EBV and NP460hTert were provided by Prof. George S.W. Tsao, University of Hong Kong, China. The cell lines tested negative for mycoplasma contamination (Qiagen, Germany). The NPC cell lines were cultured in RPMI-1640 (Invitrogen, USA) supplemented with 10% newborn cow serum (Gibco, USA); NP460hTert-EBV and NP460hTert, defined keratinocyte serum‐free medium (Invitrogen); and 293T cells, DMEM (Invitrogen) supplemented with 10% newborn cow serum (Gibco). The cell lines were incubated in a humidified chamber with 5% CO2 at 37 °C. All gifted cell lines were authenticated by STR profile. Barflomycin A1 (Baf-A1; Selleckchem, USA), Stattic (Selleckchem), and IL-6 (Novoprotein, USA) were dissolved according to the manufacturers’ instructions. The antibodies used for subsequent experiments are listed in Table S4 .
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